Page 120 - 《精细化工》2020年第11期

第 37 卷第 11 期 精 细 化 工 Vol.37, No.11
2020 年 11 月 FINE CHEMICALS Nov. 2020

功能材料
柠檬提取液生物合成纳米硒及其抗氧化性

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苏 文，杨 辉
（陕西科技大学 食品与生物工程学院，陕西 西安 710021）

摘要：以柠檬提取液为原料生物合成纳米硒，借助 UV-Vis 技术跟踪纳米硒的形成过程，采用 XRD 对纳米硒化
学组成和结构进行了表征，通过 SEM 观察其表面形貌，用粒径仪测定了纳米粒径，利用 FTIR 分析了纳米硒表
面与生物分子的化学作用，并对制备的纳米硒清除超氧阴离子自由基、羟基自由基和 1,1-二苯基-2-三硝基苯肼
（DPPH）自由基的能力进行了测试。结果表明，当 pH=10 时，柠檬提取液料液比为〔1∶5 (g∶mL)〕可还原
50 mmol/L 的亚硒酸生成无定形态纳米硒，反应时间不同，所得纳米硒粒径也不同，反应 1 h 时合成纳米硒粒径
介于 78.8~122.4 nm、反应 2 h 时粒径介于 164.2~295.3 nm，呈球形，分散性较好；柠檬提取液既是合成纳米硒
的还原剂又是稳定剂，其生物分子与纳米硒表面的化学作用是纳米硒稳定存在的基础；纳米硒的抗氧化活性与
质量浓度呈正相关，抗氧化性顺序为：V C >小尺寸纳米硒>大尺寸纳米硒。
关键词：柠檬提取液；纳米硒；抗氧化性；功能材料
中图分类号：O613.52; TB383 文献标识码：A 文章编号：1003-5214 (2020) 11-2266-07

Biosynthesis of selenium nanoparticles from lemon
extract and its antioxidant activity

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SU Wen, YANG Hui
（School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, Shaanxi, China）

Abstract: Selenium nanoparticles were biosynthesized using lemon extract as raw materials. The
formation process of selenium nanoparticles was tracked by UV-Vis technology. The chemical
composition and structure of selenium nanoparticles were characterized by XRD, the surface morphology
was observed by SEM, and the particle size was measured by particle size analyzer. The chemical
interaction between the surface of selenium nanoparticles and biomolecules was analyzed by FTIR. The
scavenging abilities of the prepared selenium nanoparticles on superoxide anion free radical, hydroxyl
free radical and 1,1-diphenyl-2- trinitrophenylhydrazine (DPPH) free radical were tested. It was found
that at pH=10, the lemon extract [ratio of material to solvent of 1∶5 (g∶mL)] could reduce 50 mmol/L
selenite to produce amorphous selenium nanoparticles. The reaction time was different, and the size of
selenium nanoparticles was also different. The particle size of selenium nanoparticles was 78.8~122.4 nm
for 1 h and 164.2~295.3 nm for 2 h. The selenium nanoparticles were spherical and had good dispersion.
Lemon extract was a reducing agent and a stabilizer for the synthesis of selenium nanoparticles, and the
chemical interaction between its biomolecules and the surface of selenium nanoparicles was the basis for
the stable existence of selenium nanoparticles. The antioxidant activity of selenium nanoparticles was
positively correlated with its mass concentration. The order of antioxidant activity was: V C> small-sized
selenium nanoparticles> large-sized selenium nanoparticles.
Key words: lemon extract; selenium nanoparticles; antioxidant property; functional materials

硒是人体必需的微量元素 [1-2] ，作为抗氧化剂 无毒、无副作用 [3-4] 。但硒添加剂量和毒性剂量之

收稿日期：2020-05-31; 定用日期：2020-07-20; DOI: 10.13550/j.jxhg.20200453
基金项目：陕西省科技成果转移推广项目（2017CG-003）
作者简介：苏 文（1990—），女，博士生，E-mail：1026262363@qq.com。联系人：杨 辉（1960—），男，教授，E-mail：yangh@sust.edu.cn。