Page 104 - 《精细化工》2021年第10期
P. 104
第 38 卷第 10 期 精 细 化 工 Vol.38, No.10
2021 年 10 月 FINE CHEMICALS Oct. 2021
功能材料
2+
基于 BODIPY 荧光探针对 Cu 和
焦磷酸根的连续识别
孟宪娇
(山西农业大学 基础部,山西 晋中 030801)
摘要:以 8-羟基喹啉-5-甲醛和吡啶-2, 6-二甲酸二甲酯为原料,合成了一种氟硼二吡咯(BODIPY)型荧光化学
1
13
传感器Ⅰ,通过 FTIR、 HNMR、 CNMR 和 ESI-MS 对其结构进行了表征,利用紫外/荧光分光光度法研究了传
2+
感器Ⅰ的传感性能。结果表明,在 V(DMF)∶V(水)=1∶4 介质中,传感器Ⅰ对 Cu 表现出明显的荧光猝灭响应,
–8
2+
猝灭率高达 97.7%,检测限为 5.7×10 mol/L。此外,配合物Ⅰ-Cu 对焦磷酸根(PPi)表现出明显的荧光增强响应,
2+
–8
检测限为 1.9×10 mol/L,表明传感器Ⅰ对 Cu 和 PPi 的识别检测具有较高的灵敏度和抗干扰能力。通过络合曲
2+
2+
线和核磁滴定数据分析,得出传感器Ⅰ同 Cu 以及配合物Ⅰ-Cu 对 PPi 的络合物质的量比均为 1∶1。传感器Ⅰ
2+
对 Cu 和 PPi 测试的有效 pH 范围为 5~9,且循环响应测试可稳定进行 4 次。
2+
关键词:氟硼二吡咯;荧光探针;Cu ;焦磷酸根;连续识别;功能材料
中图分类号:TQ422+.2;TP212.3;S951.4+2 文献标识码:A 文章编号:1003-5214 (2021) 10-2034-09
Fluorescent probe based on BODIPY for sequential recognition
2+
of Cu and pyrophosphate
MENG Xianjiao
(Basic Sciences Depart, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China)
Abstract: A fluorescent chemical sensor based on fluoroboron dipyrrole (BODIPY) (Ⅰ) was synthesized
using 8-hydroxyquinoline-5-carbaldehyde and dimethyl pyridine-2, 6-dicarboxylate as raw materials. The
13
1
structure was characterized by FTIR, HNMR, CNMR and ESI-MS. The sensing performance of sensor
Ⅰwas studied by UV-Vis/fluorescence spectrophotometry. The results showed that sensorⅠhad obviously
2+
“on-off” fluorescence response to Cu in V(DMF)∶V(water)=1∶4 solution. The quenching efficiency
–8
2+
reached a maximum of 97.7%, and the detection limit of sensorⅠto Cu was 5.7×10 mol/L. Moreover,
2+
complexⅠ-Cu exhibited obviously “off-on” fluorescence response to pyrophosphate (PPi) with a
–8
detection limit of 1.9×10 mol/L, indicating that sensorⅠpossessed high sensitivity and anti-interference
2+
1
ability in recognition and detection of Cu and PPi. Job′s plots and HNMR titration data revealed that the
2+
2+
complexing mole ratios of sensorⅠto Cu and complexⅠ-Cu to PPi were 1∶1. The effective pH range
2+
of sensorⅠto Cu and PPi was in the range from 5 to 9, and the cycle response test could reach 4 times.
2+
Key words: fluoroboron dipyrrole; fluorescent probe; Cu ; PPi; sequential recognition; functional materials
2+
Cu 作为人体中含量较多的过渡金属,其含量 症和威尔逊氏病等疾病的发生 [2-3] 。因此,对于 Cu 2+
[1]
的多少对生命体的健康起着至关重要的作用 。例 的识别检测也引起了广泛关注 [4-6] 。PPi 作为三磷酸
2+
如,Cu 含量过低容易引起贫血和骨质疏松症等疾 腺苷(ATP)主要的水解产物之一,参与着人体内多种
2+
病,而 Cu 含量过高又会导致与神经相关的帕金森 至关重要的反应过程,异常的含量往往会引发多种
收稿日期:2021-05-14; 定用日期:2021-09-23; DOI: 10.13550/j.jxhg.20210493
基金项目:山西农业大学科技创新项目(2020BQ18);山西省优秀博士来晋工作奖励(SXYBKY2019046);山西省高等学校创新项目
(2020L0140)
作者简介:孟宪娇(1987—),女,讲师,E-mail:513091702@qq.com。