Page 88 - 《精细化工》2022年第4期

·724· 精细化工 FINE CHEMICALS 第 39 卷

柠檬酸作为碳源使用。
芦丁类似结构的猝灭实验有待探索，自然界中
四季常青的绿色碳源有待发掘，测试方法的适用范
围有待拓展，CDs 表面结构与猝灭剂间的相互作用
机制也有待进一步探索。

参考文献：
[1] SHARMA S, ALI A, ALI J, et al. Rutin: Therapeutic potential and
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[2] GULLON B, LUCHAU T A, MOREIRA M T, et al. Rutin: A review

on extraction, identification and purification methods, biological
图 10 芦丁对柠檬酸 CDs 的猝灭光谱曲线（a）及柠檬酸 activities and approaches to enhance its bioavailability[J]. Trends in
Food Science & Technology, 2017, 67: 220-235.
CDs 对芦丁的响应标准曲线（b）
[3] MAO Y J (毛雅君), FENG Y L (冯亚莉), WANG M J (王梦娇).
Fig. 10 Quenching spectra of citric acid CDs by rutin (a) Research progress on rutin derivatives[J]. China Journal of Chinese
and response standard curve of CDs to rutin (b) Materia Medica (中国中药杂志), 2021, 46(18): 4654-4665.
[4] LIU D G, MEI Q, WAN X L, et al. Determination of rutin and
2.6 CDs 荧光探针检测槐米中的芦丁含量 isoquercetin contents in Hibisci mutabilis folium in different
取 1 g 槐米粉末，加入 30 mL 甲醇，50 ℃水浴 collection periods by HPLC[J]. Journal of Chromatographic Science,
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反应 1 h，过滤除去残渣，用甲醇定容至 50 mL，浓 [5] SUN Y, GUO T, SUI Y, et al. Quantitative determination of rutin,
度记为 c H0 。测定时，用超纯水稀释，测定浓度为 quercetin, and adenosine in flos carthami by capillary
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c H0 /100 中的芦丁含量，CDs 荧光探针检测槐米提取 1203-1206.
液中的芦丁含量结果如表 1 所示。 [6] WANG W, LIN P, MA L H, et al. Separation and determination of
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electrophoresis with amperometric detection[J]. Journal of Separation
表 1 槐米提取液测定结果 Science, 2016, 39(7): 1357-1362.
Table 1 Analysis results of Sophora japonica buds extracts [7] LI S M, YANG B B, WANG C Q, et al. A facile and green fabrication
of Cu 2O-Au/NG nanocomposites for sensitive electrochemical
芦丁含量 加标量 RSD(n=3)
样品 回收率/% determination of rutin[J]. Journal of Electroanalytical Chemistry,
/(μmol/L) /(μmol/L) /%
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银杏叶 CDs 15.50 5.0 95.32 3.4 [8] GAO Y D, WANG L, ZHANG Y L, et al．Greenly synthesized
10.0 98.98 3.2 graphene with L-glutathione-modified electrode and its application
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15.0 93.33 2.6
94032.
30.0 103.77 1.9 [9] SONG Z H, HOU S. Sensitive determination of sub-nanogram
柠檬酸 CDs 15.96 5.0 97.87 3.2 amounts of rutin by its inhibition on chemiluminescence with
immoblized reagents[J]. Talanta, 2002, 57(1): 59-67.
10.0 101.59 2.1
[10] LI S F, ZHANG L, CHENG L, et al. Determination of rutin by
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30.0 104.35 1.2 system[J]. Analytical Methods, 2016, 8(20): 4056-4063.
[11] LIU S, TIAN J Q, WANG L, et al. Hydrothermal treatment of grass:
注：RSD 为相对标准偏差。 A low-cost, green route to nitrogen-doped, carbon-rich,
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由表 1 可知，两种 CDs 荧光探针测定浓度为 sensing platform for label-free detection of Cu(Ⅱ) ions[J]. Advanced
c H0 /100 的提取液中芦丁含量，测定结果基本一致。 Materials, 2012, 24(15): 2037-2041.
[12] LIU Y H (刘艳红), ZHANG D X (张东旭), MAO B D (毛宝东),
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3 结论 dots[J]. Acta Chimica Sinica (化学学报), 2020, 78(12): 1349-1365.
[13] GAO X (高雪), SUN J (孙靖), LIU X (刘晓). Carbon quantum dots:
采用一步水热法制备了荧光强度高、发光性能 Synthesis, properties and applications[J]. Chemical Industry and
Engineering Progress (化工进展), 2017, 36(5): 1734-1742.
稳定的银杏叶 CDs 和柠檬酸 CDs，用以检测芦丁含 [14] LI S H, SU W, WU H, et al. Targeted tumour theranostics in mice via
量，具有灵敏度高、选择性强的优点。 carbon quantum dots structurally mimicking large amino acids[J].
Nature Biomedical Engineering, 2020, 4(7): 704-716.
基于芦丁浓度与银杏叶 CDs、柠檬酸 CDs 荧光
[15] PANWAR N, SOEHARTONO A M, CHAN K K, et al. Nanocarbons
猝灭程度之间的线性关系，构建了 CDs 荧光探针， for biology and medicine: Sensing, imaging, and drug delivery[J].
Chemical Reviews, 2019, 119(16): 9559-9656.
用于快速定量分析芦丁含量，方法重现性好。
[16] LIU J H, LI J Z, XU L Q, et al. Facile synthesis of N, B-doped
银杏叶和柠檬酸 CDs 荧光探针检测槐米中的芦 carbon dots and their application for multisensor and cellular
丁含量，结果基本一致，证明此法用于实际样品中 imaging[J]. Industrial & Engineering Chemistry Research, 2017,
56(14): 3905-3912.
芦丁的测定是可靠的，银杏叶可以取代分析纯试剂 （下转第 733 页）