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第 4 期 王 艺，等: 滇黄精多糖的结构及对葡萄糖苷酶的抑制作用 ·719·

pharmacological applications of Polygonatum sibiricum
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图 7 阿卡波糖对 α-葡萄糖苷酶的抑制效应 2018, 2018(5): 1219367.
Fig. 7 Inhibitory effect of acarbose on α-glucosidase [7] Feng Jing (冯静), Zhang Feiran (张斐然), Yang Xiaohua (杨晓华),et
al. Phytochemicals of three kinds of Se-enriched compositae flowers
表 1 滇黄精多糖抑制-葡萄糖苷酶量效关系的数学模型 and their effects on activity of α-glucosidase[J/OL].Molecular Plant
Table 1 Mathematical model of dose-effect relationship of Breeding( 分子植物育 种 ):1-14[2019-01-23].http://kns.cnki.
Polygonatum kingjanttm polysaccharides against net/kcms/detail/46.1068.S.20180724.0955.006. html.
α- glucosidase [8] Hou Shuangju (侯双菊). Study on the extracting technology of
2
样品种类 拟合方程 R IC 50/(g/L) Polygonatum polysaccharides and the decolorization of the extract by
resin adsorption[D]. Hefei: Hefei University of Technology (合肥工
2
3
滇黄精多糖 y =0.2653x +2.6277x + 0.9881 4.5720 业大学), 2006: 25.
4.6136x0.67
[9] Yang H, Wu Y, Gan C, et al. Characterization and antioxidant activity
2
6
阿卡波糖 y=6×10 x +28978 x+0.9588 0.9895 0.0013
of a novel polysaccharide from Pholidota chinensis Lindl[J].
注：拟合方程中，x 代表样品质量浓度（g/L），y 代表 α- Carbohydrate Polymers, 2016, 138(22): 327-334.
葡萄糖苷酶活性抑制率（%）。 [10] Rostami H, Gharibzahedi S M. Microwave-assisted extraction of
jujube polysaccharide: optimization, purification and functional
characterization[J]. Carbohydrate Polymers, 2016, 143(3): 100-107.
3 结论 [11] Yang J, Zhang H, Cao X, et al. Enzymatic water extraction of
polysaccharides from Epimedium brevicornu and their antioxidant
activity and protective effect against DNA damage[J]. Journal of
本研究从滇黄精中分离制备了活性多糖，分析
Food Biochemistry, 2017, 41(1): 1-12.
了滇黄精多糖的结构，探讨了多糖对 α-葡萄糖苷酶、 [12] Gangwar M, Gautam M K, Sharma A K，et al. Antioxidant capacity
α-淀粉酶活力的抑制效应。FTIR 分析发现，滇黄精 and radical scavenging effect of polyphenol rich mallotus
philippenensis fruit extract on human erythrocytes: an in vitro
多糖中具有吡喃糖苷键，可能是酸性多糖。CD 分析 study[J]. Scientific World Journal, 2014, 2014(1): 1-12.
表明，多糖在 210 nm 波长处有明显的正 cotton 效应。 [13] Šimková D, Lachman J, Hamouz K, et al. Effect of cultivar, location
and year on total starch, amylose, phosphorus content and starch
SEM 观察发现，多糖主要包括多网孔片状结构和多
grain size of high starch potato cultivars for food and industrial
分支链聚集结构 2 种微观形貌。TGA 结果显示，多 processing[J]. Food Chemistry, 2013, 141(4): 3872-3880.
糖在 248~350 ℃失重率最大，在 30~200 ℃时次之， [14] Zhang H F, Niu L L, Yang X H, et al. Analysis of water-soluble
polysaccharides in an edible medicinal plant Epimedium: method
在 400~700 ℃最小。滇黄精多糖对 α-葡萄糖苷酶具
development, validation, and application[J]. Journal of AOAC
有一定的抑制效应，但是对 α-淀粉酶没有明显的抑 International, 2014, 97(3): 787-790.
制作用。本研究为滇黄精多糖的开发利用以及降血 [15] Zhu D Y, Ma Y L, Wang C H, et al. Insights into physicochemical
and functional properties of polysaccharides sequentially extracted
糖药物的研制提供了参考依据。今后可采用动物实 from onion (Allium cepa L. )[J]. International Journal of Biological
验等进一步探讨滇黄精多糖对糖尿病的防治效果与 Macromolecules, 2017, 105(7): 1192-1121.
作用机制。 [16] Wei C, He P, He L, et al. Structure characterization and biological
activities of a pectic polysaccharide from cupule of Castanea
致谢：西安建筑科技大学文堪协助进行傅里叶 henryi[J]. International Journal of Biological Macromolecules, 2018,
变换红外光谱分析，陕西师范大学潘振东在实验技 109(12): 65-75.
[17] Sun Y, Hou S, Song S, et al. Impact of acidic, water and alkaline
术方面提供帮助。
extraction on structural features, antioxidant activities of Laminaria
japonica polysaccharides[J]. International Journal of Biological
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Macromolecules, 2018, 112(2): 985-995.
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