Page 101 - 《精细化工》2023年第4期
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す 4 ䷉⢶ྤ喑ぶ: ᵥ䚥ᣏ䦵ౕោ⩌㉍₸⪆⩌➖эᙌᷭ≸͚⮱Ꮑ⩕ g787g
ᵥ䚥ᣏ䦵ڤᰶᭀλԛ亝̻ߌ㘪ࡃ⮱➦◦喑ౕ ౕ℁㞟эᙌகᲱᐧ͚Ꮑ⩕Ꭼ∈喑ᣕߕγധλᵥ䚥ᣏ
Ჱᐧᴴ䃝ಸោ⩌㉍₸⪆㢔ٶэᙌக͚ڤᰶ̭Ⴧ⮱х 䦵⮱ᷭ≸ោ⩌㉍₸⪆℁㞟эᙌக⮱ࣾᆂȡLI ぶ [52]
߬ȡѳ㢔ٶᴴ䃝ಸᵥ䚥ᣏ䦵䕇፥ႅౕ᱙倅Ƞव ݖ⩕ࢎ䗐䰶㉍ᵥ䚥䔯䙺Ҁ̻ڣο㶒Ꮌ݄Ჱᐧࣹ䨫
䓴⼸ฺᱯȠ▢᩼Ꮣѻぶ㑧䮤喑ₑ喑Ჱᐧ䲋ᴴ䃝ಸ DNA ᣏ䦵喑ᑂڒᵥ䚥ใܴ䚣ē䭺㼐ࢂ䨫 DNA ⮱ह
㢔ٶэᙌக᭜̭䒰ສ⮱䔶᠖喑ຯᑂڒڤᰶ⾮ܧ㢔 ᬣϔ⩌䲣ᴴᓗ⣜Ԏतᩫ๔喑ᰭ㏵ݖ⩕䛾㏠ㆠ䷄ㆿহ
ٶ➦ᕔহԎतᩫ๔ߌ㘪⮱㏠ㆠᱽ̻᫆ᵥ䚥ᣏ䦵ࡼह ࢂ䨫 DNA ᄦ冩㯸⮪⮱》ζᕔ㐀वᄦ䲣ᴴࢎ䗐䰶
҉⩕喑䔈̭ₒ倅ោ⩌㉍㢔ٶ⩌➖эᙌக⮱ᷭ≸ᕔ ㉍ϔ⩌䷉㞟৺Ꮑ喍ఫ 5B喎ȡᲱᐧ⮱℁㞟эᙌகᄦ
㘪ȡౕ䓴ࣨ⮱܍Ꭱ䛹喑ऱڤ㢔ٶ➦ᕔ⮱ฺवᱽ᫆ ࢎ䗐䰶㉍㶕⣝ܧ䒰ც⮱㏬ᕔᷭ≸㠰డ喑हᬣ㶕⣝ܧ䒰
–14
ᵥ䚥ᣏ䦵̺⊹⣝喑ѳڣ㢔ٶᕔ㘪ᰶᒲ䔈̭ₒ倅喑 ܧ㞟⮱䔶᠖ᕔহ▢᩼ᕔ喑ᷭ≸䭽ѻ㜠 2.8×10 mol/Lȡ
᱗Გ䰭ᐭࣾ倅ٶ⽠ჇᕔȠ倅ٶ㜡ࣾٶ᩵⢴Ƞज䄰ᣔ⮱ RAMEZANI ぶ [53] 䃫䃎 DNA ̶㳧ܳၽᐭڠͧ䃳ݘ
㢔ٶ㏠ㆠᱽ᫆Ꭳⵁ⾣ڣࣾٶᱧݣ喑ͧᐧ⿸ധλᵥ䚥 ᣏ䦵喑В䛾㏠ㆠ䷄ㆿͧ⩌㞟ٰУ喑Ჱᐧγ̭▢᩼Ƞ
ᣏ䦵⮱ᷭ≸ោ⩌㉍₸⪆㢔ٶ⩌➖эᙌகӈᕊ䌜ȡ ᔘ䕌⮱℁㞟эᙌக喑⩕λᷭ≸ఈ⣜㉍喑ᎣߌᏁ⩕
λ➈ຣᵤ৮⮱ᷭ≸ȡ
3 ധλᵥ䚥ᣏ䦵⮱ᷭ≸ោ⩌㉍₸⪆℁㞟⩌
➖эᙌக
℁㞟⩌➖эᙌக᭜ᠴВ⩌㞟䄂ݯ⮱᭫㞟ࣺᏁͧ
ധ喑ᄳ㈨㐌͚⮱➖ࡃᕔ䉕䒙ᢏज㻳ࡃ䷉㞟⮱अ
ࡃ喑Ꭳ䕇䓴℁䒰ᝃ㔲≸䛼䷉㞟अࡃ⮱⼸ᏓᲒჇⰛ
ᴴ➖⮱䛼⮱̭⩌➖эᙌகȡ℁㞟эᙌகࣾ⩌⮱
䷉㞟अࡃ⩕㖶Ⱪࢠजݑ [44] 喑ڤᰶキࢂȠᭀ᧺҉Ƞ
ܳᲽ䕌ᏓᔘȠ᱙ѻぶх߬ [45-47] ȡധλᵥ䚥ᣏ䦵⮱
➦ᐯᕔ䃳ݘȠԎत䒙ᢏ̻ᩫ๔ߌ㘪喑जᲱᐧ℁㞟⩌
➖эᙌக⩕λោ⩌㉍₸⪆⮱ᷭ≸ܳᲽȡLUAN ぶ [48]
Ჱᐧγ̭ m-L-DNA ᣏ䦵喑ₑᣏ䦵⩞ԛ亝λⷮ㏠
ㆠ䷄ㆿ̷⮱ࣾᣏ䦵 H1 হԛ亝λ䛾ᆋᰶᱧᵳ᳣-䧯
㏠ㆠ䷄ㆿฺवᱽ᫆喍NMOF-Pt喎̷⮱Ԏत DNA ᣏ
䦵ᱯϑ㔹喍ఫ 5A喑ڣ͚喑NMOF ͧ⅕ധߌ㘪ࡃ䛾
ᆋᰶᱧᵳ᳣喠TMB ͧ 3,3,5,5-ఈ⩟ധ㖁㠜㘧喎ȡⰛᴴ
➖ࢎ䗐䰶㉍㼓ࣾ m-L-DNA ᣏ䦵̻䒲ߖᣏ䦵 H2 ϔ⩌
יࡃࣾ㏱㷲䒲ߖ⮱䲣ᴴᓗ⣜Ԏतᩫ๔喑倅γэ
ఫ 5 ധλ m-L-DNA ᣏ䦵⮱ࢎ䗐䰶㉍℁㞟⩌➖эᙌக
ᙌக⮱▢᩼Ꮣ喑ᷭ≸䭽ͧ 0.2 pg/mLȡₑใ喑䄒эᙌ ᘼఫ喍A喎 [48] 喠ധλ DNA ᣏ䦵֣㖁䛾㏠ㆠ䷄ㆿ⮱
கᄦࢎ䗐䰶㉍㶕⣝ܧᲮ倅⮱䔶᠖ᕔ喑Ꭳजλ 30 min ڲ ࢎ䗐䰶㉍℁㞟⩌➖эᙌக喍B喎 [52]
۳ౝᷭ≸ܧ➈ຣ͚ࢎ䗐䰶㉍⮱क़䛼ȡCUI ぶ [49] 䃫 Fig. 5 Schematic diagram of colorimetric biosensor for the
detection of kanamycin based on m-L-DNA probe
䃎γ͑͗ࣾᣏ䦵喍HP1 হ HP2喎Ƞ䔯䙺Ҁ-ᑂ➖ᱯ (A) [48] ; Schematic diagram of colorimetric biosensor
ϑࣹ䨫ᣏ䦵喍Ap-Pr喎Вࣷ䒲ߖᣏ䦵喑ౕⰛᴴ➖ࢎ䗐 for the detection of kanamycin based on DNA probe
䰶㉍⮱㼓̸ࣾ喑ᵥ䚥ᣏ䦵ࣾ⩌㐀ᲱअࡃࣷⰥο҉⩕喑 coupled with gold nanoparticles (B) [52]
䮼ऻᑂڒ䭽ݣᕔڲܴ䚣 Nt.AlwI ϔ⩌ࣹ䛺Ԏतᩫ๔ȡ ℁㞟⩌➖эᙌகᄦោ⩌㉍₸⪆⮱৺ᏁӬᢤȠⰡ
䄒эᙌக㗹ᮜԎतѻ喑キࢂȠᔘ䕌́᱙ѻ喑ᄦࢎ 㻯喑ѳ▢᩼Ꮣ᭜ധλᵥ䚥ᣏ䦵⮱ោ⩌㉍℁㞟эᙌக
䗐䰶㉍ᷭ≸ڤᰶ⾮ܧ⮱➦ᐯᕔহ▢᩼ᕔ喑ᷭ≸䭽ѻ ࣾᆂ⮱ڠ䩛ȡᑂڒᵥ䚥ᣏ䦵㜗㏱㷲Ԏतᩫ๔ゃ⪒В
㜠 14.7 pmol/Lȡ ࣷ㏠ㆠᣏ䦵᭜倅▢᩼Ꮣ⮱ᰶ᩵ᐼȡ㔰㭾ݝ䭲
э㐌⮱⩌㞟䄂ݯ䕇፥ႅౕ̺ज䛺ฺҬ⩕Ƞᭀऄ 丌৮ᵤ᱙⮱䷉㞟็ᵤᕔ喑ͧ䖬ٺឝ喑䰭ᵦᢛڤҀ
㗹ᮜ㉍ᒞ৺ぶ㑧◦喑ͧγ䔈̭ₒ倅℁㞟эᙌக ᗲۢ䃫䃎Ⱕ䔯Ꮑ⮱ᵥ䚥ᣏ䦵ࣷ℁㞟эᙌᷭ≸ᵵ喑
⮱ܳᲽᕔ㘪喑㘪์ϔ⩌䷉㞟अࡃ⮱㏠ㆠᱽ᫆Ꭼऄڠ हᬣ䛴⩕ᔘᢤȠᰶ᩵⮱ᵤ৮䶱ั⤳∂ȡₑใ喑ͧ
∕ȡڣ͚喑䛾㏠ㆠ䷄ㆿڣхᐯ⮱ᅭ䘕㶕䲏ぶ⻨ၽ 䖬ٺͨ㻯ռጛ喑℁㞟эᙌҀ㈨͚ϔ⩌⮱䷉㞟अࡃ䰭
Ҁڞᡜহ倅⊵ٶ㈨喑ࣷڣϻܳ᪐ݝ㖇䯳⟣ᔮϔ 㖁 ⩕ ᮧ㘪 ⩢ ၽ 䃫ิ喑 В 倅ڣ۳ ᕔ হज
⩌⮱ⰥᏁ䷉㞟喍ϻ䙿㏏ݝ㉘㞟喎হ॥ٶᏓअࡃ [50-51] 喑 䲍ᕔȡ
