Page 140 - 《精细化工》2021年第1期

第 38 卷第 1 期 精 细 化 工 Vol.38, No.1
202 1 年 1 月 FINE CHEMICALS Jan. 2021

医药与日化原料
绿色合成桑叶银纳米粒及其抗菌抗癌活性

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王春莉，陈忠琴，徐蕾蕾，陈海霞
（天津大学 药物科学与技术学院，天津 300072）

摘要：为了更好地开发出具有良好物理化学及生物活性的银纳米粒，利用桑叶水提取物，通过绿色方法制得桑
叶银纳米粒。以 AgNO 3 浓度、反应温度、桑叶水提物与 AgNO 3 溶液体积比、pH 以及反应时间为影响因素，优
化桑叶银纳米粒最佳合成条件；通过 UV-Vis、SEM 及 FTIR 等对产物进行结构表征；通过测定抑菌圈、最小抑
菌浓度和细胞毒实验评价其抗菌及抗癌活性。结果显示，最佳制备条件为：AgNO 3 浓度 5 mmol/L、反应温度 35 ℃、
桑叶水提液与 AgNO 3 溶液体积比 1∶5、反应体系 pH 11.0 及反应时间 6 h。在此条件下制备的桑叶银纳米粒为
大小均一的球形，平均粒径(48.78 ± 0.39) nm，电位(–27.8 ± 2.00) mV；相比于桑叶水提物，桑叶银纳米粒对大
肠杆菌、铜绿假单胞杆菌、金黄色葡萄球菌、枯草芽孢杆菌及白色念球菌均表现出较好的抑菌效果，其抑菌圈
分别为(11.39 ± 1.02)、(10.50 ± 0.92)、(10.50 ± 0.61)、(7.90 ± 0.79)和(8.31 ± 0.52) mm；桑叶银纳米粒对人宫颈癌
细胞〔半数抑制浓度（IC 50 ）为 60.63 mg/L〕、人肝癌细胞（IC 50 为 26.98 mg/L）和人乳腺癌细胞（IC 50 为 18.65 mg/L）
有很好的抑制作用。
关键词：桑叶水提物；银纳米粒；绿色合成；抗菌活性；抗癌活性；医药原料
中图分类号：TB34；TQ465.91；R979.1 文献标识码：A 文章编号：1003-5214 (2021) 01-0130-08

Green synthesis of silver nanoparticles with aqueous Folium Mori
extracts and their antimicrobial and anticancer activities

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WANG Chunli, CHEN Zhongqin, XU Leilei, CHEN Haixia
（School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China）

Abstract: To better develop silver nanoparticles with good physicochemical properties and bioactivities.
Mulberry leaves silver nanoparticles (ML-AgNPs) were prepared from aqueous mulberry leaves extracts
(MLE) by green synthesis method. The factors affecting the formation of ML-AgNPs such as concentration
of AgNO 3 solution, reaction temperature, volume ratio of MLE solution to AgNO 3 solution, pH of MLE
solution and reaction time were investigated. The products were characterized by UV-Vis, SEM and FTIR.
The antimicrobial activities of ML-AgNPs were assessed by determining the inhibition zone and minimum
inhibitory concentration (MIC) values, and their anticancer activities were evaluated by cell cytotoxicity
assay. The results showed that the optimal conditions for the synthesis of ML-AgNPs were as follows:
AgNO 3 concentration 5.0 mmol/L, V(MLE)∶V(AgNO 3 solution)=1∶5, reaction temperature 35 ℃, pH of
MLE solution 11.0, and reaction time 6 h. Under the above-mentioned conditions, the synthesized
ML-AgNPs were spherical with an average particle size (48.78 ± 0.39) nm and low surface potential (–27.8 ±
2.00) mV. Compared with MLE, ML-AgNPs showed excellent antimicrobial activities against E. coli, P.
aeruginosa, S. aureus, B. subtilis and C. albicans with inhibition zone values of (11.39 ± 1.02), (10.50 ±
0.92), (10.50 ± 0.61), (7.90 ± 0.79) and (8.31 ± 0.52) mm, respectively. Moreover, ML-AgNPs exhibited
good anticancer activities against Hela, HepG2 and MCF-7 cell lines with median inhibition concentration
IC 50 of 60.63, 26.98 and 18.65 mg/L, respectively.
Key words: aqueous Folium Mori extracts; silver nanoparticles; green synthesis; antimicrobial activity;
anticancer activity; drug nlaterials

收稿日期：2020-06-20; 定用日期：2020-09-03; DOI: 10.13550/j.jxhg.20200540
基金项目：天津市科技基金（18PTZWHZ00190）
作者简介：王春莉（1992—），女，硕士生。联系人：陈海霞（1974—），女，教授，E-mail：chenhx@tju.edu.cn。