Page 66 - 《精细化工》2022年第5期

·920· 精细化工 FINE CHEMICALS 第 39 卷

3,4-DCA 的解毒，而本实验证明细菌中同样存在 N- A study on the fate of the priority pollutant in a sediment-water
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and its degradability[J]. Fine Chemicals (精细化工), 2004, 21(12):
图 8 DTNB 测定乙酰辅酶 A 的水解 913-916.
Fig. 8 Determination of hydrolysis of acetyl-CoA [10] ZHANG J, SUN J Q, YUAN Q Y, et al. Characterization of the
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本研究从染料废水中筛选出能够转化 3,4-DCA Catalytic properties of resting cells from nitrilase producing
microorganism Pseudomonas putida XY4[J]. Fine Chemicals (精细
的菌株，并利用其中两株菌构建混合菌群，对混合
化工), 2014, 31(12): 1466-1470.
菌群转化条件进行了优化，并进一步通过代谢产物 [12] WANG S J, POON K, CAI Z W. Biodegradation and removal of
的分析探讨了转化机理，得出以下结论： 3,4-dichloroaniline by Chlorella pyrenoidosa based on liquid
chromatography-electrospray ionization-mass spectrometry[J]. Environ
（1）通过形态学观察及 16S rDNA 序列分析， Sci Pollut Res, 2013, 20: 552-557.
初步推断两株菌均为铜绿假单胞菌，混合菌群对 [13] TRAVKIN V V M, SOLYANIKOVA I I P, RIETJENS I I M C M, et al.
3,4-DCA 转化率明显高于单菌。 Degradation of 3,4-dichloro- and 3,4-difluoroaniline by Pseudomonas
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（2）混合菌群对 3,4-DCA 具有较强的转化代谢 Part B, 2003, 38(2): 121-132.
潜力，3 d 内对 100 mg/L 3,4-DCA 的转化率达到 [14] XIAO H X, KUCKELKORN J, NÜßER L K, et al. The metabolite
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than the parent compounds 3,4-dichloroaniline (3,4-DCA) and
（3）混合菌群主要通过乙酰化途径转化 3,4-DCA， propanil[J]. Science of the Total Environment, 2016, 551/552:
代谢产物为 3,4-DCAA。 304-316.
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与单一菌株相比，混合菌群对 3,4-DCA 的转化 3,4-dichloroaniline[D]. Chongqing: Southwest University (西南大
能力更强，但机理尚不明确，且乙酰化产物无法进 学), 2014.
一步降解，需后续实验进一步探索。 [16] LI T, DENG X P, WANG J J, et al. Biodegradation of
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