Page 103 - 《精细化工》2021年第12期

第 38 卷第 12 期 精 细 化 工 Vol.38, No.12
2021 年 12 月 FINE CHEMICALS Dec. 2021

功能材料
负载活性组分的核壳结构纤维膜的制备及性能

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郝中乾 ，王曼婷 ，张成桂 ，乐 园 1,2*
（1. 北京化工大学 有机-无机复合材料国家重点实验室，北京 100029；2. 北京化工大学 教育部超重力
工程研究中心，北京 100029；3. 大理大学 云南省昆虫生物医药研发重点实验室，云南 大理 671000）

摘要：采用同轴静电纺丝技术制备了用于伤口修复的核壳结构纳米纤维膜，将蛛丝蛋白（Ss）和美洲大蠊提取
物（PAE）分别负载于纳米纤维的壳层与核层。采用 SEM 和 TEM 对纳米纤维膜的形貌进行了表征，结果显示，
纤维具有明显的核壳结构，且随着 Ss 含量的增加，纤维直径从 350 nm 降至 280 nm，核层直径由 120 nm 升至
140 nm，壳层厚度由 115 nm 降至 70 nm；FTIR 结果证明 Ss 已成功负载到纤维膜中。纤维膜的物理性能测定实
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验表明，制备的纳米纤维膜拉伸强度可达 4.3 MPa，溶胀率可达 150%，水蒸气透过率可达 1834 g/(m ·24 h)，水
接触角减小到 32.7°。药物释放实验结果显示，7 d 内药物释放可达 77%；考察了纳米纤维膜的生物相容性，相
较于未负载 Ss 的纳米纤维膜，Ss 含量为 20%的纤维膜的细胞增殖率提高了 25%。
关键词：同轴静电纺丝；蛛丝蛋白；美洲大蠊提取物；核壳结构；药物缓释；功能材料
中图分类号：TQ340.64；R318.08 文献标识码：A 文章编号：1003-5214 (2021) 12-2465-07

Preparation and performance of core-shell structure fiber

membranes loaded with active components

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HAO Zhongqian , WANG Manting , ZHANG Chenggui , LE Yuan 1,2
（1. State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing
100029, China; 2. Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing
University of Chemical Technology, Beijing 100029, China; 3. Yunnan Provincial Key Laboratory of Entomological
Biopharmaceutical R&D, Dali University, Dali 671000, Yunnan, China）

Abstract: Nanofiber membranes with core-shell structure for wound repair were prepared by coaxial
electrospinning technique. Spider silk protein (Ss) and Periplaneta americana Linnaeus extract (PAE) were
respectively loaded in the shell and core layer of nanofiber. The morphology of nanofiber membrane was
characterized by SEM and TEM. SEM and TEM results revealed that the fiber had an obvious core-shell
structure. With the increase of Ss content, the diameter of nanofiber decreased from 350 nm to 280 nm, the
diameter of core increased from 120 nm to 140 nm, and the thickness of shell decreased from 115 nm to 70
nm. FTIR results verified the successful loading of Ss into the nanofiber membrane. The physical properties
of nanofiber membrane were determined and the results indicated that the tensile strength could reach 4.31
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MPa, the swelling rate could reach 150%, the water vapor permeability rate could reach 1834 g/(m ·24 h)
and the water contact angle could be reduced to 32.7°. The results of drug release experiment showed that
drug release could reach 77% within 7 d. The biocompatibility of nanofiber membrane was also
investigated. Compared with that of unloaded with Ss, cell proliferation of nanofiber membrane with 20%
content Ss was increased by 25%.
Key words: coaxial electrospinning; spider silk protein; Periplaneta americana Linnaeus extract; core-shell
structure; slow drug release; functional materials

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皮肤是保卫人体的第一道防线 。伤口愈合主要 分为止血、炎症、细胞增殖和组织重塑 4 个阶段 。

收稿日期：2021-06-17; 定用日期：2021-07-05; DOI: 10.13550/j.jxhg.20210621
作者简介：郝中乾（1994—），男，硕士生，E-mail：haozhongqian@163.com。联系人：乐 园（1967—），女，教授，E-mail：
leyuan@mail.buct.edu.cn。