Page 148 - 《精细化工》2021年第7期

第 38 卷第 7 期 精 细 化 工 Vol.38, No.7
202 1 年 7 月 FINE CHEMICALS July 2021

医药与日化原料
血小板膜伪装介孔聚多巴胺纳米药物

传递系统的制备及应用

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任丹丹，吴 梦，肖天钰，朱利民
（东华大学 化学化工与生物工程学院，上海 201620）

摘要：以三甲基苯为模板剂，采用一锅法制备了具有介孔结构的聚多巴胺纳米粒子（MPDA）。通过静电吸附负
载盐酸阿霉素（DOX），通过血小板膜（PLTM）仿生伪装得到 PLTM-DOX@MPDA 纳米粒子。采用 TEM、纳
米粒度分析仪、BET 和 UV-Vis 对纳米粒子的性质、形貌和粒径进行表征。结果表明，MPDA 表面具有清晰的
介孔结构，经 PLTM 包裹后的 PLTM-DOX@MPDA 平均粒径约为 184 nm。MPDA 孔径主要分布于 45 nm 左右，
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孔容为 0.6232 cm /g，比表面积高达 61.181 m /g，该介孔结构支持 MPDA 作为高效的药物传递系统。体外释药、
体外细胞摄取和体外细胞毒性实验结果表明，PLTM-DOX@MPDA 具有 pH 响应性控制药物释放，可以实现药
物缓释，可以避免巨噬细胞吞噬并且主动靶向癌细胞，可显著提高 DOX 对人乳腺癌细胞（MDA-MB-231）细
胞的杀伤作用。
关键词：介孔结构；纳米药物传递系统；聚多巴胺；药物释放；血小板膜；医药原料
中图分类号：TB383.1；TQ460.4 文献标识码：A 文章编号：1003-5214 (2021) 07-1430-07

Preparation and application of mesoporous polydopamine nano-drug
delivery system camouflaged by platelet membrane

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REN Dandan, WU Meng, XIAO Tianyu, ZHU Limin
（College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China）

Abstract: Polydopamine nanoparticles with mesoporous structure (MPDA) were prepared by one-pot
method using 1,3,5-trimethylbenzene as template. Doxorubicin hydrochloride (DOX) was loaded by
electrostatic adsorption and the nanodrug delivery system PLTM-DOX@MPDA was obtained by
biomimetic camouflage of platelet membrane (PLTM). The properties, morphology and particle size of the
nanoparticles were characterized by TEM, nanoparticle size analyzer, BET and UV-Vis spectrophotometer.
The results showed that the surface of MPDA had a clear mesoporous structure, and the average particle
size of PLTM-DOX@MPDA nanoparticles coated by platelet membrane was about 184 nm. MPDA had a
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pore size of about 45 nm, pore volume of 0.6232 cm /g and specific surface area of as high as 61.181 m /g.
This mesoporous structure supported MPDA as a highly efficient drug delivery system. In vitro drug release,
in vitro cell uptake and in vitro cytotoxicity experiments indicated that PLTM-DOX@MPDA had a
pH-responsive controlled drug release, which could achieve sustained drug release, avoid phagocytosis of
macrophages and actively target cancer cells, and significantly enhance the killing effect of DOX on human
breast cancer cells (MDA-MB-231).
Key words: mesoporous structure; nano drug delivery system; polydopamine; drug release; platelet membrane;
drug materials

癌症已被公认为人类健康的主要威胁之一。化 学疗法作为治疗癌症的主要方法仍面临巨大的挑

收稿日期：2021-01-04; 定用日期：2021-03-09; DOI: 10.13550/j.jxhg.20210008
基金项目：上海市科学技术委员会国际资助项目（16410723700）；生物医用纺织材料“111 工程”中国教育部（B07024）
作者简介：任丹丹（1997—），女，硕士生，E-mail：2574125897@qq.com。联系人：朱利民（1960—），男，教授，E-mail：lzhu@dhu.edu.cn。