Page 97 - 《精细化工》2020年第9期

第 9 期 刘庆宇，等: 基于 1,8-萘酰亚胺检测细胞内肼的荧光探针 ·1811·

a、b、c 为未添加肼的探针与细胞孵育 30 min 后的细胞图；d、e、f 为探针与细胞共同孵育 30 min 后再添加肼（100 μmol/L）
处理 30 min 的细胞图；1、2、3、4 分别为 Hela 细胞、A549 细胞、MCF-7 细胞、MDA-MB 231 细胞；a 和 d 为白光图；

b 和 e 为荧光图；c 和 f 为合并图像

图 11 探针 NH1(10 μmol/L)在不同细胞中的共聚焦成像图
Fig. 11 Confocal fluorescent images of probe NH1(10 μmol/L) in different cells

如图 11 所示，观察两组实验的共聚焦成像发 aqueous solution[J]. Dyes and Pigments, 2013, 99(3): 966-971.
[8] LIU L, LE Y, TENG M G, et al. A novel fluorescence sensor for
现，第一组未加肼的细胞显示出微弱的绿色荧光； hydrazine based on pyrazole formation reaction[J]. Dyes and
而在第二组加入肼后的细胞中观察到了强烈的绿色 Pigments, 2018, 151: 1-6.
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荧光。这说明探针 NH1 能够实现细胞中的染色，并 colorimetric fluorescent probe for hydrazine based on ring-opening
且在细胞样品中进行肼的检测是可行的。 reaction and its applications[J]. Sensors and Actuators B: Chemical,
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开发了一种以萘酰亚胺为荧光母体检测肼的荧 [11] SHI X L, YIN C X, WEN Y, et al. A probe with double acetoxyl
moieties for hydrazine and its application in living cells[J].
光探针 NH1。该探针具有选择性好、灵敏度高、抗 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,
干扰能力强等优点。并且能够在较大的 pH 范围 2018, 203: 106-111.
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（4.5~10.5）内稳定检测肼。探针荧光强度与肼在 fluorescent probe for quantitive detection and imaging of alkaline
2
0~40 μmol/L 的范围内有着良好的线性关系（R = phosphatase in living cells[J]. Analytica Chimica Acta, 2019, 1066:
131-135.
0.99），检测限为 0.23 μmol/L（<0.31 μmol/L）。探针 [13] GUO S H, GUO Z Q, WANG C Y, et al. An ultrasensitive fluorescent
对肼响应迅速，响应时间为 80 s。通过实验验证了 probe for hydrazine detection and its application in water samples
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探针 NH1 响应肼的机理。将探针应用于细胞内肼的 [14] BAI H Y, QIAN J H, TIAN H Y, et al. Fluorescent polarity probes for
检测表明，探针 NH1 有着较好的生物应用前景。 identifying bovine serum albumin: Amplification effect of
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colorimetric probe for trace hydrazine with a wide detection range in （下转第 1832 页）