Page 102 - 《精细化工》2023年第4期

g788g ㇫㏳ࡃጒ FINE CHEMICALS す 40 ࢤ

4 ᵥ䚥ᣏ䦵ౕڣЃ፥㻮ᷭ≸ោ⩌㉍₸⪆⩌ 㶕䲏෋ᑧ៶ᰩ᪐ᄱэᙌக喑⩕λࢎ䗐䰶㉍⮱倅▢᩼
➖эᙌக͚⮱Ꮑ⩕ ᕔহ倅➦ᐯᕔᷭ≸ȡԛ亝λ㏠ㆠ䷄ㆿ̷⮱ DNA ࣹ䨫
ᣏ䦵➦ᐯᕔ䃳ݘࢎ䗐䰶㉍ऻ喑ፓᰶ៶ᰩ᪐ᄱԎतܳ
ᗙ㛯ᐼэᙌக᭜ݖ⩕Ⱋᴴ➖̻䃳ݘܳၽ㐀वऻ喑 ၽ Cy3 ⮱䔯䙺Ҁο㶒ᣏ䦵㷘䛷ᩫ喑ϻ㔹ᵦᢛ䓀ܧ⮱
ߌ㘪ࡃ⮱̻䲋ߌ㘪ࡃ⮱ᗙ㛯͸䬡ϔ⩌⮱㶕䲏Ꮑ߈ጛᐯ ៶ᰩ᪐ᄱ৺Ꮑᄦࢎ䗐䰶㉍䔈㵹Ⴧ䛼ܳᲽȡ᝭Ჱᐧ⮱
–7
Ҭᓄᗙ㛯ᑜᰟ喑Ꭳ䕇䓴⯾≸ϻᗙ㛯ᶮࣺᄱݝѺ㒛᩼ᙌ эᙌகᄦࢎ䗐䰶㉍ౕ 1.0×10 ~1.0×10 –11 g/mL ⮱㏬
ᣏ≸க̷⮱⓭ٶ᲌⮱Ѻ㒛Გ⶛Ⴧᗙ㛯ᶮ⮱अᒏ䛼ȡ҉ ᕔ㠰డڲ㶕⣝ܧ㞜ສ⮱㏬ᕔڠ㈨喑ٸ᰺γэ㐌ᷭ≸
̭ͧ⻺ᬍᴴ䃝⮱эᙌக喑ᗙ㛯ᐼэᙌகڤᰶᷭ≸䭽ѻȠ ᫦∂᧺҉㍮⤽Ƞ㕄ᬣ䪬ぶ㑧◦喑ᎣजᏁ⩕λ჋䭲➈
᜽᱙ѻȠܳᲽ䕌ᏓᔘȠᭀλ᧺҉ぶх߬ [54] ȡᄳߌ㘪হ ຣᵤ᱙͚ࢎ䗐䰶㉍⮱ᷭ≸ܳᲽȡ
㐀Ჱᕔ䉕͝ჹ็ᵤ⮱ᵥ䚥ᣏ䦵̻ᗙ㛯ᐼэᙌகⰥ㐀
व喑जВ᣽倅ᗙ㛯ᐼэᙌக⮱䔶᠖ᕔȠ▢᩼ᏓぶܳᲽ
ᕔ㘪ȡ఍ₑ喑ധλᵥ䚥ᣏ䦵⮱ᗙ㛯ᐼэᙌகౕោ⩌㉍
ᷭ≸䶳ഌᰶᒵ๔⮱ࣾᆂ⒉߈ȡHOU ぶ [55] Ჱᐧγ̭⻺В
ᵥ䚥䔯䙺Ҁ DNA ͧ䃳ݘᣏ䦵⮱ᓛಸᗙ㛯䭢݄喑⩕λ
Ⴧ䛼ܳᲽౌ䰶㉍喍ఫ 6喑ڣ͚喑OTC ͧౌ䰶㉍喎ȡ䃳ݘ
ᣏ䦵̻ౌ䰶㉍➦ᐯᕔ㐀वऻ喑ߌ㘪ࡃᗙ㛯఍ͧ㶕䲏Ꮑ
߈⮱अࡃ㔹ࣾ⩌ᑜᰟ喑ᗙ㛯⮱ռ䒙፲Ꮣ̻ౌ䰶㉍⊀Ꮣ
ౕ 1.0~100 nmol/L 㠰డڲႅౕ㞜ສ⮱㏬ᕔڠ㈨喑́䄒 ఫ 6 ധλᵥ䚥ᣏ䦵⮱ౌ䰶㉍ᗙ㛯ᐼэᙌக⹧ᘼఫ [55]
эᙌக⮱ᷭ≸䭽ͧ 0.2 nmol/LȡBAI ぶ [56] Вᵥ䚥䔯䙺 Fig. 6 Schematic diagram of cantilever array sensor for the
detection of oxytetracycline based on nucleic acid
Ҁ䃫䃎䃳ݘᣏ䦵Ჱᐧγᓛಸᗙ㛯䭢݄эᙌக喑჋⣝γ probe [55]
ᄦࢎ䗐䰶㉍⮱倅▢᩼ᕔহ倅➦ᐯᕔᷭ≸ȡ
㶕䲏෋ᑧ៶ᰩ᪐ᄱэᙌக᭜ᄳᒲ≸ܳၽ॥䭱λ ̷ౕ䔝͑ㆨោ⩌㉍₸⪆⩌➖эᙌக͚喑᝭䓽⩕
䛾ᆋ㶕䲏ϔ⩌ᅭ䘕㶕䲏ぶ⻨ၽڞᡜ᩵ᏁᎣӰҬ៶ᰩ᪐ ⮱ᵥ䚥ᣏ䦵ᅭ䭽λᵥ䚥䔯䙺Ҁ喑ڣЃㆨಸᵥ䚥ᣏ䦵
ᄱԎत෋ᑧ⮱̭⻺ܳᲽឭᱜ喑ڣ఍ڤᰶ▢᩼Ꮣ倅Ƞ➦ ⊶ࣷ䒰ᄾ喑ᷭ≸▢᩼Ꮣ䒰ѻȡхࡃ䃫䃎็ߌ㘪䯳᜽
ᐯᕔ倅Ƞᵤ৮ݣิキࢂぶх߬㔹ऄݝᎬ∈ڠ∕ [57-58] ȡ ಸᵥ䚥ᣏ䦵᭜ज䔈̭ₒᣏ㉏⮱᫦ा喑ౕ᣽倅ᷭ≸ᕔ
JIANG ぶ [59] ݖ⩕ᵥ䚥䔯䙺Ҁ̻ڣο㶒Ꮌ݄䃫䃎 㘪⮱हᬣۼᄾᷭ≸ᬣ䬡হ᜽᱙ȡ̺हោ⩌㉍₸⪆ᷭ
DNA ࣹ䨫䃳ݘᣏ䦵喑㐀व Au@AgNPs Ჱᐧγ̭⻺ ≸᫦∂℁䒰㻮㶕 1ȡ

㶕 1 ̺हោ⩌㉍₸⪆ᷭ≸᫦∂℁䒰
Table 1 Comparison of different methods for antibiotics detection

᫦∂ Ⱋᴴ➖ ᷭ≸㠰డ ᷭ≸䭽
–6
⩢ࡃ႓эᙌக喍䔯䙺Ҁᣏ䦵喎 [20] ఈ⣜㉍ 1.0×10 –14 ~1.0×10 mol/L 3.8×10 –15 mol/L
⩢ࡃ႓эᙌக喍ࣾ๦ᣏ䦵喎 [28] ࢎ䗐䰶㉍ 0.005~100 pmol/L 1.3 fmol/L
㢔ٶэᙌக喍ࣹ䨫 DNA ᣏ䦵喎 [36] ࢎ䗐䰶㉍ 24.75~137.73 nmol/L 7.5 nmol/L
㢔ٶэᙌக喍G-ఈ䨫Ҁᣏ䦵喎 [37] ఈ⣜㉍ 0.05~100 ȝg/mL 0.029 ȝg/mL
℁㞟эᙌக喍m-L-DNA ᣏ䦵喎 [48] ࢎ䗐䰶㉍ 0.0005~30 ng/mL 0.2 pg/mL
℁㞟эᙌக喍̶㳧᫸ܳၽᐭڠᣏ䦵喎 [53] ఈ⣜㉍ 0.3~10 nmol/L 266 pmol/L
倅᩵⋟Ⱕ㞟䅞∂ [60] ⅜䰶㉍Ƞఈ⣜㉍ 10~600 nmol/L 3.02Ƞ3.52 nmol/L
⋟Ⱕ㞟䅞-͟㖁䉕䅞∂ [61] 36 ⻺̺हោ⩌㉍ 0.3~27.6 ȝg/kg 0.1~9.2 ȝg/kg
ٺ⫘ܳᲽ∂ [62] ⸧㘧ι⩟୔ણ 0.033~33 ng/mL 0.026 ng/mL

㘪⮱็ᵤᕔВࣷᭀλव᜽Ƞԛ亝হᩦ䕍ぶ➦◦喑Ꭼ
5 㐀᲌䄚̻ᆂ᱈
∈Ꮑ⩕λ⩌➖эᙌக⮱ᐭࣾⵁ⾣͚ȡ䓾ᎡᲒ喑䮼Ɑ

ᷭ≸丌৮͚ោ⩌㉍₸⪆ⅡᎠᄦԊ䯉ϧㆨ֒Ꮴহ ܳၽ䃳ݘȠ⩹䲏㏱㷲ȠԎतᩫ๔Ƞ㏠ㆠᣏ䦵ぶⰥڠ
Ⴖڕڤᰶ䛺㺮ᘼͶ喑⩌➖эᙌக̻э㐌ܳᲽ᫦∂Ⱕ ឭᱜ⮱̺᫚䔈ₒ喑ധλᵥ䚥ᣏ䦵⮱⩌➖эᙌக㣤ᓄ
℁ౕោ⩌㉍₸⪆ᷭ≸䶳ഌڤᰶ䒰๔х߬হ⒉߈ȡᵥ γᔘ䕌ࣾᆂ喑ͧោ⩌㉍₸⪆⮱倅᩵ᷭ≸ፓᲒγ᫝ᱧ
䚥ᣏ䦵҉̭ͧ⻺᫝ಸ⩌➖ܳᲽጒڤ喑ڤᰶ㐀Ჱহߌ 䕴ȡ᱙᪴ͨ㺮ϻ⩢ࡃ႓⩌➖эᙌகȠ㢔ٶ⩌➖эᙌ