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第 35 卷第 7 期 精 细 化 工 Vol.35, No.7
201 8 年 7 月 FINE CHEMICALS July 2018

中药现代化技术
铁棒锤中 3-乙酰乌头碱和宋果灵抗肿瘤活性

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孙建瑞，邱智军，王大红，张 彬，原江锋
（河南科技大学 食品与生物工程学院，洛阳市微生物发酵工程技术研究中心，河南 洛阳 471023）

摘要：从铁棒锤总生物碱中提取分离得到了 3-乙酰乌头碱和宋果灵，采用四唑盐（MTT）比色法考察了药物的
细胞毒性，电子显微镜及激光共聚焦显微镜观察了细胞形态，流式细胞术分析了细胞凋亡和细胞周期。抗肿瘤
活性研究表明：随着 3-乙酰乌头碱和宋果灵浓度的增加，抗肿瘤效果逐步增强，当质量浓度均为 64 mg/L 时，
对 HepG2 细胞的抑制率分别最高达到 93.56%和 98.48%。药物作用于人肝癌细胞后，能明显抑制 HepG2 细胞的
生长，并且使细胞发生早期凋亡和晚期凋亡的比率增大，质量浓度为 100 mg/L 3-乙酰乌头碱使早期细胞凋亡率
上升至 58.32%，晚期凋亡率增至 13.81%；质量浓度为 120 mg/L 宋果灵使早期细胞凋亡率上升至 41.02%，晚期
凋亡率增至 28.78%。随处理时间的延长 G1 期细胞逐渐增多，G2 期和 S 期细胞逐渐减少。
关键词：铁棒锤；3-乙酰乌头碱；宋果灵；HepG2；抗肿瘤活性；中药现代化技术
中图分类号：R979.1 文献标识码：A 文章编号：1003-5214 (2018) 07-1163-07

Anti-tumor Activity of 3-Acetylaconitine and Songorine from
Aconitum szechenyianum Gay.

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SUN Jian-rui, QIU Zhi-jun, WANG Da-hong, ZHANG Bin, YUAN Jiang-feng
（College of Food and Bioengineering, Henan University of Science and Technology, Luoyang Engineering and Technology
Research Center of Microbial Fermentation, Luoyang 471023, Henan, China）

Abstract: 3-Acetylaconitine and songorine were extracted and separated from the total alkaloids of
Aconitum szechenyianum Gay., and cytotoxicity of drugs were studied by MTT method using human liver
cancer cells (HepG2). Morphological changes of carcinoma cells were also investigated by using inverted
microscope and laser scanning confocal microscope (LSCM). Cell apoptosis and cell cycle were analyzed
by flow cytometry. The antitumor experiments showed that the antitumor effects were gradually enhanced
with the increase of the concentration of 3-acetylaconitine and songorine, and the highest level was 93.56%
and 98.48%, respectively, when mass concentration were all 64 mg/L. The growth of HepG2 cells was
inhibited significantly after the drugs acted on HepG2, and the ratio of cell early apoptosis and late
apoptosis increased. 3-Acetylaconitine increased early cell apoptosis rate to 58.32% and late apoptosis rate
to 13.81%, when mass concentration was 100 mg/L, while songorine made early cell apoptosis rate and late
apoptosis rate increased to 41.02% and 28.78%,when mass concentration was 120 mg/L. With the
prolonging of treatment time, the G1 phase cells gradually increased, but G2 and S phase cells gradually
decreased.
Key words: Aconitum szechenyianum Gay.; 3-acetylaconitine; songorine; HepG2; antitumor activity;
modernization technology of traditional Chinese medicines
Foundation items: National Natural Science Foundation of China (31401672 and U1404307); Science and
Technology Project of Henan Province (162102110056); Key Science Research Project of Henan Higher
Education Institutions (17B180003)

收稿日期：2017-07-29; 定用日期：2017-11-13; DOI: 10.13550/j.jxhg.20170615
基金项目：国家自然科学基金资助项目（31401672, U1404307）；河南省科技项目（162102110056）；河南省高等学校重点科研项目
（17B180003）
作者简介：孙建瑞（1987—），男，讲师，博士。联系人：原江锋（1977—），女，副教授，电话：18638477899，E-mail：jiangfengyuan@163.com。