Page 97 - 《精细化工》2023年第4期

す 40 ࢤす 4 ᱌ ㇫ ㏳ ࡃ ጒ Vol.40, No.4
20 23 Ꭱ 4 ᰵ FINE CHEMICALS Apr. 2023

㐩䃧
ᵥ䚥ᣏ䦵ౕោ⩌㉍₸⪆⩌➖эᙌᷭ≸͚⮱Ꮑ⩕

*
䷉⢶ྤ喑⢸ Ϋ喑ক㟠㟠喑叱 ᗓ
喍ࢄϙ᳄͇๔႓ 䒨ጒ̻丌৮႓䮏喑↌㟼 ࢄϙ 210037喎

ᦅ㺮喟ោ⩌㉍҉ͧߕ➖⇨⫄ݯহ⩌䪬Ӱ䔈ݯᎬ∈Ꮑ⩕λۉ͇Ƞ⪉➔͇ȠⅡϔ৮ڨ⃃͇喑ᄩ㜡ߕḺ➖丌৮͚ោ⩌
㉍₸⪆䛼䊲ᴴ喑͒䛺༮㗮ϧҀ֒Ꮴȡ఍ₑ喑ᷭ≸丌৮͚ោ⩌㉍₸⪆ڤᰶ䛺㺮ᘼͶȡ㔹⣝ᰶ⮱ោ⩌㉍₸⪆ᷭ≸᫦
∂ຯᓛ⩌➖∂Ƞٺ⫘႓ܳᲽȠ⋟Ⱕ㞟䅞-䉕䅞∂Ƞ℈㏳ノ⩢∠ぶ喑䕇፥ڤᰶ㕄ᬣ䪬Ƞ᧺҉ฺᱯȠ᜽᱙倅ぶ㑧◦ȡ
⩌➖эᙌக҉̭ͧ⻺倅᫝ឭᱜ喑ڤᰶᔘ䕌キࢂȠ▢᩼Ꮣ倅Ƞ䔶᠖ᕔສȠ᜽᱙ѻぶ➦◦喑ౕោ⩌㉍₸⪆ᷭ≸䶳ഌ
ڤᰶ䒰๔х߬ȡᵥ䚥ᣏ䦵҉̭ͧ⻺᫝ಸ⩌➖ܳᲽጒڤᎬ∈Ꮑ⩕λ⩌➖эᙌக⮱ᐭ͚ࣾ喑ᄳڣᑂڒោ⩌㉍₸⪆⮱
⩌➖эᙌᷭ≸ͧ჋⣝ោ⩌㉍₸⪆⮱倅᩵ᷭ≸ᐭ䓌γ᫝䕁ᒱȡ䄒᪴ϻ⩢ࡃ႓⩌➖эᙌகȠ㢔ٶ⩌➖эᙌகȠ℁㞟
⩌➖эᙌகВࣷڣЃ፥㻮⩌➖эᙌக᫦䲏㐩䔝γᵥ䚥ᣏ䦵ౕោ⩌㉍₸⪆⩌➖эᙌᷭ≸͚⮱Ꮑ⩕ⵁ⾣䔈ᆂ喑Ꭳᆂ
᱈γ䄒䶳ഌ᱗Გ⮱ࣾᆂݺᮜȡ
ڠ䩛䃺喟ោ⩌㉍喠₸⪆喠ᵥ䚥ᣏ䦵喠ᷭ≸喠⩌➖эᙌக
͚ఫܳㆨत喟TS207.5喠TP212.3 ᪴⡛ᴴ䃳⴮喟A ᪴」㑃त喟1003-5214 (2023) 04-0783-08

Application of nucleic acid probes in biosensing
detection of antibiotic residues

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YAN Yuting, WANG Qian, ZHOU Fangfang, HUANG Yue
喍College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu, China喎

Abstract: Wide usage of antibiotics, as animal therapeutic agents and growth promoters, in agriculture,
animal husbandry and aquatic product breeding lead to excessive antibiotic residues in animal and plant
foods, which seriously threatens human health. Therefore, detection of antibiotic residues in food is of great
significance. However, the existing detection methods, such as microbiological assay, enzyme-linked
immunoassay, liquid chromatography-mass spectrometry, and capillary electrophoresis, usually have the
disadvantages of time consuming, complex operation and high cost. Biosensors, a new technology with the
advantages of being rapid and simple, high sensitivity, good selectivity and low cost, exhibit great potential
for application in the field of antibiotic residue detection. Nucleic acid probe, a new tool for biological
analysis, has been widely used in the biosensor development. Nucleic acid probe introduced for the
biosensing detection of antibiotic residues opens up a new way for efficient detection. Herein, the
application of nucleic acid probe in the biosensing detection of antibiotic residue is reviewed from the
aspects of electrochemical biosensor, fluorescence biosensor, colorimetric biosensor and other common
biosensors, and the future development directions are discussed.
Key words: antibiotic; residue; nucleic acid probe; detection; biosensor

ោ⩌㉍᭜ᓛ⩌➖ౕА䅏≨ߕ͚ϔ⩌⮱⁎㏔А䅏 ⮱␒⩕喑ᄩ㜡ڣౕߕḺ➖ᕔ丌৮͚₸⪆䊲ᴴ喑䔈㔹
[1]
[3]
ϔ➖ᝃ㔲ϧጒव᜽⮱ㆨѩ➖ 喑఍ڣڤᰶោ㣹≨ᕔ 䕇䓴丌➖䨫⮱ჹ䯳᩵Ꮑ࢞რϧҀ֒Ꮴ ȡ఍ₑ喑͒
ᑧȠោ㣹䅞ᎬȠ҉⩕䓲䕌ぶх◦ [2-3] 喑Ꭼ∈Ꮑ⩕λۉ ᵩᣔݣោ⩌㉍₸⪆ⅡᎠ喑ᄦԊ䯉ϧㆨ䏘Ҁ֒ᏤহႶ
͇Ƞ⪉➔͇ȠⅡϔڨ⃃͇ぶ䶳ഌȡѳ᭜⩞λោ⩌㉍ ڕ㜠ڠ䛺㺮ȡ፥㻱⮱ោ⩌㉍ᷭ≸᫦∂ຯ倅᩵⋟Ⱕ㞟

ᩣ⽬ᬒ᱌喟2022-05-09; Ⴧ⩕ᬒ᱌喟2022-08-26; DOI: 10.13550/j.jxhg.20220448
ധ䛾䶦Ⱋ喟పუ㜗♣⻾႓ധ䛾喍31901771喎
҉㔲キϸ喟䷉⢶ྤ喍1998ÿ喎喑ຠ喑ⶂธ⩌ȡ㖁㈨ϧ喟叱 ᗓ喍1992ÿ喎喑ຠ喑䃟ጵ喑E-mail喟huangyue@njfu.edu.cnȡ