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第 35 卷第 9 期 精 细 化 工 Vol.35, No.9
201 8 年 9 月 FINE CHEMICALS Sept. 2018

淀粉化学品
运载 β-胡萝卜素的 Pickering 乳液的制备及表征

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谢桂杰 ，梁 蓉 1,2* ，杨 成
（1. 江南大学 化学与材料工程学院，江苏 无锡 214122；2. 江南大学 合成与生物胶体教育部重点实验
室，江苏 无锡 214122）

摘要：为改善 β-胡萝卜素的水相溶解性及生物利用率，采用淀粉纳米晶（SNC）为颗粒乳化剂稳定 Pickering 乳
液，对 β-胡萝卜素进行乳化包埋。考察了油相体积分数和 β-胡萝卜素含量对运载 β-胡萝卜素的 Pickering 乳液储
藏稳定性的影响。结果显示，当 SNC 含量为 1%（以水相质量为基准），油相（溶有 β-胡萝卜素的辛酸/癸酸甘
油三酯）体积分数为 50%，β-胡萝卜素含量为 0.050%（以油相的质量为基准）时，采用含量为 0.80%（以水相
的质量为基准）的季铵盐壳聚糖（QCS）对 Pickering 乳液进行修饰后，乳液的乳析现象可明显改善，且储藏稳
定性显著提高。体外模拟消化实验结果显示，QCS 修饰后乳液中油脂的消化程度由 56.68%±1.56%提高到
71.70%±2.13%，β-胡萝卜素的生物可给率由 33.59%±0.82%提高到 57.62%±1.58%。采用 QCS 进行修饰不仅可以
提高 β-胡萝卜素 Pickering 乳液的稳定性，还可以提高乳液中油脂的消化程度和 β-胡萝卜素的生物可给率。
关键词：Pickering 乳液；β-胡萝卜素；淀粉纳米晶；季铵盐壳聚糖；体外消化；生物可给率；淀粉化学品
中图分类号：TS232 文献标识码：A 文章编号：1003-5214 (2018) 09-1582-07

Preparation and Characterization of Pickering
Emulsions Loaded with β-Carotene

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XIE Gui-jie , LIANG Rong 1.2* , YANG Cheng
（1. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China; 2. Key Laboratory of
Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China）

Abstract: In order to improve the solubility and bioavailability of β-carotene, the Pickering emulsions
prepared with starch nanocrystals (SNC) as particle emulsifier were used to emulsify and encapsulate
β-carotene. The factors affecting the storage stability of Pickering emulsion carrying β-carotene, such as
volume fraction of oil phase and mass fraction of β-carotene were investigated. The results showed that
when the content of SNC was 1% (based on the mass of aqueous phase), the volume fraction of oil phase
(caprylic/capric triglyceride containing β-carotene) was 50%, the content of β-carotene was 0.050% (based
on the mass of oil phase), the Pickering emulsion was modified with quaternary ammonium salt chitosan
(QCS) at a content of 0.80% (based on the mass of aqueous phase). The resultant Pickering emulsion
exhibited an obvious improvement in separation of emulsion and a significant increase in storage stability.
At the same time, the in vitro simulated digestion experiment showed that the digestion degree of the oil in
the emulsion after QCS modification was increased from 56.68%±1.56% to 71.70%±2.13%, and the
bioaccessibility of β-carotene was also increased from 33.59%±0.82% to 57.62%±1.58%. Therefore, QCS
modification can not only improve the stability of β-carotene Pickering emulsion, but also improve the
digestion degree of oil in the emulsion and the bioaccessibility of β-carotene.
Key words: Pickering emulsion; β-carotene; starch nanocrystals; quaternary ammonium chitosan; in vitro
digestion; bioaccessibility; starch chemicals
Foundation item: National Natural Science Foundation of China (31401533)

收稿日期：2017-08-25; 定用日期：2017-12-12; DOI: 10.13550/j.jxhg.20170694
基金项目：国家自然科学基金（31401533）
作者简介：谢桂杰（1993—），女，硕士生。联系人：梁 蓉（1983—），副教授，电话：0510-85917071，E-mail：rongliang@jiangnan.edu.cn。