Page 133 - 《精细化工》2022年第8期

第 39 卷第 8 期 精 细 化 工 Vol.39, No.8
2022 年 8 月 FINE CHEMICALS Aug. 2022

中药现代化技术
超高压微射流技术提取对裸藻 β-葡聚糖

结构和抗氧化活性的影响

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乜世成，张 炜 ，高 红，宋 林，隋成博，荆永康
（青海师范大学 化学化工学院，青海 西宁 810008）

摘要：利用超高压微射流技术（UHPM）对裸藻 β-葡聚糖（EBG）进行提取，通过单因素实验及响应面优化设
计实验考察了时间、压力、料液比及十二烷基硫酸钠（SDS）质量浓度对 EBG 提取率的影响，同时采用刚果红
实验以及 FTIR、XRD、SEM 对其结构进行了表征，还比较了裸藻 β-葡聚糖标准品（EBGS）和 EBG 对 1,1-二
苯基-2-三硝基苯肼自由基（DPPH•）和•OH 的清除能力。结果表明，EBG 提取最佳条件为微射流时间 93 s、微
射流压力 34 MPa、料液比（g∶mL，下同）1∶270、SDS 质量浓度 4.2 g/L。在此条件下，EBG 提取率为 80.27%，
与理论值 81.69%接近。EBG 具有有序的三螺旋结构，UHPM 使 EBG 的结晶度减小，EBG 表面出现裂痕与空隙。
在质量浓度为 0.4~2.0 g/L 时，EBGS 和 EBG 对 DPPH•清除率基本相同，在质量浓度为 4.0 g/L 时，EBG 对 DPPH•
清除率达到 50.26%，高于 EBGS 对 DPPH•的清除率（35.14%）；而 EBGS 和 EBG 对•OH 清除效果不明显，清
除率相对较低。
关键词：超高压微射流技术；裸藻 β-葡聚糖；响应面优化法；结构；抗氧化活性；中药现代化技术
中图分类号：O636.1 文献标识码：A 文章编号：1003-5214 (2022) 08-1633-09

Effect of ultra-high pressure micro-jet technology extraction on structure
and antioxidant activity of β-glucan from Euglena gracilis

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NIE Shicheng, ZHANG Wei , GAO Hong, SONG Lin, SUI Chengbo, JING Yongkang
(School of Chemistry and Chemical Engineering, Qinghai Normal University, Xining 810008, Qinghai, China)

Abstract: β-Glucan from Euglena gracilis (EBG) was extracted by ultra-high pressure micro-jet technology
(UHPM). The effects of time, pressure, solid-liquid ratio and sodium dodecyl sulfate (SDS) mass
concentration on the extraction rate were investigated by single factor experiments as well as response
surface optimization design experiments. The structure of EBG was characterized and analyzed by congo
red experiment, FTIR, XRD and SEM. Furthermore, experiments were conducted to explore the scavenging
abilities of EBG and its standard (EBGS) towards 1, 1-diphenyl-2-trinitrophenylhydrazine free radicals
(DPPH•) and hydroxyl radical (•OH). The results showed that the optimum extraction conditions of EBG
were as follows: time of 93 s, pressure of 34 MPa, solid-liquid ratio (g∶mL) of 1∶270 and SDS mass
concentration of 4.2 g/L. Under these conditions, the extraction rate of EBG reached 80.27%, which was
close to the theoretical value of 81.69%. Characterization analyses indicated that EBG had an orderly triple
helix structure, but with surface cracks and voids due to the reduction of crystallinity induced by UHPM.
EBG demonstrated higher scavenging efficiency (50.26%) towards DPPH• in comparison to EBGS
(35.14%) at mass concentration of 4.0 g/L while showed similar scavenging rate at mass concentrations
from 0.4 g/L to 2.0 g/L. Meanwhile, it seemed that EBGS and EBG had no obvious scavenging effect on
•OH with relatively low scavenging rates.
Key words: ultra-high pressure micro-jet; β-glucan from Euglena gracilis; response surface optimization
method; structure; antioxidant activity; modernization technology of traditional Chinese medicines

收稿日期：2021-11-21; 定用日期：2022-03-07; DOI: 10.13550/j.jxhg.20211286
基金项目：高原人工湿地生态平衡构建与应用示范（2021-SF-139）
作者简介：乜世成（1995—），男，硕士生，E-mail：nie950715@163.com。联系人：张 炜（1972—），女，教授，E-mail：zhangwei@
qhnu.edu.cn。