Page 65 - 《精细化工》2021年第8期

第 38 卷第 8 期 精 细 化 工 Vol.38, No.8
202 1 年 8 月 FINE CHEMICALS Aug. 2021

功能材料
pH 响应虾青素羧甲基壳聚糖微球的制备与应用

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窦 翔 ，王政武 ，贺高红 1,2 ，张文君 ，张秀娟 1,*
（1. 大连理工大学 化工学院盘锦分院 精细化工国家重点实验室，辽宁 盘锦 124221；2. 大连理工大学
化工学院 膜科学与技术研发中心，辽宁 大连 116024）

摘要：以氯乙酸和壳聚糖为原料，通过 N,O-羧甲基化化学改性方法制备了水溶性羧甲基壳聚糖，并以此为原料
通过乳液法制备羧甲基壳聚糖微球和羧甲基壳聚糖负载虾青素微球。考察了羧甲基壳聚糖微球的形态、分散性
和粒径。结果表明，微球最佳制备条件为：羧甲基壳聚糖含量（以水相即去离子水质量为基准，下同）1.0%，
油水体积比 1∶1，表面活性剂 Span 80 含量（以油相即液体石蜡体积为基准，下同）4.0%，交联剂戊二醛含量
（以油相即液体石蜡体积为基准，下同）7.5%，分散转速 4000 r/min。红外测试表明，微球成功负载虾青素，
虾青素初始添加量为 20 mg 时，微球的载药率和包封率分别为 10.74%±0.1%和 67.24%±0.2%。在模拟胃液和模
拟肠液中，负载虾青素微球的释放率分别约为 10%和 85%。羧甲基壳聚糖可以用作药物载体。
关键词：虾青素；羧甲基壳聚糖；乳化法；控释；载药微球；功能材料
中图分类号：TQ469；TQ450.4+2 文献标识码：A 文章编号：1003-5214 (2021) 08-1559-05

Preparation and application of pH-responsive loaded with

carboxymethyl chitosan microspheres

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DOU Xiang , WANG Zhengwu , HE Gaohong , ZHANG Wenjun , ZHANG Xiujuan
（1. State Key Laboratory of Fine Chemicals, Panjin Branch, School of Chemical Engineering, Dalian University of
Technology, Panjin 124221, Liaoning, China; 2. Research and Development Center of Membrane Science and
Technology, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, Liaoning, China）
Abstract: Water-soluble carboxymethyl chitosan was prepared by chemical modification of N,O-
carboxymethyl chitosan with chloroacetic acid and chitosan as raw materials. Carboxymethyl chitosan
microspheres and carboxymethyl chitosan microspheres loaded with astaxanthin were prepared by emulsion
method from water-soluble carboxymethyl chitosan. The morphology, dispersion and particle size of
carboxymethyl chitosan microspheres were investigated. The results showed that the optimal preparation
conditions of microspheres were as follows: content of carboxymethyl chitosan [based on water phase
(deionized water) mass, the same below] of 1.0%, volume ratio of oil to water of 1∶1, content of Span 80
[based on oil phase (liquid paraffin) volume, the same below] of 4.0%, content of glutaraldehyde [based on
oil phase (liquid paraffin) volume, the same below] of 7.5%, and dispersion speed of 4000 r/min. FTIR
results indicated that the microspheres were successfully loaded with astaxanthin, and the loading rate and
encapsulation rate of the prepared microspheres with a initial astaxanthin dosage of 20 mg were
10.74%±0.1% and 67.24%±0.2%, respectively. The release rates of astaxanthin loaded microspheres in
simulated gastric juice and simulated intestinal juice were about 10% and 85%, respectively.
Carboxymethyl chitosan could be used as a drug carrier.
Key words: astaxanthin; carboxymethyl chitosan; emulsification method; controlled release; drug-load
microspheres; functional materials

收稿日期：2021-02-23; 定用日期：2021-04-22; DOI: 10.13550/j.jxhg.20210188
基金项目：国家自然科学基金（31400840）
作者简介：窦 翔（1994—），男，硕士生，E-mail：1832393573@qq.com。联系人：张秀娟（1978—），女，副教授，E-mail：xiujuanzhang@
dlut.edu.cn。