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第 35 卷第 8 期 精 细 化 工 Vol.35, No.8
201 8 年 8 月 FINE CHEMICALS Aug. 2018

医药与日化原料
疏水壳聚糖的制备及其凝血性能

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温华强 ，马家骅 ，王 静 ，李雨秋 ，朱峻杉 ，谭承佳
（1. 西南科技大学 四川省生物质资源利用与改性工程技术研究中心，四川 绵阳 621010；2. 绵阳师范
学院 生命科学与技术学院，四川 绵阳 621006）
摘要：为增强壳聚糖的止血效果，采用酰基化改性在壳聚糖氨基上连接长链烷基以形成疏水性壳聚糖。采用单
因素实验对反应温度、月桂酸酐的浓度进行筛选，以疏水壳聚糖的黏度、取代度及凝血效果作为评价指标，通
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过梯度设计筛选得到的最优工艺条件为：温度 55 ℃，壳聚糖质量分数为 1%，月桂酸酐浓度 7.84×10 mol/L。
采用红外、核磁共振、接触角测试仪对其进行了表征，表明合成了疏水壳聚糖。体外凝血实验结果表明：疏水
壳聚糖的止血效果与氨基的取代度在一定范围内呈正相关性，当取代度为 10.8%和 15.9%的疏水壳聚糖与血液分
别混合后（疏水壳聚糖在混合物中质量分数不小于 0.75%），1~4 s 左右可形成稳定的凝胶，从而凝血。又通过
小鼠肝脏止血实验发现疏水壳聚糖粉末能够迅速止血，且效果显著优于壳聚糖。
关键词：疏水壳聚糖；月桂酸酐；取代度；凝血；医药原料
中图分类号：TQ469 文献标识码：B 文章编号：1003-5214 (2018) 08-1370-06

Preparation of Hydrophobic Chitosan and Its Hemostasis

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WEN Hua-qiang , MA Jia-hua , WANG Jing , LI Yu-qiu , ZHU Jun-shan , TAN Cheng-jia
（1. Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province, Southwest
University of Science and Technology, Mianyang 621010, Sichuan, China; 2. Mianyang Normal University, College of
Life Science and Biotechnology,Mianyang 621006, Sichuan, China）
Abstract: To enhance the hemostatic effect of chitosan, a long chain alkyl group was connected to the
amino group of chitosan by acylation to form hydrophobic chitosan. Single factor experiments were used to
optimize the reaction temperature and the concentration of lauric anhydride, and intrinsic viscosity, degree
of substitution and coagulation effect of hydrophobic chitosan were used as evaluation indexes. The
optimum conditions for the preparation of hydrophobic chitosan were found as follows: reaction
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temperature 55 ℃, mass fraction of chitosan 1%, concentration of lauric anhydride 7.84×10 mol/L. Its
chemical structure and physical properties were studied by IR, NMR and contact angle measurement. It was
found that hydrophobic chitosan was successfully synthesized. The results of in vitro coagulation
experiments showed that the hemostatic effect of hydrophobic chitosan was positively correlated with its
degree of substitution in a certain range. The hydrophobic chitosan with 10.8% and 15.9% degree of
substitution was mixed with plasma, respectively. The mass fraction was no less than 0.75% in the mixture,
and it could forma stable gel in 1 to 4 s,whichexhibited an effect of blood coagulation. The mouse liver
hemostasis experimentrevealed that thehydrophobic chitosan powder had betterhemostatic effect than
chitosan.
Key words: hydrophobic chitosan; lauric anhydride; degree of substitution; blood clotting; drug materials
Foundation items: National Natural Science Foundation of China(30902005); Project of Science
&Technology Department of Sichuan Province (2017JY0163)

在战争、重大交通事故以及自然灾害等危急情 况下，因无法迅速止血而导致失血过多是受创致死

收稿日期：2018-03-05; 定用日期：2018-05-22; DOI: 10.13550/j.jxhg.20180145
基金项目：国家自然科学基金（30902005）；四川省科技厅项目（2017JY0163）
作者简介：温华强（1991—），男，硕士生。联系人：马家骅（1978—），男，副教授，电话：0816-6089531，E-mail：jiahuama@163.com。