Page 83 - 《精细化工》2022年第4期

第 39 卷第 4 期 精 细 化 工 Vol.39, No.4
2022 年 4 月 FINE CHEMICALS Apr. 2022

功能材料
碳点荧光探针的合成及其对芦丁含量的检测

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丁志杰，程禄军，魏居孟 ，柏 雷，熊江浩，胡祥辉
（安徽科技学院 化学与材料工程学院，安徽 蚌埠 233000）

摘要：以新鲜银杏叶为碳源，采用水热法制备出水溶性好、稳定性高的银杏叶碳点（CDs）。通过 TEM、FTIR、
XRD、UV-Vis 和荧光光谱表征了银杏叶 CDs 的结构及光学特征。基于芦丁对 CDs 荧光的猝灭现象，构建了高
选择性与高灵敏度的荧光探针测试芦丁含量。结果表明，10~80 μmol/L 的芦丁与 CDs 的荧光猝灭程度之间呈现
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良好的线性关系，线性相关系数 R 为 0.9995，检测限为 1.5 μmol/L。另以柠檬酸为碳源，制备了柠檬酸 CDs，
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其猝灭程度与芦丁在同样的浓度范围内呈线性关系，线性相关系数 R 为 0.9996，检测限为 1.7 μmol/L。两种 CDs
荧光探针用于检测槐米中的芦丁含量，检测结果一致。荧光寿命测试证明，银杏叶 CDs、柠檬酸 CDs 与芦丁的
作用机理分别为动态猝灭和静态猝灭。
关键词：银杏叶碳点；柠檬酸碳点；水热法；荧光猝灭；芦丁；功能材料
中图分类号：O657.3；TB34 文献标识码：A 文章编号：1003-5214 (2022) 04-0719-07

Synthesis of carbon dots fluorescent probe and its application
in determination of rutin content

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DING Zhijie, CHENG Lujun, WEI Jumeng , BAI Lei, XIONG Jianghao, HU Xianghui
（College of Chemical and Materials Engineering, Anhui Science and Technology University, Bengbu 233000, Anhui, China）

Abstract: Fresh ginkgo leaves were used as carbon source to synthesize carbon dots (CDs) with good water
solubility and high stability by hydrothermal method. The structure and optical properties of ginkgo leaves
CDs were characterized by TEM, FTIR, XRD, UV-Vis and fluorescence spectra. Based on the quenching of
CDs fluorescence by rutin, a fluorescence probe with high selectivity and sensitivity was constructed to
measure the content of rutin. The results showed that there was a good linear relationship between
fluorescence quenching degree of CDs and rutin from 10 μmol/L to 80 μmol/L, the linear correlation
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coefficient (R ) was 0.9995, and the detection limit was 1.5 μmol/L. Moreover, citric acid CDs was
synthesized using citric acid as carbon source. Likewise, the fluorescence quenching degree of citric acid
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CDs presented a good linear relationship with rutin concentration in the same range. R was 0.9996 and the
detection limit was 1.7 μmol/L. These two fluorescent probes were applied to determine rutin in sophora
flower bud, and a close result was obtained. The fluorescence lifetime test revealed that the interaction
mechanisms of gingko leaf CDs and citric acid CDs with rutin was dynamic quenching and static quenching,
respectively.
Key words: ginkgo leaf carbon dots; citric acid carbon dots; hydrothermal method; fluorescence quenching;
rutin; functional materials

芦丁（Rutin）属多羟基黄酮类化合物，是一种 高”、脑血栓，对视网膜出血、紫癜和急性出血性肾
具有生物活性的天然药物，可用于预防及治疗“三 炎等疾病也有疗效 [1-3] 。除用作药物外，研究者还基

收稿日期：2021-09-29; 定用日期：2021-12-23; DOI: 10.13550/j.jxhg.20210995
基金项目：安徽省自然科学基金青年基金（1908085QE221）；安徽省高校自然科学研究项目（KJ2020A0054）；高校优秀青年人才支
持计划（gxyq2021201）；大学生创新创业训练计划（201910879112）
作者简介：丁志杰（1972—），女，副教授，E-mail：569544200@qq.com。联系人：魏居孟（1985—），男，副教授，E-mail：
weijm@ahstu.edu.cn。