Page 181 - 《精细化工》2020年第6期

第 6 期 谢桂杰，等: 固体脂质对辅酶 Q10 脂质纳米囊透皮性能的影响 ·1247·

透皮机制。 进 CoQ10 的透皮吸收。在之后的研究中可进一步研
为了更直观地了解 CoQ10 在皮肤中的分布，采 究固体脂质的添加对皮肤角质层结构的影响，进一
用激光共聚焦对其进行了表征，结果如图 7 所示。 步明确 CoQ10-脂质纳米囊的透皮机制。

参考文献：
[1] USAMA M A, NERMIN A, AHMED Z, et al. The antioxidant status
of coenzyme Q10 and vitamin E in children with type 1 diabetes[J].
Jornal de Pediatria, 2019, 95(2): 224-230.
[2] LI L, DU J K , LIAN Y R, et al. Protective effects of coenzyme Q10
against hydrogen peroxide-induced oxidative stress in PC 12 Cell: The
role of Nrf 2 and antioxidant enzymes[J]. Cellular and Molecular
Neurobiology, 2016, 36(1): 103-111.
[3] LONE Y, BHIDE M, KOIRI R K. Amelioratory effect of coenzyme
Q10 on potential human carcinogen microcystin-LR induced toxicity
in mice[J]. Food and Chemical Toxicology, 2017, 102: 176-185.
[4] LI J, MAO T H, YUE C, et al. Coenzyme Q10 protects astrocytes
from ROS-induced damage through inhibition of mitochondria-
mediated cell death pathway[J]. International Journal of Biological
Sciences, 2015, 11(1): 59-66.
[5] HSEU Y C, HO Y G, MATHEW D C, et al. The in vitro and in vivo
depigmenting activity of coenzyme Q10 through the down-regulation
of α-MSH signaling pathways and induction of Nrf 2/ARE-mediated
antioxidant genes in UVA-irradiated skin keratinocytes[J]. Biochemical
Pharmacology, 2019, 164: 299-310.
[6] LOHAN S B, BAUERSACHS S, AHLBERG S, et al. Ultra-small
lipid nanoparticles promote the penetration of coenzyme Q10 in skin
cells and counteract oxidative stress[J]. European Journal of
Pharmaceutics and Biopharmaceutics, 2015, 89: 201-207.
[7] FRANCESCA B, ELISABETTA D, CARMELO P, et al. Nanostructured
lipid carriers loaded with CoQ10: Effect on human dermal fibroblasts
under normal and UVA-mediated oxidative conditions[J]. International
Journal of Pharmaceutics, 2013, 455(1/2): 348-356.

A—CoQ10 油溶液；B—CoQ10-CP-0；C—CoQ10-CP-50%；D— [8] NAYAK K, KATIYAR S S, KUSHWAH V, et al. Coenzyme Q10 and
CoQ10-CP-70%；E—CoQ10-CP-95% retinaldehyde co-loaded nanostructured lipid carriers for efficacy
图 7 不同样品处理后的猪皮截面激光共聚焦图 evaluation in wrinkles[J]. Journal of Drug Targeting, 2018, 26(4):
Fig. 7 CLSM images of skin treated with different samples 333-344.
[9] DRAGICEVIC N, KRAJISNIK D, MILIC J, et al. Development of
由图 7 可见，CoQ10 油溶液处理过的猪皮仅在 hydrophilic gels containing coenzyme Q10-loaded liposomes:
表皮层观察到了微弱的荧光，而 CoQ10-脂质纳米囊 Characterization, stability and rheology measurements[J]. Drug
Development and Industrial Pharmacy, 2019, 45(1): 43-54.
处理过的猪皮在表皮和真皮层均观察到了较明显的
[10] KACI M, BELHAFFEF A, MEZIANE S, et al. Nanoemulsions and
荧光，表明 CoQ10 透过表皮到达了真皮层；此外， topical creams for the safe and effective delivery of lipophilic
经过 CoQ10-CP-70%、CoQ10-CP-95%处理后的猪皮 antioxidant coenzyme Q10[J]. Colloids and Surfaces B: Biointerfaces,
2018, 167: 165-175.
表皮和真皮层的荧光强度明显高于 CoQ10-CP-0、
[11] RUDI W, JURGEN Z, TANJA S. Increase of bioavailability of
CoQ10-CP-50%，这与测得的 CoQ10 在皮肤各层中 coenzyme Q(10) and vitamin E[J]. Journal of Medicinal Food, 2007,
的含量一致。 10(4): 731-734.
[12] FANG Q, MA C L, XIA Q. Preparation and evaluation of trans-
3 结论 resveratrol nanosolve[J]. Integrated Ferroelectrics, 2016, 169(1): 7-14.
[13] XIA N (夏楠). Preparation and evaluation of anti-aging lipid
nanocapsules[D]. Nanjing: Dongnan University (东南大学), 2018.
采用高压均质法制备得到了不同质量分数固体
[14] FANG Q (方巧). Preparation and evaluation of polyphenols loaded
脂质的 CoQ10-脂质纳米囊，并考察了固体脂质对 micro/nanocarriers[D]. Nanjing: Dongnan University (东南大学), 2016.
CoQ10-脂质纳米囊的稳定性及透皮性能的影响。结 [15] XING T K (邢廷康). Preparation, characterization and stability of
果表明，固体脂质的添加对 CoQ10-脂质纳米囊的粒 phenylresorcinollipid nanocapsules[D]. Nanjing: Dongnan University (东
南大学), 2016.
径及稳定性无明显影响；此外，DSC、闭合效应及 [16] JULIA C S, NUTTAKORN B, MAGDALENA H, et al. Ultra-small
体外透皮实验表明，CoQ10-CP-70%、CoQ10-CP- NLC for improved dermal delivery of coenzyme Q10[J]. International
95%能够显著减少皮肤表面水分的蒸发，更好地促 Journal of Pharmaceutics, 2013, 447(1/2): 213-217.