Page 121 - 201902

第 2 期 张宇靖，等: 高纯竹叶碳苷黄酮复合纳米材料的制备及结构 ·287·

提高至 52.63 J/g，这表明 γ-CD 的包裹提高了纳米材 2005, 43(5): 783-792.
[3] Nirmala C, Bisht M S, Bajwa H K, et al. Bamboo: A rich source of
料的熔融温度，增加了热焓，提高了纳米材料的稳 natural antioxidants and its applications in the food and
定性。热重分析结果显示， H -BLFnd 与 H -BLFnp 在 pharmaceutical industry[J]. Trends in Food Science & Technology,
2018, 77: 91-99.
较低温度（45~100 ℃）时出现由失水引起的失重， [4] Hu C, Zhang Y, Kitts D D. Evaluation of antioxidant and prooxidant
与 DSC 结果一致。 H -BLFnd 在 246 ℃开始分解， activities of bamboo phyllostachys nigra var henonis leaf extract in
vitro[J]. Journal of Agricultural and Food Chemistry, 2000, 48(8):
H -BLFnp 在 324 ℃时开始分解，分解温度的升高可 3170-3176.
能是由于 γ-CD 与 H -BLFnd 的相互作用引起的。 [5] Zhang Ying (张英), Shen Jianfu (沈建福), Yu Zhuoyu (俞卓裕),
et al. Primary studies on bamboo-leaf-flavonoids used as anti-aging
factor for skin protection[J]. Chemistry and Industry of Forest
Products (林产化学与工业), 2004, 24(1): 95-100.
[6] Poonam S, Lalit M, Santosh S, et al. Bamboo shoots: A novel source
of nutrition and medicine[J]. Critical Reviews in Food Science and
Nutrition, 2011, 5(53): 517-534.
[7] Wang Wenyuan (王文渊), Cai Min (蔡民), Li Yuting (李玉婷),
et al. Study of efficacy of skin care cream formulated with flavonoids
from bamboo leaves[J]. China Surfactant Detergent & Cosmetics(日
用化学工业), 2011, 41(6): 430-433.
[8] Zielińska D, Zieliński H. Antioxidant activity of flavone C-glucosides
determined by updated analytical strategies[J]. Food Chemistry,
2011, 124(2): 672-678.
[9] Gong Jinyan (龚金炎), Wu Xiaoqin (吴晓琴), Zhang Ying (张英),
et al. Advanced research of flavonoid C-glycosides and their

pharmacological effects[J]. Natural Product Research and
图 6 H -BLFnd (a) 和 H -BLFnp (b) 的 DSC 曲线 Development (天然产物研究与开发), 2010, 22(3): 525-530.
Fig. 6 DSC curves of H -BLFnd (a) and H -BLFnp (b) [10] Wang Nan (王楠), Tang Weimin (唐伟敏), Chu Bingquan (楚秉泉) ,
et al. Whitening efficacy of bamboo leaf flavonoids nanoparticles
2.3 皮肤和黏膜刺激性评价 based on B16 melanoma cell evaluation system[J]. Fine Chemicals
(精细化工), 2016, 33(12): 1375-1380.
H -BLFnp 对小鼠的皮肤刺激性实验中，每天每 [11] Abdelwahed W, Degobert G, Stainmesse S, et al. Freeze-drying of
nanoparticles: Formulation, process and storage considerations[J].
只动物皮肤刺激反应积分均值为 0，属于无刺激性。 Advanced Drug Delivery Reviews, 2006, 58(15): 1688-1713.
H -BLFnp 对小鼠的急性眼刺激性实验中，眼膜、虹 [12] Beirowski J, Inghelbrecht S, Arien A, et al. Freeze drying of
nanosuspensions, 2: The role of the critical formulation temperature
膜、结膜的刺激性最高积分均值均为 0。皮肤刺激性 on stability of drug nanosuspensions and its practical implication on
和眼刺激性实验表明，在皮肤外用条件下， H -BLFnp process design[J]. Journal of Pharmaceutical Sciences, 2011,
100(10): 4471-4481.
对皮肤及黏膜未产生刺激作用，安全性高。 [13] Beirowski J, Inghelbrecht S, Arien A, et al. Freeze-drying of
nanosuspensions 1: Freezing rate versus formulation design as
3 结论 critical factors to preserve the original particle size distribution[J].
Journal of Pharmaceutical Sciences, 2011, 100(5): 1958-1968.
[14] Beirowski J, Inghelbrecht S, Arien A, et al. Freeze-drying of
与前期发表的竹叶黄酮纳米混悬液相比，本文 nanosuspensions, part 3: Investigation of factors compromising
storage stability of highly concentrated drug nanosuspensions[J].
制备了 H -BLFnp，将竹叶黄酮中 4 个碳苷的总质量 Journal of Pharmaceutical Sciences, 2012, 101(1): 354-362.
分数从 25%提高到 90%以上，并且黄酮的包埋率从 [15] Zelenková T, Onnainty R, Granero G E, et al. Use of microreactors
and freeze-drying in the manufacturing process of chitosan coated
原先的 40%提高到 90%以上。采用芫根阴离子多糖 PCL nanoparticles[J]. European Journal of Pharmaceutical Sciences,
（半乳糖醛酸质量分数>67%）为壁材，在改善制品 2018, 119(1): 135-146.
[16] Valle E. Cyclodextrins and their uses: A review[J]. Process
色泽的同时，提高了亲水性和皮肤的附着性，适用 Biochemistry, 2004, 39(9): 1033-1046.
于水感型产品。此材料有助于解决植物黄酮在化妆 [17] Zhang W, Ma J, Ren H, et al. Cyclodextrin inclusion technique and
its application in food industry[J]. Food Industry, 2014, 35(9): 256-
品应用中普遍存在的溶解度较低、产品色泽差、不 260.
易被皮肤吸收等问题，同时提高了有效成分的稳定 [18] Tang Weimin (唐伟敏). Comparative studies on chemical structure
and anti-fatigue effect of polysaccharides both from Turnip (Brassica
性、改善了分散性，促进其在皮肤表层的渗透和吸 rapa L.) and Maca (Lepidium meyenii Walp.) [D]. Hangzhou:
收，改进了护肤功效。在 γ-CD 的包裹和保护下，通 Zhejiang University (浙江大学), 2017.
[19] Ruttala H B, Ramasamy T, Gupta B, et al. Multiple polysaccharide-
过冷冻干燥将纳米混悬液制备成粉末化制剂，便于 drug complex-loaded liposomes: A unique strategy in drug loading
and cancer targeting[J]. Carbohydrate Polymers, 2017, 173: 57-66.
储存、运输和精确添加，在日用化妆品，尤其是水
[20] General Administration of Food and Drug Administration of the
感型产品中具有良好的应用前景。 Peoples Republic of China (中华人民共和国食品药品监督管理总
局). Safety technical specifications for cosmetics (2015 edition) [S].
参考文献： Beijing: Peoples Medical Publishing House (人民卫生出版社),
2015: 490-495.
[1] Zhang Ying ( 张英 ). Natural functional extract of bamboo [21] Rogosic M, Mencer H J, Gomzi Z. Polydispersity index and
leaves—bamboo leaf anthoxanthin[J]. China Food Additives (中国食 molecular weight distributions of polymers[J]. European Polymer
品添加剂), 2002, 1(3): 54-58. Journal, 1996, 32(11): 1337-1344.
[2] Lu B, Wu X, Tie X, et al. Toxicology and safety of anti-oxidant of [22] Liu Y, Liu D, Zhu L, et al. Temperature-dependent structure stability
bamboo leaves. Part 1: Acute and subchronic toxicity studies on and in vitro release of chitosan-coated curcumin liposome[J]. Food
anti-oxidant of bamboo leaves[J]. Food and Chemical Toxicology, Research International, 2015, 74: 97-105.