Page 116 - 《精细化工》2022年第7期

第 39 卷第 7 期 精 细 化 工 Vol.39, No.7
2022 年 7 月 FINE CHEMICALS July 2022

催化与分离提纯技术
双相深共熔溶剂提取的银杏果外种皮

活性成分的分离回收

1
董其惠 1,2 ，吴 蓉 1,2 ，王慧敏 1,2 ，张 文 1,2 ，曹福亮 ，苏二正 1,2*
（1. 南京林业大学 南方现代林业协同创新中心，江苏 南京 210037；2. 南京林业大学 轻工与食品学院
食品科学与工程系，江苏 南京 210037）

摘要：用双相深共熔溶剂（TP-DES）体系同时提取分离银杏果外种皮中亲水性成分萜内酯、黄酮、原花青素和
疏水性成分银杏酚酸，分别利用环氧乙烷-环氧丙烷共聚物（EOPO）和大孔树脂对 TP-DES 中的亲水性和疏水
性活性成分进行回收。萜内酯、黄酮和原花青素用分光光度法测定，银杏酚酸用 HPLC 测定，并根据回收过程
中每一步的活性成分含量计算回收率。采用相对分子质量（简称分子量）为 2650，质量分数为 90%的 EOPO 水
溶液与亲水相 DES 提取液按质量比 4∶6 组成回收体系，对 TP-DES 中萜内酯、黄酮和原花青素进行回收，经
两次形成双相，最终其回收率分别可达 89.44%、53.27%和 29.77%；HYA-502B 树脂静态吸附-解吸回收银杏酚
酸，回收率可达 93.33%。
关键词：深共熔溶剂；环氧乙烷-环氧丙烷共聚物；大孔树脂；回收；银杏果外种皮；分离技术
中图分类号：O629 文献标识码：A 文章编号：1003-5214 (2022) 07-1402-09

Separation and recovery of bioactive ingredients from Ginkgo biloba
exocarp by two-phase extraction with deep eutectic solvents

1,2
1,2
1,2
1,2
1,2
DONG Qihui , WU Rong , WANG Huimin , ZHANG Wen , CAO Fuliang , SU Erzheng 1,2*
(1. Co-innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037,
Jiangsu, China; 2. Department of Food Science and Technology, College of Light Industry and Food Engineering,
Nanjing Forestry University, Nanjing 210037, Jiangsu, China)
Abstract: Hydrophilic terpene lactones, flavonoids, procyanidines and hydrophobic ginkgolic acids were
simultaneously extracted from Ginkgo biloba exocarp by using a two-phase system with deep eutectic
solvents (TP-DES). And the hydrophilic and hydrophobic active components in TP-DES were further
recovered by ethylene oxide-propylene oxide copolymer (EOPO) and macroporous resin, respectively. The
extracted terpene lactones, flavonoids and procyanidins were confirmed by spectrophotometry while
ginkgolic acids by HPLC, and the recovery rate was calculated based on the mass concentration of active
components in each step. It was found that after forming biphasic phase twice the recovery rates of terpene
lactones, flavonoids and procyanidins in TP-DES were 89.44%, 53.27% and 29.77% respectively when
using EOPO aqueous solution with a relative molecular mass (short for molecular mass) of 2650 and a mass
fraction of 90% composed a recovery system with hydrophilic phase DES. Meanwhile, ginkgolic acids
recovery rate reached 93.33% by static adsorption-desorption with HYA-502B resin.
Key words: deep eutectic solvents; ethylene oxide-propylene oxide copolymer; macroporous resin;
recovery; Ginkgo biloba exocarp; separation technology

[1]
银杏（Ginkgo biloba L.）是目前研究最广泛的 药用植物之一 。银杏果组成包括肉质外种皮、骨

收稿日期：2022-01-18; 定用日期：2022-03-11; DOI: 10.13550/j.jxhg.20220066
基金项目：中国博士后科学基金（2016M600417，2017T100373）；江苏省第五期“333 工程”培养资金资助项目（BRA2017458）
作者简介：董其惠（1996—），女，博士生，E-mail：dongqihui0823@njfu.edu.cn。联系人：苏二正（1976—），男，教授，博士生导
师，E-mail：ezhsu@njfu.edu.cn。