Page 80 - 《精细化工》2023年第11期

·2392· 精细化工 FINE CHEMICALS 第 40 卷

及 COL24h 对 NIH/3T3 的促生长作用更明显，并且 biocompatibility of a novel compressed collagen hydrogel scaffold
在培养的过程中，这两组样品的促生长作用保持快 for artificial corneas[J]. Journal of Biomedical Materials Research
Part A, 2014, 102(6): 1782-1787.
速增加的趋势。相比对照 COL，COL24h 更利于细 [5] SUO H, ZHANG J, XU M, et al, Low-temperature 3D printing of
胞生长。胶原蛋白的三螺旋结构是其发挥生物功能 collagen and chitosan composite for tissue engineering[J]. Materials
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的基础。胶原蛋白能够提供细胞结合的位点，从而 [6] LIN K, ZHANG D, MACEDO M H, et al. Advanced collagen-based
有利于细胞的贴壁和繁殖。经冷冻研磨处理后的胶 biomaterials for regenerative biomedicine[J]. Advanced Functional
原蛋白改变了分子间的相互作用力，可能暴露出更 Materials, 2019, 29(3): 1804943.
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多的结合位点，更利于细胞生长和繁殖。 and collagen-based therapeutic materials for skin repair[J]. ACS
Biomaterials Science & Engineering, 2020, 6: 1124-1134.
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Materials, 2020, 13: 786-801.
[9] XU J, ZHENG S, HU X, et al. Advances in the research of bioinks
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skin 3D bioprinting[J]. Polymers, 2020, 12: 1237-1267.
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collagen/apatite composite biomaterials for potential use in bone
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conditions[J]. Meat Science, 2016, 113: 116-123.
*代表 P<0.05；***代表 P<0.001；****代表 P<0.0001 [12] CHEN C, LIU F, YU Z, et al. Improvement in physicochemical
图 9 NIH/3T3 在样品中培养 1~5 d 的活性 properties of collagen casings by glutaraldehyde cross-linking and
Fig. 9 Activity of NIH/3T3 after 1~5 d incubation in samples drying temperature regulating[J]. Food Chemistry, 2020, 318: 1-9.
[13] YANG J, WANG H B, HE L, et al. Reconstituted fibril from
heterogenic collagens-A new method to regulate properties of collagen
gels[J]. Macromolecular Research, 2019, 27(11): 1124-1135.
3 结论 [14] PRASHANT K B, PADMA B D. Isolation, characterization and
valorizable applications of fish scale collagen in food and agriculture
运用冷冻研磨技术，通过改变预冻时间对天然 industries[J]. Biocatalysis and Agricultural Biotechnology, 2016, 7:
牛跟腱胶原蛋白海绵进行粉碎处理，以期实现研磨 234-240.
[15] DAI L, NAN J, TU X, et al. Improved thermostability and
产物的性能调控。SDS-PAGE 和 CD 实验结果发现， cytocompatibility of bacterial cellulose/collagen composite by collagen
冷冻时间分别为 3、6 h 时，有少量小分子条带，而 fibrillogenesis[J]. Cellulose, 2019, 26(11): 6713-6724.
冷冻 24 h 后胶原蛋白的肽链结构保持得更完整。 [16] JOSE F W, BOLESLAV Z, ONDREJ Š, et al. Study of the
shear-thinning effect between polymer nanoparticle surfaces during
AFM 和 SEM 实验证明，冷冻研磨得到的胶原蛋白 shear-induced aggregation[J]. Industrial & Engineering Chemistry
保持体外自组装能力，COL24h 组装得到的胶原蛋 Research, 2021, 60, 29: 10654-10665.
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白纤维直径与 COL 无显著性差异。溶解度实验表
thermally denatured collagen by ultrahigh pressure treatment[J].
明，冷冻研磨可以显著提高胶原蛋白的水溶性 Food Bioprocess Technology, 2020, 13(2): 367-378.
（4 ℃），COL24h 比未经任何处理的天然胶原蛋白 [18] JIANG Y, WANG H B, DENG M X, et al. Effect of ultrasonication
on the fibril-formation and gel properties of collagen from grass carp
（COL）的溶解度提高近 3 倍，这将对胶原蛋白作 skin[J]. Materials Science & Engineering C-Materials for Biological
为医美材料的应用有更好的支撑作用。细胞生物学 Applications, 2016, 59(1): 1038-1046.
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的影响。
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