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·670·                             精细化工   FINE CHEMICALS                                  第 36 卷

                 从图 9 可看出,洗脱流速越大,峰拖尾现象越                            志 ).  Necessity  of  protection  of  Empetrum nigrum germplasm
                                                                   resource in the Greater Xing'an Mountains, Inner Mongolia[J]. Inner
            明显,所需要的洗脱溶剂就越多;而洗脱流速越小,                                Mongolia Forestry Investigation and Design (内蒙古林业调查设计),
            成分越集中,所需洗脱溶剂越少,但所需要的时间                                 2010, 33(6): 92-93.
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            选择 3 mL/min 为洗脱流速。                                 [5]   Kim K C, Kang K A, Zhang R, et al. Risk reduction of ethyl acetate
                                                                   fraction  of  Empetrum nigrum var. japonicum via  antioxidant
            2.4.5    验证实验
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                 根据上述探究好的条件(上样液质量浓度                                of Toxicology & Environmental Health, 2009, 72(21/22): 1499-1508.
            5 g/L,最大上样量为 400 mL,洗脱溶剂为体积分数                      [6]   Li Ruiming (李瑞明), Wang Zhenyue (王振月), Kang Yihua (康毅
                                                                   华), et al. Pharmacognostical studies on Empetrum nigrum[J].Central
            95%的乙醇,洗脱体积 5 BV,洗脱流速 3 mL/min)                        South Pharmacy (中南药学), 2005, 3(3): 175-177
            对东北岩高兰进行纯化,纯化后的东北岩高兰的 HPLC                         [7]   Zhang Yulian (张玉莲), Mei Renqiang (梅任强), Liu Xi (刘熙), et
                                                                   al.  Chemical  constituents  of  Empetrum nigrum var.  japonicum[J].
            图见图 10。                                                Chinese  Traditional  and  Herbal  Drugs  (中草药),  2014,  45(16):
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                                                                   Chalcones  from  Empetrum nigrum var. japonicum[J].  Chinese
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                                                                   Chemical  constituents  of  Empetrum nigrum L.  var.  japonicum[J].
                                                                   Journal of Qiqihar University(Natural Science Edition) (齐齐哈尔大
                                                                   学学报:  自然科学版), 2016, 32(4): 54-55.
                                                               [10]  Song Manling (宋漫玲), Tao Junyu (陶俊宇), Yang Jian (杨剑), et
                                                                   al.  Study  on  separation  and  purification  of  total  flavonoids  from
                                                                   Polygonum hydropiper  Linn.  by  macroporous  resin  [J].Chinese
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                                                               [11]  Zhao Jiahong (赵家洪), Yan Xin (焉鑫), Wang Biying (王碧莹), et

                    图 10    纯化后的东北岩高兰 HPLC 图                       al.  Preliminary  enrichment  and  separation  of  gallic  acid  from
            Fig.  10    Purified  HPLC  spectrum  of  Empetrum nigrum  L.  var.   Chebulae Fructus by macroporous resins[J]. Fine Chemicls (精细化
                   japonicum.                                      工), 2018, 35(4): 597-602.
                                                               [12]  Yan  Youyu  (鄢又玉), Xia Ting  (夏婷),  Zhang  Yu  (张育),  et al.
                 用面积归一化法计算图 1 和图 10 可得:东北岩                         Systematic investigation on AlCl 3 colorimetric determination of total
                                                                   flavonoids from pyracantha fortuneana fruit[J]. Food Research and
            高兰总黄酮纯化前的质量分数为 49.16%,纯化后的                             Development (食品研究与开发), 2015, 36(5): 89-94.
            质量分数提高到 89.59%。                                    [13]  Ye Chun (叶春), Xu Lijuan (徐丽娟), Yang Genqin (杨根勤), et al.
                                                                   Determination  and  identification  of  flavonoids  in  leaves  of
            3   结论                                                 houttuynia cordata thumb[J].Food Research and Development (食品
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                 通过对比 8 种型号的大孔吸附树脂的吸附率和                            斌),  et al.  Optimization  of  enrichment  and  purification  of  total
            解吸率,最终确定 AB-8 型大孔吸附树脂作为纯化                              flavonoids  from  pine  needles  of  Cedrus  deodara  by  macroporous
                                                                   adsorption  resin[J].Chinese  Traditional  Patent  Medicine  (中成药),
            东北岩高兰总黄酮的最佳吸附剂。动态吸附和解吸                                 2017, 39(2): 308-311.
            实验优化工艺条件为:上样液质量浓度为 5 g/L,最大                        [15]  Fan Qin (樊秦), Xia Pengfei (夏鹏飞), Peng Xuejing (彭雪晶), et al.
                                                                   Enrichment  of  atractylenolide  I  and  atractylenolide  Ⅱ   from
            上样量为 400  mL,体积分数为 95%的乙醇洗脱成                           Codonopsispilosula  with  macroporous  resin[J].  Natural  Product
            分,洗脱体积 5 BV,洗脱流速 3 mL/min。在最佳条                         Research  and  Development  (天然产物研究与开发),  2017,  29(9):
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            件下,东北岩高兰总黄酮的质量分数由纯化前的                              [16]  Liu Qingqing (刘清清), Yan Yizhu (阎怡竹), Yang Haixia (杨海霞),
            49.16%提高到纯化后的 89.59%,实现了对东北岩高                          et al.  The  thermodynamic  properties  in  the  process  of  removing
                                                                   pyrethroid  residue  from  honey  using  macroporous  adsorption
            兰总黄酮纯化的目的,工艺基本稳定可行,纯化效                                 resins[J].  Food  and  Fermentation  Industries  (食品与发酵工业),
            果好。为东北岩高兰总黄酮的富集和纯化提供了科                                 2016, 42(12): 57-61.
                                                               [17]  Dong Haohao (董浩浩), Li Congju (李从举), Liu Tingyue (刘廷岳).
            学依据,为进一步开发利用东北岩高兰奠定了基础。
                                                                   et al.  Kinetic  and  thermodynamic  studies  on  absorption  of  anion
                                                                   exchange  fiber  in  baicalin[J].Journal  of  Southwest  China  Normal
            参考文献:                                                  Universit (Natural Science Editiony) (西南师范大学学报:自然科学
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                 阳), et al. Chemical constituents of ethyl acetate extract of Empetrum   [18]  Zhang Shoujun (张守军), Wang Rongshen (王荣申), Lu Yan (卢燕),
                 nigrum var. japonicum  and  their  effect  on  alcoholic  fatty  liver  of   et al. Thermodynamic and kinetic characteristics of static adsorption
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                 japonicum extract suppresses γ-ray radiation-induced cell damage via   [19]  Yang Jinyan (杨金燕), Guo Runqin (郭润勤), Xu Qing (徐清), et al.
                 inhibition  of  oxidative  stress[J].  American  Journal  of  Chinese   Enrichment  and  purification  of  total  flavonoids  from  Emilia
                 Medicine, 2011, 39(1): 161-170.                   sonchifolia by macroporous adsorption resin[J]. Journal of Chinese
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