Page 100 - 《精细化工》2023年第4期
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g786g ㏳ࡃጒ FINE CHEMICALS す 40 ࢤ
䭲ᵤ৮ᷭ≸͚⮱Ꮑ⩕ȡₑ喑ധλߌ㘪ࡃᵥ䚥ᣏ ቹڒ G-ఈ䨫Ҁϔ⩌⮱㢔ٶԎत䭺ѻ喑ϻ㔹ᐧ⿸㢔
䦵⮱ᬍ䚣䒲ߖԎतᩫ๔ឭᱜऄݝγᎬ∈ڠ∕ [29-31] 喑 ٶᑧᏓ̻ఈ⣜㉍⊀Ꮣ䬡⮱ڠ㈨ȡ䄒∂キӬᔘ䕌喑
ͧᲱᐧ⽠ჇȠោឝ⮱ោ⩌㉍₸⪆⩢ࡃ႓эᙌக ᷭ≸䭽ͧ 0.029 ȝg/mL喑̻́ڣЃ፥㻮ោ⩌㉍⮱ϑࣶ
ӈγᲮ๔⮱ӬݖȡHONG ぶ [32] ጔໆ䃫䃎䃳ݘᣏ䦵喑 ࣺᏁजᔪ⪒̺䃎喑䃮ᬻڣᄦఈ⣜㉍ᷭ≸ڤᰶ䒰ສ⮱
Ꭳౕ䒲ߖᣏ䦵⮱҉⩕̸⣝䲣ᴴᓗ⣜ݖ⩕喑हᬣ䃫 ▢᩼ᕔহ䔶᠖ᕔȡౕധλᵥ䚥ᣏ䦵⮱ោ⩌㉍₸⪆㢔
䃎ࣾᣏ䦵 H1 ̻ H2 ౕ䲣ᴴ㼓̸ࣾ䔈㵹䨫ᱯϑࣺ ٶэᙌᷭ≸͚喑ᵥ䚥ᣏ䦵̺ϲजВ҉ͧ䃳ݘٰУ喑
Ꮑϔ⩌Ԏतᩫ๔喍ఫ 3B喑ڣ͚喑S1ȠS2ȠS3 ͧ 䔅जВ҉ͧԎत䒙ᢏহᩫ๔ٰУ喑倅эᙌக⮱ܳ
ࢂ䨫ᵥ䚥ᣏ䦵喑H1ȠH2 ͧ͑ࣾ㐀Ჱ⮱ᵥ䚥ᣏ Ჽᕔ㘪ȡLUO ぶ [38] Вᵥ䚥䔯䙺Ҁ҉ͧ䃳ݘᣏ䦵喑䃫
䦵喎喑Ჱᐧγ̭ᬍ䚣ᬍᴴ䃝⮱⩢ࡃ႓эᙌகȡ䄒 䃎ο㶒 DNA ᣏ䦵҉ͧԎत䒙ᄩٰУ喑䃫䃎 Taqman
⩢ࡃ႓эᙌகౕᄦࢎ䗐䰶㉍⮱ᷭ≸͚㶕⣝ܧγ倅 ᣏ䦵ͧԎतᣏ䦵喑Ⱋᴴ➖̻䃳ݘᣏ䦵⮱➦ᐯᕔ㐀व
▢᩼ᕔহ➦ᐯᕔ喑ᷭ≸䭽ͧ 16 fmol/Lȡ̷ౕ䔝⩢ ᄩ㜡ο㶒 DNA ᣏ䦵̻Ԏतᣏ䦵ᒏᱯϑࣹ䨫喑䔈㔹
ࡃ႓эᙌக͚喑Y ಸ DNA ᣏ䦵হࣾᣏ䦵ڤᰶ ᑂܴࣾऐ䚣䒲ߖ⮱Ԏतᩫ๔ࣺᏁ喑⣝ᄦ⅕㟱㺬᳄
㐀Ჱ▢≨ᕔহᲱ䆎⽠Ⴧᕔ喑ౕⰛᴴ➖䃳ݘȠԎत㼓 ⮱倅➦ᐯᕔহ倅▢᩼ᕔᷭ≸ȡ
ࣾহព䲏ڤᰶ䛺㺮҉⩕ȡѳВ̷ᣏ䦵Ꮌ݄䃫䃎 э㐌㢔ٶധఏᴴ䃝䕇፥ႅౕ⽠ჇᕔጛȠ▢᩼Ꮣ
䒰ฺᱯ喑ज㘪ϔ⩌㗹ᮜԎत倅Ƞմ䭠ᕔぶ䬛䷅ȡͧ ѻȠᷭ≸㗹ᮜฺᱯぶ㑧◦ [39] 喑ݖ⩕㏠ㆠᱽ᫆⠙➦⮱
ₑ喑ⵁ⾣㔲Й㜡߈λхࡃ䃫䃎ᵥ䚥ᣏ䦵Ꮌ݄Ƞᐭࣾ 㢔ٶᕔ䉕Ჱᐧฺवᱽ᫆ᵥ䚥ᣏ䦵जВᰶ᩵ٸэ㐌
℁⢴ಸ⩢ࡃ႓эᙌக喑ᝃౕ⩢ࡃ႓эᙌக͚ᑂڒ็ 㢔ٶэᙌக⮱㑧◦喑ϻ㔹倅эᙌகౕक़ฺᱯധ䉕
䛺ᵥ䚣ᝃᬍ䚣䒲ߖԎतᩫ๔ゃ⪒ȠᲱᐧⷮᕔᵥ䚥ᣏ ᵤ৮͚⮱⽠Ⴧᕔহज䛺ฺᕔȡౕധλᵥ䚥ᣏ䦵⮱ោ
䦵Ꭳ㐀वⷮ⤍ܳ⻨ゃ⪒喑В䭺ѻ㗹ᮜԎतឝহۼ ⩌㉍㢔ٶ⩌➖эᙌᷭ≸͚喑䛼ၽ◦Ƞ̷䒙ᢏ㏠ㆠ䷄
ᄾմ䭠ᕔԎतȡ ㆿぶᬍȠ⽠ჇȠ⩌➖Ⱕღᕔສ⮱㏠ㆠᱽ᫆㷘Ꭼ∈Ꮑ
⩕ [40-41] ȡHE ぶ [42] Вᵥ䚥䔯䙺Ҁ҉ͧ䃳ݘᣏ䦵喑㐀व
2 ധλᵥ䚥ᣏ䦵⮱ᷭ≸ោ⩌㉍₸⪆㢔ٶ⩌ ⴠ☜䛼ၽ◦হ⅔ࡃⴠ☜⮱⠙➦ᕔ䉕喑Ჱᐧγ̭
➖эᙌக ڠ-ᐭ㢔ٶ∂ᷭ≸⸧㘧ι⩟ણ⮱㢔ٶ⩌➖эᙌக喍ఫ
4B喎ȡ䄒∂ݖ⩕γⴠ☜䛼ၽ◦⾮ܧ⮱㢔ٶ➦ᕔ喑
㢔ٶ⩌➖эᙌக᭜ᠴᄳڤᰶ㢔ٶ᩵Ꮑ⮱ᴴ䃝➖ हᬣВ⅔ࡃⴠ☜҉ͧ㢔ٶ⡊▚ݯ喑䭺ѻγ侹㗹ᮜ
䕇䓴䲆⩢॥䭱҉⩕ȠڞФ҉⩕Вࣷϟহ㉍-⩌➖㉍ぶ Ԏत喑倅γэᙌக⮱▢᩼ᏓȡLIU ぶ [43] 䃫䃎ᵥ䚥
㈨㐌҉⩕֣㖁λܳၽ䃳ݘᅯ̷喑Ꭳ́ᵦᢛࣾ⩌⮱ ᣏ䦵Ꭳ̷̻䒙ᢏ㏠ㆠ䷄ㆿ⮱ࣾٶ➦ᕔⰥ㐀व喑Ჱᐧ
㢔ٶᑧᏓअࡃᄦ䲣ᴴ䔈㵹ჇᕔჇ䛼ᷭ≸⮱⩌➖эᙌ γ̭㢔ٶ⩌➖эᙌக⩕λ⸧㘧ι⩟⅔ણ⮱ᷭ≸喑
க [33] ȡ㢔ٶэᙌகڤᰶ̺ऄ⍖ᏓȠ⅁Ҁぶ㉍ᒞ৺喑 䄒эᙌகᄦ⸧㘧ι⩟⅔ણ⮱ᷭ≸䭽ѻ㜠 0.11 ng/mL喑
Ҭ⩕ᄬপ䪬喑ᕔФ℁倅ぶх߬ [34-35] ȡᄳڤᰶ➦₷ߌ ᎣߌᏁ⩕λ䭲冩ᵤ᱙⮱ᷭ≸͚ȡ
㘪⮱ᵥ䚥ᣏ䦵㲺ڒ㢔ٶ⩌➖эᙌக⮱䃫䃎͚喑जᐭ
ࣾោ⩌㉍₸⪆倅᩵ᷭ≸∂ȡ
ധλᵥ䚥ᣏ䦵Ჱᐧោ⩌㉍₸⪆㢔ٶэᙌக喑ज
Вݖ⩕䲣ᴴ㼓ࣾ⮱ᣏ䦵㐀Ჱ䒙अᑂࣾ㢔ٶᑧᏓ⮱अ
ࡃ喑ϻ㔹ᄦⰛᴴោ⩌㉍䔈㵹ᷭ≸ܳᲽȡDEHGHANI
ぶ [36] ധλࣹ䨫 DNA ᣏ䦵ᄦϸႁι⅔ࡃ㏠ㆠ䷄ㆿ
⮱ᄮ〜҉⩕喑Ჱᐧγ̭ᬍᴴ䃝⮱㢔ٶэᙌக喑⩕
λᷭ≸ࢎ䗐䰶㉍ȡⰛᴴ➖ࢎ䗐䰶㉍⮱ႅౕᄩ㜡ࣹ䨫
DNA ᣏ䦵ܳ⻨ͧࢂ䨫喑Ҭᓄٲι⅔ࡃᓛႁ⮱㢔
ٶധఏ喍㒄ͦᬻ B喎ᓄݝ䛷ᩫ喑ϻ㔹Ҭ㢔ٶᑧᏓࣾ
⩌अࡃȡౕᰭ䔯У̸喑䄒эᙌகᄦࢎ䗐䰶㉍⮱㏬ᕔ
ᷭ≸㠰డͧ 24.75~137.73 nmol/L喑ᷭ≸䭽ͧ 7.5 nmol/Lȡ ఫ 4 ധλ G-ఈ䨫Ҁᵥ䚥ᣏ䦵⮱ఈ⣜㉍㢔ٶ⩌➖эᙌக
SUN ぶ [37] В G-ఈ䨫Ҁ㐀Ჱᵥ䚥䔯䙺Ҁͧ䃳ݘᣏ䦵喑 ᘼఫ喍A喎 [37] 喠ധλᵥ䚥ᣏ䦵֣㖁ⴠ☜㏠ㆠᱽ
Вநਾᾆͧ㢔ٶᴀ᫆喑Ჱᐧγ̭ᬍᴴ䃝⮱㢔ٶэ ᫆⮱⸧㘧ι⩟ણ㢔ٶ⩌➖эᙌகᘼఫ喍B喎 [42]
ᙌக喍ఫ 4A喎喑⣝γᄦఈ⣜㉍⮱倅᩵ᷭ≸ȡ䃳ݘ Fig. 4 Schematic diagrams of fluorescence biosensors for
ᣏ䦵̻䲣ᴴఈ⣜㉍䬡⮱➦ᐯᕔ䃳ݘ̻㐀वҬᓄᣏ the detection of tetracycline based on G-quadruplex
[37]
probe (A) and sulfamethazine based on nucleic acid
䦵⮱ G-ఈ䨫Ҁ㐀Ჱࣾ⩌अࡃ喑ᄩ㜡㢔ٶᴀ᫆நਾᾆ probe coupled with graphene nanomaterial (B) [42]