Page 150 - 精细化工2019年第8期
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第 36 卷第 8 期 精 细 化 工 Vol.36, No.8
201 9 年 8 月 FINE CHEMICALS Aug. 2019
食品与饲料用化学品
不同前处理对动物组织中氯丙嗪残留检测对比
*
张 文,徐梓焓,鲁佳琦,谢宇恒,黄业传
(西南科技大学 生命科学与工程学院,四川 绵阳 621010)
摘要:采用超高效液相色谱串联质谱技术,比较 QuECHERS 法、MCX 固相萃取法和叔丁基甲醚直接提取法 3
种前处理方法对猪肉、猪肝、鸡肉、鱼肉中氯丙嗪残留检测效果的影响。结果表明:3 种方法对氯丙嗪的检出
限为 0.2 μg/kg,定量限为 0.5 μg/kg。在不同加标水平下,QuECHERS 法、MCX 固相萃取法和叔丁基甲醚直接
提取法处理的各类基质中加标回收率(72.5%~108.8%)和相对标准偏差(2.1%~8.6%)均满足方法学要求。MCX
固相萃取法净化效果优于 QuECHERS 法、叔丁基甲醚直接提取法。整体回收率从高到低依次是 MCX 固相萃取
法>QuECHERS 法>叔丁基甲醚直接提取法。对于脂肪含量较高的猪肉,3 种前处理方法回收率均略低于其他 3
种基质。
关键词:氯丙嗪;液相色谱;质谱;前处理;固相萃取;QuECHERS;叔丁基甲醚;饲料用化学品
中图分类号:O657.63;S859.84 文献标识码:A 文章编号:1003-5214 (2019) 08-1638-06
Detection of Chlorpromazine Residues in Animal Tissues by
Different Sample Preparation Methods
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ZHANG Wen, XU Zi-han, LU Jia-qi, XIE Yu-heng, HUANG Ye-chuan
(School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan,
China)
Abstract: Ultra performance liquid chromatography tandem mass spectrum/mass spectrum (UPLC-MS/MS)
was used to detect chlorpromazine residues in porcine muscle, liver, chicken and fish. Three sample
preparation methods including QuECHERS, MCX solid-phase extraction and direct extraction by tert-butyl
methyl ether were compared. The results showed that the limit of detection (LOD) of chlorpromazine by
three methods was 0.2 μg/kg, and the limit of quantitation (LOQ) was 0.5 μg/kg. At different addition levels,
the recovery and relative standard deviation (RSD) by three sample preparation methods were 72.5%~
108.8% and 2.1%~8.6%, respectively. All met the methodological requirements. The purifying effect of
MCX solid-phase extraction was better than the other sample preparation methods. The order of overall
recovery was from high to low: MCX solid-phase extraction, QuECHERS, and direct extraction by tert-
butyl methyl ether. The recoveries for pork with high fat content by three preparation methods were lower
than those of liver, chicken and fish.
Key words: chlorpromazine; LC; MS; sample preparation; solid-phase extraction; QuECHERS; tert-butyl
methyl ether; feedstuff chemicals
氯丙嗪(Chlorpromazine,CPZ),又称冬眠灵、 动物在过量食用药物后会造成体内药物残留 [4- 5] 。目
氯普马嗪等,是第一个吩噻嗪类抗精神病药 [1-2] 。同 前,许多国家和组织已针对各类兽药残留制定了相
时,氯丙嗪又是一类动物生长促进剂,可以起到使 应标准 [6-7] 。中国《动物性食品中兽药最高残留限量》
[3]
其嗜睡少动、加快生长和改变肉质的作用 。但是, (农业部 2002 年第 235 号公告)明确指出:氯丙嗪
收稿日期:2019-03-21; 定用日期:2019-05-08; DOI: 10.13550/j.jxhg.20190220
基金项目:四川省科技厅应用基础研究计划项目(2016JY0110);农业部农产品质量安全重点实验室开放课题(2018-KF-04);西南科
技大学龙山学术人才科研支持计划(17LZX546)
作者简介:张 文(1988—),男,博士,讲师,硕士生导师,E-mail:zhangwayne1988@163.com。联系人:黄业传(1975—),男,
博士,教授,硕士生导师,E-mail:hyc2005@ sina.com。