Page 99 - 《精细化工》2023年第4期

す 4 ᱌ ䷉⢶ྤ喑ぶ: ᵥ䚥ᣏ䦵ౕោ⩌㉍₸⪆⩌➖эᙌᷭ≸͚⮱Ꮑ⩕ g785g

Ꭳౕ჋䭲ڪ㢜হ➈ຣᵤ᱙ܳᲽ͚ᓄݝγ侹䃮ȡ䮑ₑ
Вใ喑ധλᵥ䚥ᣏ䦵⮱ោ⩌㉍⩢ࡃ႓эᙌக፥⩕⮱⩢
[19]
ࡃ႓ܳᲽ᫦∂䔅ᰶ⩢ࡃ႓䭨ោ∂ ȡJAHANBANI ぶ [20]
䕇䓴ᄦఈ⣜㉍ࢂ䨫ᵥ䚥䔯䙺Ҁ䔈㵹ᩦᕔ䃫䃎ᵥ䚥ᣏ
䦵喑ᩦᕔऻ⮱ᵥ䚥䔯䙺Ҁᣏ䦵̻Ⱋᴴ➖ఈ⣜㉍➦ᐯ
ᕔ㐀वऻࣾ⩌ⰥᏁ⮱㐀Ჱअࡃ喑䭨ⶺγ⩢ၽ⮱䒙⼨喑
ϻ㔹ϔ⩌⩢䭨ԎतअࡃВ჋⣝ᄦఈ⣜㉍⮱ᷭ≸喑э
–15
ᙌக⮱ᷭ≸䭽ѻ㜠 3.8×10 mol/Lȡౕധλ̷䔝ऱ⩢
ࡃ႓ܳᲽ᫦∂Ჱᐧ⮱⩢ࡃ႓эᙌக͚喑ᵥ䚥䔯䙺Ҁ
ᣏ䦵҉ͧ䃳ݘٰУࣾᡒγ䛺㺮҉⩕ȡᵥ䚥䔯䙺Ҁᣏ
䦵̻Ⱋᴴោ⩌㉍͸䬡⮱➦ᐯᕔ䃳ݘ҉⩕ᄳ㼓ࣾᵥ䚥
䔯䙺Ҁᣏ䦵ܳၽᲱಸࣾ⩌अࡃᝃᄩ㜡ࣹᣏ䦵ܳ⻨喑
䔈㔹キࢂౝ䕇䓴ᑂڒ⩢ࡃ႓ܳᲽឭᱜ䔈㵹㶕ᒮ喑В

჋⣝Ⱋᴴ➖⮱ᷭ≸ȡѳ䔆⻺ࢂ㏜⮱Ჱಸअࡃᝃࣹᣏ ఫ 3 ധλ็ᵥ䚥ᣏ䦵ࡼह҉⩕⮱⅜䰶㉍⩢ࡃ႓⩌➖э
䦵ܳ⻨ϔ⩌⮱ᷭ≸▢᩼Ꮣᒭᒭᰶ䭽喑䰭ᑂڒԎतᩫ ᙌக⹧ᘼఫ喍A喎 [17] 喠Ⱋᴴោ⩌㉍㼓ࣾᵥ䚥ᣏ䦵䨫
๔ឭᱜ喑ຯധλᵥ䚥⮱Ԏतᩫ๔Ƞ䚣יࡃԎतᩫ๔ ᐼᱯϑࣺᏁ⹧ᘼఫ喍B喎 [32]
ᝃ㏠ㆠᱽ᫆Ԏतᩫ๔喑ϻ㔹䓫ݝ倅᩵Ƞ倅▢᩼ᷭ≸ Fig. 3 Schematic diagram of electrochemical biosensor for
the detection of chloramphenicol based on synergistic
⮱Ⱋ⮱ȡओ̭᫦䲏喑ϧጒҀใよ䔶ឭᱜ㣤ᓄ⮱ᵥ䚥 action of nucleic acid probe (A) [17] ; Schematic diagram
䔯䙺Ҁज㘪ႅౕϟহ߈ѻ⮱㑧䮤喑䔅䰭ᩦ䔈Ҁใよ䔶 of target antibiotic-triggered chain hybridization
ឭᱜ喑᣽倅ᵥ䚥䔯䙺ҀᄦⰛᴴោ⩌㉍⮱ϟহ߈ȡ䓾Ꭱ reaction of nucleic acid probe (B) [32]

Გ喑పڲใⵁ⾣㔲㜡߈λᩦ䔈э㐌ᠴ᪝ჹ䯳⮱䙺Ҁ㈨ ͧγ䔈̭ₒ᣽倅ោ⩌㉍⩢ࡃ႓эᙌக⮱ܳᲽᕔ
㐌䔈ࡃ喍SELEX喎ឭᱜ喑ຯ䛴⩕䭡ᕔ SELEXȠࣺा 㘪喑䕇፥䛴⩕⮱᫦∂ᰶВ̸͑⻺ȡ̭⻺᭜ᑂڒڤᰶ
SELEX ぶឭᱜ喑ᑂڒ͇̀ܳ⻨ឭᱜ喍≮ᐼ㏳㘋ᱜȠᓛ ⠙➦⮱ٶ႓Ƞ⩢ⷮ႓হࡃ႓ࣺᏁᕔ㘪⮱㏠ㆠᱽ᫆喑
≮ᣔឭᱜȠࣹᵥぶ⻨ၽ㶕䲏ڞᡜឭᱜぶ喎ȠҬ⩕ٵ䔈㖇 ຯ䛾ᆋࣷ䛾ᆋ⅔ࡃ➖㏠ㆠᱽ᫆Ƞⷠࣷ⅛ᣧᱯⷠ㏠ㆠᱽ
व䚣䨫ᐼࣺᏁ喍PCR喎ឭᱜ喍Π⋟ PCRȠ⋟␡ᐼ᪝ [21-24]
᫆Ƞᄩ⩢㖇व➖㏠ㆠᱽ᫆ぶ ȡฺव㏠ㆠᱽ᫆ݣิ
ႄ PCR喎Ƞ㐀व倅䕇䛼≸Ꮌ䓴⼸喑๔๔ᩦ઱γᵥ䚥䔯
⮱ߌ㘪ࡃᵥ䚥ᣏ䦵⩌➖Ⱕღᕔ倅Ƞ⽠Ⴧᕔສ喑ᰶݖ
䙺Ҁᕔ㘪ȡₑใ喑》ζ䔶᠖⮱䙺Ҁ㈨㐌䔈ࡃឭᱜጟ
λ᣽倅⩢ࡃ႓⩌➖эᙌக⮱⽠ჇᕔȠज䛺ฺᕔࣷ۳
㏼㷘᜽ߌᐭࣾȡ๔䛼ⵁ⾣㶕ᬻ喑䔆⻺よ䔶ឭᱜ㣤ᓄ
⶛ᕔ喑Ꭳᰶ᱈Ꮑ⩕λฺᱯ჋䭲ᵤ৮ᷭ≸ȡ䛾ᆋᰶᱧ
⮱ᵥ䚥䔯䙺Ҁ̻䲣ᴴ⮱ϟহ߈倅λэ㐌 SELEX よ
ᵳ᳣喍MOF喎҉̭ͧ⻺ڤᰶ⠙➦➖⤳ࡃ႓ᕔ䉕⮱᫝
䔶ឭᱜ喑Ꭳౕ჋䭲Ꮑ⩕͚ᓄݝ侹䃮ȡѳ᭜̷䔝ឭᱜ
ಸ㏠ㆠᱽ᫆ [25-26] 喑ͧោ⩌㉍₸⪆⩢ࡃ႓эᙌக⮱Ჱᐧ
Ꮑ⩕㠰డ䒰⾱喑ຯ҂ᰶ᩵Ꮑ⩕λោ⩌㉍ぶᄼܳၽ䶳
᣽ӈγᰶ߈ᩜᠮȡCHEN ぶ [27] ധλ Y ಸ DNA ᣏ䦵㖁
ഌϺ䰭䔈̭ₒⵁ⾣ȡౕᩦ䔈Ҁใよ䔶ឭᱜ⮱हᬣ喑ज
व MOF ҉ͧ㏠ㆠԎतᴴ䃝喑Ჱᐧγ̭⻺᫝ಸ็⻺ោ
䕇䓴ᄦᵥ䚥䔯䙺ҀᎼ݄䔈㵹ᩦᕔВ䃫䃎็͗㐀वѺ
⩌㉍हᬣᷭ≸⮱⩢ࡃ႓эᙌக喑჋⣝γᄦ⅜䰶㉍হ
◦⮱็Фᵥ䚥䔯䙺Ҁᣏ䦵喑᣽倅ڣᄦⰛᴴោ⩌㉍⮱➦
ౌ䰶㉍⮱倅᩵ᷭ≸喑ᷭ≸䭽ܳݘͧ 33 হ 48 fmol/Lȡ
ᐯᕔহϟহ߈ȡ
ₑใ喑ᑂڒԎतᩫ๔ឭᱜजВᰶ᩵᣽倅⩢ࡃ႓эᙌ
க⮱ܳᲽᕔ㘪ȡWANG ぶ [28] 䃫䃎็⻺ DNA ࣾ๦ᣏ
䦵ࣷࢂ䨫䒲ߖᣏ䦵喑㖁व Phi29 㖇व䚣䒲ߖ⮱䲣ᴴ
ᓗ⣜ព෋হᵥ䚥ใܴ䚣ċ喍Exoċ喎䒲ߖ⮱⁎⩌䲣
ᴴᓗ⣜ゃ⪒喑Ჱᐧγᷭ≸ࢎ䗐䰶㉍⮱⩢ࡃ႓эᙌ
கȡ䄒᫦∂ጔໆ䃫䃎ᵥ䚥ᣏ䦵㐀व䚣䒲ߖ⮱็䛺ᓗ
⣜Ԏतᩫ๔喑ݖ⩕ Phi29 㖇व䚣Ƞ䭽ݣᕔڲܴ䚣
Nt.AlwIȠExoċ䚣⮱➦ᕔ喑჋⣝γ䲣ᴴহ⁎⩌䲣ᴴ

ఫ 2 ധλ̺ह⩢ࡃ႓ܳᲽ᫦∂⮱ᷭ≸ោ⩌㉍₸⪆⮱⩌ ⮱ᓗ⣜ݖ⩕喑ϻ㔹䓫ݝγ็䛺Ԏतᩫ๔⮱᩵᳉喑᣽
➖эᙌக⹧ᘼఫ 倅γᷭ≸⮱▢᩼Ꮣ喑हᬣᰡキӬᔘ䕌ȡ⩞λ䚣⮱Ф
Fig. 2 Schematic diagram of biosensors for the detection ᵩ℁䒰ᬯ䉢喑ݣิ䓴⼸ฺᱯ喑́≨ᕔᭀऄ⣜ධ⮱ᒞ
of antibiotic residues based on different electrochemical
analysis methods ৺喑䭽ݣγധλ䚣䒲ߖԎतᩫ๔⮱⩢ࡃ႓эᙌகౕ