Page 154 - 《精细化工》2022年第2期

第 39 卷第 2 期 精 细 化 工 Vol.39, No.2
2022 年 2 月 FINE CHEMICALS Feb. 2022

医药与日化原料
7,8-二羟基黄酮纳米粒子通过保护

卵巢储备缓解代谢综合征

1
2
2*
1
赵镇雷 1,2 ，薛 凡 ，徐小刚 ，毛根祥 ，张 英
（1. 浙江医院 浙江省老年医学研究所&浙江省老年医学重点实验室，浙江 杭州 310013；2. 浙江大学 生
物系统工程与食品科学学院，浙江 杭州 310058）
摘要：以芜菁多糖构建的 7,8-二羟基黄酮（7,8-DHF）纳米粒子对两种不同膳食模式（高脂和低脂）饲养的
C57BL/6J 雌性小鼠进行 24 周的干预，采用分子生物学方法对代谢综合征（MetS）各项指标、炎症因子及卵巢
储备功能进行了测定，探究了其对小鼠 MetS 的改善作用和机制。结果表明，7,8-DHF 及其纳米粒子均可有效缓
解小鼠 MetS 的症状，可显著降低高脂膳食（HFD）诱导的小鼠体重过度增长，与 HFD 对照组相比，7,8-DHF
及 7,8-DHF 纳米粒子组体重分别下降了 8.5%和 16.4%；可显著改善血脂、血糖及血胰岛素水平、小鼠的葡萄糖
耐受性和胰岛素敏感性；能够显著降低血清中循环脂多糖、肿瘤坏死因子（TNF-α）和白细胞介素-6（IL-6）等
炎症相关指标；可以有效保护小鼠卵巢储备功能，维持其正常的动情周期和血清雌二醇、卵泡刺激素的水平稳
态。7,8-DHF 纳米粒子对 MetS 各项指标的干预效果显著优于单独的 7,8-DHF。7,8-DHF 及其纳米粒子对雌性小
鼠 MetS 的干预作用可能是通过缓解肠道菌群介导的全身炎症并作用于对卵巢储备功能的保护而实现的。
关键词：7,8-二羟基黄酮；芜菁多糖；纳米粒子；代谢综合征；卵巢储备；中药活性成分；医药原料
中图分类号：R285 文献标识码：A 文章编号：1003-5214 (2022) 02-0358-09

7,8-Dihydroxyflavone nanoparticles alleviating metabolic syndrome
by protecting ovarian reserve

1
2*
1
1,2
2
ZHAO Zhenlei , XUE Fan , XU Xiaogang , MAO Genxiang , ZHANG Ying
（1. Zhejiang Provincial Key Lab of Geriatrics and Geriatrics Institute of Zhejiang Province, Department of Geriatrics,
Zhejiang Hospital, Hangzhou 310013, Zhejiang, China; 2. College of Biosystems Engineering and Food Science,
Zhejiang University, Hangzhou 310058, Zhejiang, China）
Abstract: 7, 8-Dihydroxyflavone (7,8-DHF) nanoparticles constructed from turnip polysaccharides were
used to intervene C57BL/6J female mice fed two different dietary patterns (high fat and low fat) for 24
weeks. Metabolic syndrome (MetS) indicators, inflammatory factors and ovarian reserve function were
measured using molecular biological methods after intervention. The improvement effect and mechanism of
MetS in mice were explored. It was found that both 7,8-DHF and its nanoparticles could alleviate
symptoms of MetS. Compared with that of high fat diet (HFD) control group, the body mass of 7,8-DHF
and 7,8-DHF nanoparticles treated groups decreased by 8.5% and 16.4%, respectively. In addition, they
could obviously improve blood lipid, fasting blood glucose and insulin levels, glucose tolerance and insulin
sensitivity of mice, obviously decrease the serum levels of circulating lipopolysaccharide, tumor necrosis
factor alpha and interleukin-6. Moreover, they could effectively protect the ovarian reserve function of mice,
maintain the normal estrous cycle and the homeostasis of serum estradiol and follicle-stimulating hormone.
7,8-DHF nanoparticles exhibited better anti-MetS effect than 7,8-DHF alone. The intervention of 7,8-DHF

收稿日期：2021-07-13; 定用日期：2021-10-08; DOI: 10.13550/j.jxhg.20210714
基金项目：国家自然科学基金（31702144）
作者简介：赵镇雷（1990—），男，博士，E-mail：zhenlei@zju.edu.cn。联系人：张英（1961—），女，教授，博士生导师，E-mail：
yzhang@zju.edu.cn。