Page 124 - 《精细化工》2022年第12期

·2490· 精细化工 FINE CHEMICALS 第 39 卷

线粒体膜电位增加细胞内活性氧簇的产生，导致细 品科学), 2018, 39(21): 330-339.
[7] XIE B (谢博), FU H (傅红), YANG F (杨方). Research progress on
胞的氧化损伤死亡；除此之外，小分子肽也可以与
preparation purification, identification and structure-activity relationship
细胞内的一些蛋白质和酶结合，进而影响它们的功 of bioactive peptides[J]. Science and Technology of Food Industry
能和活性，甚至可以破坏 DNA 双链结构或者直接结 (食品工业科技), 2021, 42(5): 383-391.
合 DNA 来干扰其复制 [41] 。因此，具有小相对分子 [8] ZONG J (宗静). Studies on extraction and separation and some
biological activities of peptides from fruiting bodies of Cordyceps
质量的蚕蛹虫草多肽可以进入癌细胞，影响癌细胞 militaris[D]. Chongqing: Southwest University (西南大学), 2012.
正常的结构和功能，最终导致癌细胞死亡。 [9] QIAN W W (钱卫文), QIAN K N (钱康南), ZHANG T B (张天宝).
The preparation methods and applications of Cordyceps polypeptide:
3 结论 CN1314813C[P]. 2007-05-09.
[10] XU H Y (徐鸿雁), MENG S N (孟胜楠), ZHANG S Y (张疏雨),
et al. Study on the technology of protein degradation of Cordyceps
（1）通过单因素结合正交实验的方法对蚕蛹虫 militaris[J]. Journal of Chinese Institute of Food Science and Technology
草蛋白质的提取工艺条件进行优化，当提取条件为： (中国食品学报), 2014, 14(4): 127-135.
[11] JACK H, WONG J H, NG T B, et al. Cordymin, an antifungal
pH 8.5、料液比 1∶28、提取时间 3.5 h、提取 3 次；
peptide from the medicinal fungus Cordyceps militaris[J]. Phytomedicine,
蛋白质含量最高，可达到 45.06%。 2011, 18: 387-392.
（2）通过单因素结合正交实验的方法对蚕蛹虫 [12] JIANG H P (蒋海萍), LIAO D K (廖丹葵), TONG Z F (童张法).
Research progress of antioxidative peptides[J]. Guangxi Sciences (广
草多肽的制备工艺条件进行优化，最佳工艺为：碱
西科学), 2015, 22(1): 60-64.
性蛋白酶与木瓜蛋白酶酶活力比 4∶3、酶解最佳 [13] GUO S (郭尚), XU L N (徐莉娜), LI Y T (李艳婷), et al. Screening
pH 7.2、温度 55 ℃、加酶量 7000 U/mL、酶解时间 of excellent strains of Cordyceps militaris[J]. Journal of Shanxi
Agricultural University (Natural Science Edition) (山西农业大学学
3.5 h，多肽含量为 16.73%。
报: 自然科学版), 2020, 40(1): 17-21.
（3）在此酶解条件下制备所得蚕蛹虫草多肽（相 [14] GUO S (郭尚), XU L N (徐莉娜), LI Y T (李艳婷), et al. High
3
对分子质量<3.0×10 Da）对大肠杆菌、枯草芽孢杆 efficient cultivation techniques of silkworm Cordyceps militaris[J].
Edible Fungi (食用菌), 2019, 41(3): 46-48.
菌及金黄色葡萄球菌均表现出较好的抑菌效果；同 [15] ZHANG Y, LI Y J, HUANG J Y, et al. Comparison of characteristics
时，对骨肉瘤 Sao-S 细胞和膀胱癌 T24 细胞有很好 and contents of Cordyceps militaris cultivated with pupae of different
tussah silkworm varieties[J]. North Sericulture (北方蚕业), 2022,
的增殖抑制作用。
43(2): 7-11.
（4）在本文所建立的复合酶解条件下，所制备 [16] ZHANG N (张囡). Study on preparation, separation and biological
的蚕蛹虫草多肽具有良好的生物活性和较强的抗菌 activity of Lentinus Edodes stalk polypeptide[D]. Xi'an: Northwest
University (西北大学), 2019.
抗癌活性，具有潜在的医药应用价值。 [17] XU L N, GUO S, ZHANG S W. Effects of solid-state fermentation
on the nutritional components and antioxidant properties from quinoa[J].
参考文献： Emirates Journal of Food and Agriculture, 2019, 31(1): 39-45.
[1] NXUMALO W, ELATEEQ A A, SUN Y F. Can Cordyceps cicadae [18] XU L N, GUO S, ZHANG S W. Effects of solid-state fermentation
be used as an alternative to Cordyceps militaris and Cordyceps with three higher fungi on the total phenol contents and antioxidant
sinensis?–A review[J]. Journal of Ethnopharmacology, 2020, 257: 112879. properties of diverse cereal grains[J]. Fems Microbiology Letter, 2018,
[2] YANG X Q, LIN P, WANG J, et al. Purification, characterization and 365(16): 132-140.
anti-atherosclerotic effects of the polysaccharides from the fruiting [19] WU Q (吴淇), ZOU L H (邹亮华), HU X Y (胡学一), et al. Synthesis
body of Cordyceps militaris[J]. International Journal of Biological and antioxidant and bacteriostatic properties of cannabidiol analogs[J].
Macromolecules, 2021, 181: 890-904. Fine Chemicals (精细化工), 2022, 39(3): 584-591.
[3] SINGPOONGA N, RITTIRON R, SEANG-ON B, et al. Determination [20] GAO L (高莉), LIU L L (刘琳琳), WANG R Q (王瑞琪), et al.
of adenosine and cordycepin concentrations in Cordyceps militaris Preparation and property of amino acid modified melanin derived
fruiting bodies using near-infrared spectroscopy[J]. ACS Omega, from apricot kernel shells[J]. Fine Chemicals (精细化工), 2022,
2020, 5(42): 27235-27244. 39(7): 1393-1401.
[4] LIU Y T (刘元涛), WANG J R (王建瑞), LI H (李虹), et al. Effects [21] CHENG S (程爽), HE F (贺斐), FU L Y (付龙洋), et al. Preparation
of enriching chromium ion on biomass and chemical composition of of selenium polysaccharide from Rabdosia rubescens and analysis of
fruit body of Cordyceps militaris[J]. Mycosystema (菌物学报), its antioxidant activity[J]. Fine Chemicals (精细化工), 2021, 38(10):
2022, 3(25): 1-11. 2064-2071.
[5] GUO J K (郭静科), YU X W (俞晓卫), LIU S T (刘树滔), et al. [22] LIANG Y Q (梁永琴), YANG X H (杨喜花), ZHAO L L (赵莉莉),
Antioxidant properties of powders of Cordyceps militaris fungus, et al. Screening of antibreast cancer active componenents and effect
sporophore and mycelium and their effect on body surface along of Sappanl L.[J]. Journal of Shanxi University (National Science
acupuncture meridians[J]. Journal of Food Science and Technology Edition) (山西大学学报: 自然科学版), 2022, 45(2): 465-472.
(食品科学技术学报), 2022, 40(1): 93-99. [23] ZHANG J F (张建锋). Analysis of nutritional components and health
[6] ZUO J H (左锦辉), GONG X Y (贡晓燕), DONG Y M (董银卯), care function of edible fungi[J]. Edible Fungus of China (中国食用
et al. Research achievements in bioactive components, pharmacological 菌), 2020, 39(7): 185-187.
effects and applications of Cordyceps militaris[J]. Food Science (食 [24] LI S (李思), MENG M (孟萌), MA Y H (马彦华), et al. Optimization of
protein extraction from Morchella by response surface methodology[J].