Page 119 - 《精细化工》2023年第4期
P. 119
す 4 䲠ͦͦ喑ぶ: ็ߌ㘪㏠ㆠٶ᩼ݯ NaYF 4 ńYb,Tm@NaGdF 4 ńYb@PDA/PEI-MnO 2 ⮱ݣิࣷោ㣹ᕔ㘪g805g
2+
2+
ᐼ͚喟³(Mn )ͧ ICP ≸ᓄ⮱ Mn ⮱䉕䛼⊀Ꮣ喑 वᎣ̷⌲⋟হ≄⋑⋟ڞ 2 mL喍V喎喑⩕ᬍⅡΆ䚴⼭䛷
mg/L喠m UPM ͧ UPM 䉕䛼喑mg喠V ͧᒲ≸⋟Ҁ⼜喑 20 Ժ喑≸Ⴧ䄒⏣⋟ౕ 360 nm ั⮱॥ٶᏓ喑ӊᢛ TC
L喠n ͧᒲ≸⋟⮱⼭䛷Ժȡ ⮱䉕䛼⊀Ꮣ-॥ٶᏓᴴ۳ᰟ㏬喍ȡ = 0.0251A – 0.0003喑
2
1.3.2 ㆨ䓴⅔ࡃ⅏䚣≨ᕔ⮱≸Ⴧ R = 0.9995喎ᓄݝ⼭䛷⏣⋟͚ TC ⮱䉕䛼⊀Ꮣ喍ȡ喎喑
В UPM יࡃܳ㼐 H 2O 2 ϔ⩌ O 2 ߍҀ㈨⏣⅔䛼 ӊᢛᐼ喍3喎䃎ツ UPM ⮱䒪㢜䛼喍DL喎喟
⮱㘪߈Გ䃱Фٶ᩼ݯ⮱ㆨ䓴⅔ࡃ⅏䚣≨ᕔᑧᑞȡڤ DL/%= (ȡ 0 V 0 –20ȡV) /m c ×100 喍3喎
Ҁₒ俑ͧ喟ᄳ 3 mg UPM ܳ᪐ౕ 9 mL ⸤䚥⯽㑀۟⋟ ᐼ͚喟ȡ ͧ⼭䛷⏣⋟͚ TC ⮱䉕䛼⊀Ꮣ喑g/L喠V ͧ
喍PB喑10 mmol/L喑pH = 6.5喎͚喑ᄳ⌤व➖䒙⼨ڒ वᎣ⋟⮱Ҁ⼜喑L喠m c ͧ䒪Ҁ UPM ⮱䉕䛼喑g喠V 0
☔Ნ͚Ꭳ㒛λ 25 ĎⅡ⊡͚喑ᒲ⍖Ꮣ⽠ჇऻՌߖ⏣ ͧ݊ TC ⏣⋟Ҁ⼜喑L喠ȡ 0 ͧ݊ TC ⏣⋟͚ TC
⅔З䃝ᒂ⏣⋟͚݊⏣⅔䛼ȡߍڒ 285 ȝL ⊀Ꮣͧ ⮱䉕䛼⊀Ꮣ喑g/Lȡ
32.6 mmol/L H 2 O 2 Ⅱ⏣⋟喍࣌᫆⩕ࣨ⻨ၽⅡ⼭䛷 600 ᄳ̷䔝侹ᓄݝ⮱ UPM/TC 䊲ฝܳ᪐ݝ 1 mL
Ժ喎ҬҀ㈨͚ H 2 O 2 ⮱⊀Ꮣͧ 1 mmol/L喑ౕᔘ䕌ᤲ ̺ह䚥Ꮣ⮱ PB喍10 mmol/L喑pH = 5.0Ƞ6.5 হ 7.4喎
៹̸䃝ᒂ̺हᬣݨ⮱⏣⅔䛼ȡ䄰᪡Ҁ㈨͚ H 2 O 2 ⊀Ꮣ ⏣⋟͚喑ౕ 37 ĎⅡ⊡͚䖬ٶᡜ㢎喑1 h ऻ⻨ᓰ↶⋭
ͧ 0.1Ƞ0.5 mmol/L喑जВ㣤ᓄ UPM-3 יࡃܳ㼐क़ ㆿၽ喑॥ܧ̷⌲⋟ 1 mL喍V 1 喎喑⩕ᬍⅡΆ䚴⼭䛷 20
̺ह⊀Ꮣ H 2 O 2 Ҁ㈨⮱⏣⅔䛼ȡ Ժऻ≸Ⴧڣ͚ TC ⮱䉕䛼⊀Ꮣ喍ȡ 1 喎ȡाㆿၽ͚㶒ߍ
1.3.3 •OH ϔ⩌㘪߈⮱≸Ⴧ 1 mL PB ⏣⋟喑䛺䊲ฝܳ᪐ऻ㐔㐚ౕ 37 Ď̸䛷ᩫ
В TA ͧᣏ䦵ᷭ≸ UPM יࡃ H 2 O 2 ϔ⩌•OH ⮱ TC喑ౕ 1Ƞ2Ƞ4Ƞ6Ƞ12 হ 24 h ᬣ䛺̷ฺ䔝᧺҉喑
㘪߈ȡ⩕ 1 mol/L NaOH Ⅱ⏣⋟䄰㞯 0.268 mmol/L TA ≸ᓄᄦᏁ⏣⋟͚ TC ⮱䉕䛼⊀Ꮣ喍ȡ 2 ~ ȡ n 喎喑ӊᢛᐼ喍4喎
Ⅱ⏣⋟㜠 pH=7.0喑ा 1 mL TA ͚ӊ⁎ߍڒ 0.5 mL 䃎ツ㉜⼜䛷㢜⢴喑㐅ݣ㉜⼜䛷㢜⢴-ᬣ䬡ᰟ㏬ȡ
䉕䛼⊀Ꮣͧ 2 g/L ⮱ UPM-3 ܳ᪐⋟হ 0.5 mL ⊀Ꮣͧ 20 V U u Ă U
㉜⼜䛷㢜⢴/%= 1 n 1 ×100 喍4喎
4 mmol/L H 2 O 2 ⏣⋟喍Ҭ⩕ 0.2 mol/L pH= 5.0 䚸䚥 DL mu c
㑀۟⋟䙺ݣ喎喑䖬ٶᤲ៹ 30 min ऻ⻨ᓰࣨ䮑ㆿၽ喑 ᐼ͚喟V 1 ͧ䛷ᩫ⋟⮱Ҁ⼜喑L喠ȡ n ͧす n ⁎⼭䛷ऻ
ౕ 315 nm ⓭ࣾٶ̸≸䄂⏣⋟⮱㢔ٶٶ䅞ȡܳݘВぶ ⮱䛷ᩫ⋟͚ TC ⮱䉕䛼⊀Ꮣ喑g/Lȡ
Ҁ⼜⮱䚸䚥㑀۟⋟Аᰬ̷䔝≸䄂 H 2 O 2 ⏣⋟হ喍ᝃ喎 1.3.6 Ҁใោ㣹ᕔ㘪⮱≸Ⴧ
UPM-3 ܳ᪐⋟喑ӊᢛٶ䅞⮱ᑧᏓअࡃ䃱ѝ UPM י ᄳ๔㗍ᱳ㣹㣹ᣒ㜠▚㣹ऻ⮱ LB ⋟Ҁദڨ
ࡃ H 2 O 2 ϔ⩌•OH ⮱㘪߈ȡ ധ͚喑ᩫڒᕿ⍖ᥴᎷ喍37 Ď喑200 r/min喎叾ᯄദڨ
1.3.4 ROS ϔ⩌㘪߈⮱≸Ⴧ 18 h Ҭ㏳㣹≨ࡃ喑⩕ LB ⋟Ҁദڨധ⼭䛷≨ࡃ⮱๔
1
DPBF ᭜ O 2 ᠴݯ喑जՌߖ DPBF ⮱䭺㼐⢴Გ 㗍ᱳ㣹ᗙ⋟喑㣤ᓄ㣹⊀Ꮣ㏓ 1.5×10 CFU/mL ⮱ᴴ
8
䃱Фٶ᩼ݯ UPM ౕ NIR ٶ⚔ᄱ̸ϔ⩌ ROS ⮱㘪߈ȡ
۳㣹ᗙ⋟ȡ䛴⩕ᎠᲬ∂䃱Фᵤ৮⮱ោ㣹ᕔ㘪ȡᄳ
ᄳ 2.5 mg UPM-3 䊲ฝܳ᪐ౕ 5 mL 䉕䛼⊀Ꮣͧ
0.4 mg UPȠUPM হ UPM/TC ܳݘܳ᪐ౕ 0.49 mL LB
30 mg/L DPBF ⮱ᬍⅡΆ䚴⏣⋟͚喑䖬ٶᤲ៹ 2 h 䓫
⋟Ҁദڨധ͚喑ा̷䔝ܳ᪐⋟Вࣷ 0.49 mL 䉕䛼⊀
ݝ॥䭱-㙞䭱Ꭰ㶎喑≸Ⴧ⏣⋟ౕ 412 nm ั⮱॥ٶᏓ Ꮣͧ 0.186 g/L ⮱ TC ⏣⋟喍̻ UPM/TC ܳ᪐⋟क़
2
թ喍A 0 喎ȡ⩕ 980 nm NIR ٶ喍ߌ⢴ჳᏓ 2.1 W/cm 喑
TC ⮱䉕䛼⊀ᏓⰥぶ喎͚ܳݘߍڒ 10 ȝL ᴴ۳㣹ᗙ⋟喑
ٶ᪾Ⱑᒱ 10 mm喑⚔ᄱ䌊⻨ 20 cm喎⚔ᄱࣺᏁ⋟喑
Ҭㆿၽ⮱䉕䛼⊀Ꮣͧ 0.8 g/L喍HG/T 3794ÿ2005 ᴴ
20 min ःᵤ喑⻨ᓰࣨ䮑ㆿၽ喑≸Ⴧ̷⌲⋟ౕ
۳͚⮱ោ㣹ݯ䉕䛼⊀Ꮣ喎喑䖬ٶڞദڨ 30 min ऻ喑
412 nm ั⮱॥ٶᏓթ喍A喎喑㐅ݣ A/A 0 -ᬣ䬡ᰟ㏬ȡ
ᄳ⌤व⋟㒛λ 980 nm NIR ٶ̸⚔ᄱ喍ߌ⢴ჳᏓ
DPBF 䭺㼐⢴ᠶᐼ喍2喎䃎ツ喟
2
1.2 W/cm 喑ٶ᪾Ⱑᒱ 10 mm喑⚔ᄱ䌊⻨ 20 cm喎Ꭳ
DPBF 䭺㼐⢴/%=(1–A/A 0 )f100 喍2喎
ᤲ៹ȡౕٶ⚔̺हᬣ䬡喍0Ƞ5 হ 10 min喎ܳݘःܧ
ͧ䃱ѝٶ᩼ݯౕ᠌㏳㣹ᓛ⣜ධ喍ᑞ䚥ᕔȠH 2 O 2
5
6
ᄾ䛼⌤व㣹⋟喑⼭䛷㜠 1×10 ~1×10 CFU/mL喑ᄳ⼭
⊀Ꮣռ倅喎͚⮱ PDT ≨ᕔ喑ౕ॥䭱Ꭰ㶎ऻा̷䔝Ҁ
䛷⋟࠭⊯ጰౕధҀദڨധ喍LB ⋟Ҁദڨധ+⥩㘯
㈨ߍڒ 330 ȝL ⊀Ꮣͧ 16.3 mmol/L ⮱ H 2 O 2 ⮱ PB ⏣
ㆶ喎㶕䲏喑ᩫڒ 37 ĎȠCO 2 ദڨマ͚叾ᯄദڨ 24 hȡ
⋟喍10 mmol/L喑pH = 6.5喎喑♣ऻ䔈㵹ٶ⚔ࣺᏁȡ
В⌨ߍ 10 ȝL ᴴ۳㣹ᗙ⋟⮱ 0.49 mL LB ⋟Ҁദڨധ
1.3.5 㢜➖䉌䒪হ䛷㢜㘪߈⮱≸Ⴧ
ᵤ৮҉ͧ⾧⮪ᄦ⚔㏱喑ᄦ℁㻯ᄌऱ㏱㏳㣹⮱⩌䪬⟣
ᄳ 2 mg UPM-3 ܳ᪐ౕ 1 mL 䉕䛼⊀Ꮣͧ 0.8 g/L
ۢ喑ВᎠ㵹侹 3 ಄Წ⮱㣹㥪䃎⮱Ꭰթ䃎ツ㏳
TC ⮱ᬍⅡΆ䚴⏣⋟͚喑䖬ٶᤲ៹ 24 h Ⴙ TC ⮱䉌
㣹ႅ≨⢴ȡ๔㗍ᱳ㣹⮱ႅ≨⢴ᠶᐼ喍5喎䃎ツ喟
䒪ȡ⩕ 1 mL ᬍⅡΆ䚴≄⋑ㆿၽ 1 ⁎喑ბ⍖ⱌ⾧✒
㏳㣹ႅ≨⢴/% = N(t)/N(t 0 ) × 100 喍5喎
6 h ऻᓄݝ TC 䉌䒪⮱㏠ㆠٶ᩼ݯ喑䃝ͧ UPM/TCȡ
