Page 109 - 《精细化工》2023年第1期

第 40 卷第 1 期 精 细 化 工 Vol.40, No.1
20 23 年 1 月 FINE CHEMICALS Jan. 2023

中药现代化技术
响应面法优化板栗苞中抑制痢疾杆菌

活性成分的提取工艺

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陈丽楠 1,2 ，彭 飞 1,2,3 ，靳长迎 ，余祚庆 ，杨越冬 1,2,3*
（1. 河北省天然产物活性成分与功能重点实验室，河北 秦皇岛 066004；2. 板栗产业技术教育部工程研
究中心，河北 秦皇岛 066004；3. 河北省板栗产业协同创新中心，河北 秦皇岛 066004）

摘要：为提高板栗废弃物的资源利用率，以板栗苞为原料，探究其抑制痢疾杆菌活性成分的提取工艺。采用吸
光光度法筛选板栗苞抑制痢疾杆菌活性成分的提取溶剂；以抑菌率为响应值，采用单因素实验结合响应面法优
化板栗苞抑菌活性成分的提取工艺；采用二倍稀释法测定板栗苞各提取物的最低抑菌质量浓度（MIC），并采用
吸光光度法研究其对细菌生长曲线的影响。结果表明，板栗苞的乙醇水提取物对痢疾杆菌的抑菌活性最好；板
栗苞抑菌成分的最佳提取工艺为乙醇体积分数 45%，料液比 1∶20（g∶mL），提取温度 50 ℃；在该条件下，
板栗苞提取物抑菌率为 61.27%±2.60%，MIC 值为 3.2 g/L；乙酸乙酯相提取物 MIC 值为 0.4 g/L，抑菌率达 85%
以上。
关键词：板栗苞；提取；抑菌活性；响应面优化；中药现代化技术
中图分类号：TS201.3；R284 文献标识码：A 文章编号：1003-5214 (2023) 01-0101-08

Extraction optimization by response surface methodology of active
ingredients from chestnut burs for Shigella dysenteriae inhibition

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CHEN Li'nan , PENG Fei 1,2,3 , JIN Changying , YU Zuoqing , YANG Yuedong 1,2,3*
（1. Hebei Key Laboratory of Active Components and Functions in Natural Products, Qinhuangdao 066004, Hebei,
China; 2. Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Qinhuangdao 066004,
Hebei, China; 3. Hebei Collaborative Innovation Center of Chestnut Industry, Qinhuangdao 066004, Hebei, China）

Abstract: In order to improve the resource utilization rate of chestnut waste, the extraction process of
active ingredient from chestnut burs against Shigella dysenteriae were explored. The extraction solvent was
screened via spectrophotometry, and the extraction process was optimized by single factor experiment
combined with response surface methodology with bacteriostatic rate as response value. The minimum
inhibitory concentration (MIC) of extracts from chestnut burs were determined by double dilution method,
and its effect on bacterial growth curve was analyzed by absorbance spectrophotometry. The results
demonstrated that the extract from chestnut burs by ethanol and water mixture exhibited the best
antibacterial activity against Shigella dysenteriae, with antibacterial rate of 61.27%±2.60% and MIC value
of 3.2 g/L, under the optimal extraction conditions of ethanol and water mixture (ethanol volume fraction of
45%) as solvent, ratio of material to liquid 1∶20 (g∶mL), extraction temperature 50 ℃. Meanwhile, the
MIC value of ethyl acetate phase extract was 0.4 g/L, and the antibacterial rate was above 85%.
Key words: chestnut burs; extraction; antibacterial activity; response surface optimization; modernization
technology of traditional Chinese medicines

收稿日期：2022-06-05; 定用日期：2022-08-26; DOI: 10.13550/j.jxhg.20220526
基金项目：河北省研究生创新资助项目（CXZZSS2022090）
作者简介：陈丽楠（1998—），女，硕士生，E-mail：chen15534445342@163.com。联系人：杨越冬（1965—），女，博士，教授，E-mail：
yuedongyang@hotmail.com。