Page 107 - 《精细化工》2021年第6期
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第 6 期 刘 春,等: 菌团 HBB 降解竹纤维素纤维及微生物群落功能分析 ·1169·
物组成;厚壁菌门、变形菌门、以及一定数量的拟
杆菌门、放线菌门、疣微菌门是主要降解期的优势
菌门 ;而 促 使 BCF 高效 降解 的主要 菌属 包 括
Brevibacillus、Devosia、Ochrobactrum、Taibaiella、
Pseudoxanthomona、Azospirillum、Cellulomonas。
微生物菌团 HBB 为竹纤维的高效降解提供了
新思路。降解过程中代谢产物(如可发酵性糖)的
产生和积累仍需进一步探究。
参考文献:
[1] ZHAO X B, ZHANG L H, LIU D H, et al. Biomass recalcitrance.
PartⅠ: The chemical compositions and physical structures affecting
the enzymatic hydrolysis of lignocelluloses[J]. Biofuels, Bioproducts
and Biorefining, 2012, 6(4): 465-482.
[2] WANG P (王平), SONG Z H (宋振华), CHANG J (常娟), et al.
Research progress on corp straw as livestock feed[J]. Feed Research
(饲料研究), 2019, 42(3): 87-90.
[3] XU J (徐杰), XU X H (许修宏). Effect of streptomyces griseorubens
C-5 on lignocellulose degradation and microbial community metabolic
of compost[J]. Acta Energiae Solaris Sinica (太阳能学报), 2018, 39(2):
285-291.
[4] FAN C (范超). Efficient ethanol production from lignocellulosic
hydrolysates by strain adaptation and repeated fermentation[D].
Shanghai: Shanghai Jiao Tong University (上海交通大学), 2013.
[5] LI X (李鑫), YONG Q (勇强). Production of fumaric acid from
lignocellulose by Rhizopus oryzae[J]. Chemistry and Industry of
Forest Products (林产化学与化工), 2020, 40(1): 1-7.
[6] CARRÈRE H, DUMAS C, BATTIMELLI A, et al. Pretreatment
methods to improve sludge anaerobic degradability: A review[J].
Journal of Hazardous Materials, 2010, 183(1): 1-15.
[7] HENDRIKS A, ZEEMAN G. Pretreatments to enhance the
digestibility of lignocellulosic biomass[J]. Bioresource Technology,
a=门水平 b=属水平 2009, 100(1): 10-18.
图 6 不同时期 HBB 细菌组成及进化 [8] LIANG J J, PENG X, YIN D X, et al. Screening of a microbial
consortium for highly simultaneous degradation of lignocellulose and
Fig. 6 Composition and evolution of HBB bacteria at
different stages chlorophenols[J]. Bioresource Technology, 2015, 190: 381-387.
[9] KOCHER G S, KAUR P, TAGGAR M S. An overview of pretreatment
processes with special reference to biological pretreatment for rice
3 结论 straw delignification[J]. Current Biochemical Engineering, 2017, 4(3):
151-163.
[10] PANDA A K, BISHT S S, DEMONDAL S, et al. Brevibacillus as a
(1)3 种微生物策略的降解可分为主要降解期 biological tool: A short review[J]. Antonie van Leeuwenhoek, 2014,
(0~11 d)和降解后期(12~15 d);主解期,HBB 105(4): 623-639.
组具有最高的 CMCase 酶活性、漆酶活性和丰富的 [11] KAMSANI N, SALLEN M M, YAHYA A, et al. Production of
lignocellulolytic lnzymes by microorganisms isolated from Bulbitermes
木聚糖酶活性。此期间,HBB 菌群生长良好,pH= sp. termite gut in solid-state fermentation[J]. Waste and Biomass
6.80~7.84 是 HBB 发酵产酶的较适宜环境。发酵结束 Valorization, 2016, 7(2): 357-371.
[12] YU H J (于慧娟), GUO X L (郭夏丽). Screening of straw-degrading
时,HBB 的降解效果最佳,干物质损失率为 77.67%。 strains and their enzyme-producing conditions[J]. Biotechnology
(2)根据黏度法、FTIR 和 XRD 的检测结果, Bulletin (生物技术通报), 2019, 35(2): 58-63.
底物组分结构在早期即被破坏(0~7 d),结构特征 [13] XU G H (许光辉), ZHENG H Y (郑洪元). Manual of methods for
soil microbial analysis[M]. Beijing: Agriculture Press (农业出版社),
的改变是促进酶水解作用有效发挥和 BCF 加速降解 1986.
的重要原因。 [14] WANG S (王爽), LI Y (李赢), LYU C (吕超). Effects of nitrogen
fertilizer and density on protein accumulation and content of barley
(3)根据高通量测序结果,降解过程中获得的
grain (Hordeum vulgare L.)[J]. Molecular Plant Breeding (分子植物
HBB 样本的细菌群落涵盖 18 门,26 纲,55 目,85 育种), 2019, 17(11): 3788-3793.
科,143 属;PCoA 和 Anosim 分析结果进一步表明, [15] XU J (徐杰). Isolation and identification of rice straw biodegrading
actinomycetes and its degradation mechanism[D]. Harbin: Harbin
HBB0、HBB7 和 HBB15 的细菌群落结构差异显著, Institute of Technology (哈尔滨工业大学), 2011.
说明降解过程中不同时期存在各自典型的功能微生 [16] BOURBONNAIS R, PAICE M G. Oxidation of non-phenolic substrates