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第 7 期 郭林新,等: 响应面优化马桑皮没食子酸的酶法提取技术 ·1429·
NKA-9 大孔吸附树脂,以质量分数 30%乙醇水溶液 Occidentalis testa for producing the industrially important gallic acid
为洗脱剂进行纯化,并结合重结晶的方法,得到纯 through submerged fermentation[J]. World Journal of Pharmaceutical
Research, 2016, 5(8): 861-864.
13
1
化没食子酸。通过 HNMR、 CNMR 及二维 HSQC [11] SHARMA K P, JOHN P J, GOSWAMI P, et al. Enzymatic synthesis
对没食子酸的结构进行了鉴定。采用酶法制备的没 of gallic acid from tannic acid with an inducible hydrolase of
Enterobacter spp.[J]. Biocatal Biotransform, 2017, 35(3): 177-184.
食子酸的纯度为 96.0%,明显高于传统酸提取法制
[12] CHEN P X (陈培旭), LIU C L (刘春利), WANG S Y (王思渊), et al.
备的没食子酸纯度 81.5%。酶法制备的没食子酸具 Optimization of gallic acid production by enzymatic hydrolysis of
有更显著的抗氧化活性,对 DPPH•和•OH 具有明显 tara tannin[J]. Modern Food Science and Technology (现代食品科
技), 2020, 36(12): 60-68.
3+
的清除作用,并具有较强的 Fe 还原能力。本研究 [13] YANG Q M (杨秋明), RU Y (茹毅), WENG H F (翁惠芬), et al.
所建立的工艺方法可有效地从马桑皮中提取得到没 Simultaneous extraction and enzymatic hydrolysis of gallnut tannic acid[J].
Food Science and Technology (食品科技), 2020, 45(7): 235-241.
食子酸,这为没食子酸的天然产物来源提供了途径。
[14] BARGALI K, MANRAL V, BARGALI S S. Weight loss patterns in
decomposing litter of Coriaria nepalensis, an actinorhizal shrub from
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