Page 123 - 《精细化工》2022年第7期

第 7 期 董其惠，等: 双相深共熔溶剂提取的银杏果外种皮活性成分的分离回收
附 6 h，解吸剂为无水乙醇（其中，NaOH 添加量为
0.05 mol/L），静态解吸 8 h。D201 树脂：疏水相 DES
提取液用无水乙醇稀释 5 倍，25 ℃吸附 6 h，解吸
剂为体积分数 75%的乙醇水溶液（其中，HCl 添加
量为 0.01 mol/L），静态解吸 2 h，在此条件下 D201
树脂对银杏酚酸的吸附率为 71.73%，1 次解吸率为
94.54%，银杏酚酸回收率为 67.81%。由于 HYA-502B
树脂的 1 次解吸率为 33.43%，因此，对 HYA-502B
树脂进行两次解吸实验，HYA-502B 树脂第 2 次解
吸率为 66.08%，两次总解吸率可达 99.51%，推测
是因为第 2 次静态解吸过程中树脂有一部分破损，有
利于树脂中银杏酚酸的释放，因此，第 2 次的解吸效
果要明显优于第 1 次的解吸效果。最终选择 HYA502
树脂为疏水相 DES 提取液中银杏酚酸回收的最佳树
脂，其对银杏酚酸的吸附率可达 93.79%，总解吸率
可达 99.51%，最终银杏酚酸回收率可达 93.33%。
3 结论
在本课题组前期研究基础上建立了回收银杏果
外种皮活性成分的方法。经过一系列的优化实验，
最终结果如下：
（1）对于亲水相 DES 提取液中的活性成分——
萜内酯、黄酮、原花青素，采用 EOPO 萃取回收的
方法。选用 EOPO 分子量 2650，质量分数为 90%的
EOPO 水溶液与亲水相 DES 提取液按质量比 4∶6
组成回收体系，混合离心后第 1 次重新形成双相，
此时活性成分富集在 EOPO 相中，分离出 EOPO 富
集相，在 70 ℃下水浴 90 min，离心后第 2 次重新
形成双相，此时活性成分富集在下相水溶液中，其
中萜内酯、黄酮和原花青素的回收率分别可达
89.44%、53.27%和 29.77%。
（2）对于疏水相 DES 提取液中活性成分——银
杏酚酸，采用大孔树脂吸附回收的方法。选用
HYA-502B 树脂进行静态吸附-解吸回收，疏水相
DES 提取液用无水乙醇稀释 3 倍后用树脂静态吸附
6 h，样液体积为 10 mL，树脂用量为 1 g。解吸剂
为碱添加量为 0.05 mol/L 的无水乙醇，解吸剂用量
为 10 mL。静态解吸 8 h，重复解吸 2 次，在此条件
下，大孔树脂对银杏酚酸吸附率为 93.79%，总解吸
率为 99.51%，银杏酚酸的回收率可达 93.33%。
本研究成功建立了分别从亲水相 DES 和疏水相
DES 中回收银杏果外种皮中萜内酯、黄酮、原花青
素和银杏酚酸的方法，该法具有高效、绿色、安全、
操作简便等优点，具有良好应用前景。
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