Page 161 - 《精细化工》2021年第4期

第 38 卷第 4 期 精 细 化 工 Vol.38, No.4
202 1 年 4 月 FINE CHEMICALS Apr. 2021

医药与日化原料
汉麻叶中大麻二酚的分离纯化及其抗抑郁功能

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王语聪 ，谢智鑫 ，杨文敏 ，张宏伟 ，刘 江 ，韩建春 1,2*
（1. 东北农业大学 食品学院，哈尔滨 黑龙江 150030；2. 黑龙江省绿色食品科学研究院，哈尔滨 黑龙
江 150028；3. 黑龙江省哈麻生物科技有限公司，哈尔滨 黑龙江 150030）
摘要：采用柱层析技术对汉麻叶中大麻二酚（CBD）进行分离纯化，并探究其在抑郁症状方面的应用。汉麻叶
经预处理后通过超临界 CO 2 萃取得到汉麻浸膏，然后采用树脂两步色谱方法，得到高纯度 CBD，并建立慢性不
可预测的轻度应激模型小鼠，采用灌胃 CBD 方式来探究其抑郁行为及对炎症因子的影响。结果表明，通过该法
分离纯化提取 CBD 的纯度高达 99.19%；通过动物实验得出，与模型组相比，阳性对照组及 CBD 高低剂量组均
显著改善模型小鼠的抑郁行为（P<0.05）；模型组小鼠的皮层和海马组织中肿瘤坏死因子（TNF-α）、白细胞介素-1
（IL-1β）和一氧化氮合酶（iNos）mRNA 表达量显著升高（P<0.05）；CBD 剂量组小鼠的皮层和海马组织中 TNF-α、
IL-1β 和 iNos mRNA 表达量均显著降低（P<0.05），说明 CBD 能够有效改善小鼠的抑郁行为和抑制脑组织皮层
和海马组织的炎症反应。
关键词：汉麻叶；大麻二酚；抑郁；炎症；分离；纯化；医药原料
中图分类号：R285.5 文献标识码：A 文章编号：1003-5214 (2021) 04-0795-07

Separation, purification and antidepressant function of
cannabidiol from Cannabis sativa leaves

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WANG Yucong , XIE Zhixin , YANG Wenmin , ZHANG Hongwei ,
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LIU Jiang , HAN Jianchun 1,2*
（1. College of Food Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China; 2. Heilongjiang
Green Food Science Research Institute, Harbin 150028, Heilongjiang, China; 3. Heilongjiang Hama Biological
Technology Co., Ltd., Harbin 150030, Heilongjiang, China）
Abstract: Cannabidiol (CBD) was isolated and purified from Cannabis sativa leaves by column
chromatography. And its application in depressive symptoms was explored. The Cannabis sativa extract
was obtained by supercritical CO 2 extraction of pretreated Cannabis sativa leaves. Then, cannabidio with
high purity was achieved by a two-step chromatography process. Chronic unpredictable mild stress model
mice were established. Intragastric administration of CBD was used to investigate the depressive behavior
and the effect on inflammatory factors. The results showed that the purity of CBD extracted by the
above-mentioned method was 99.19%. The depressive behavior of model mice was significantly improved
by CBD dose (P<0.05), and the expression levels of tumor necrosis factor (TNF-α), interleukins-1 (IL-1β)
and nitric oxide synthase (iNos) mRNA in cortex and hippocampus of model mice were all significantly
increased (P<0.05), while the expression levels of TNF-α, IL-1β and iNos mRNA in the cortex and
hippocampus of mice in the CBD dose group were all significantly reduced (P<0.05), indicating that CBD
could effectively improve the depressive behavior of mice and inhibit the inflammatory response in the
cortex and hippocampus of brain tissue.
Key words: Cannabis sativa leaf; cannabidiol; depression; inflammation; isolation; purification; pharmaceutical
materials

收稿日期：2020-09-08; 定用日期：2020-11-04; DOI: 10.13550/j.jxhg.20200836
基金项目：黑龙江省绿色食品科学研究院课题（SKY20200715-1）
作者简介：王语聪（1994—），女，硕士生，E-mail：wangyucong1115@163.com。联系人：韩建春（1973—），男，教授，E-mail：hanjianchun@hotmail.com。