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第 36 卷第 8 期                             精   细   化   工                                  Vol.36, No.8
             201 9 年 8 月                             FINE CHEMICALS                                 Aug.    2019


              食品与饲料用化学品
                    不同前处理对动物组织中氯丙嗪残留检测对比



                                                                                       *
                                 张   文,徐梓焓,鲁佳琦,谢宇恒,黄业传
                                   (西南科技大学  生命科学与工程学院,四川  绵阳    621010)


                 摘要:采用超高效液相色谱串联质谱技术,比较 QuECHERS 法、MCX 固相萃取法和叔丁基甲醚直接提取法 3
                 种前处理方法对猪肉、猪肝、鸡肉、鱼肉中氯丙嗪残留检测效果的影响。结果表明:3 种方法对氯丙嗪的检出
                 限为 0.2 μg/kg,定量限为 0.5 μg/kg。在不同加标水平下,QuECHERS 法、MCX 固相萃取法和叔丁基甲醚直接
                 提取法处理的各类基质中加标回收率(72.5%~108.8%)和相对标准偏差(2.1%~8.6%)均满足方法学要求。MCX
                 固相萃取法净化效果优于 QuECHERS 法、叔丁基甲醚直接提取法。整体回收率从高到低依次是 MCX 固相萃取
                 法>QuECHERS 法>叔丁基甲醚直接提取法。对于脂肪含量较高的猪肉,3 种前处理方法回收率均略低于其他 3
                 种基质。
                 关键词:氯丙嗪;液相色谱;质谱;前处理;固相萃取;QuECHERS;叔丁基甲醚;饲料用化学品
                 中图分类号:O657.63;S859.84      文献标识码:A      文章编号:1003-5214 (2019) 08-1638-06


                         Detection of Chlorpromazine Residues in Animal Tissues by
                                     Different Sample Preparation Methods


                                                                                            *
                             ZHANG Wen, XU Zi-han, LU Jia-qi, XIE Yu-heng, HUANG Ye-chuan
                 (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan,
                 China)


                 Abstract: Ultra performance liquid chromatography tandem mass spectrum/mass spectrum (UPLC-MS/MS)
                 was  used  to  detect  chlorpromazine  residues  in  porcine  muscle,  liver,  chicken  and  fish.  Three  sample
                 preparation methods including QuECHERS, MCX solid-phase extraction and direct extraction by tert-butyl
                 methyl ether were compared. The results showed that the limit of detection (LOD) of chlorpromazine by
                 three methods was 0.2 μg/kg, and the limit of quantitation (LOQ) was 0.5 μg/kg. At different addition levels,
                 the  recovery  and  relative  standard  deviation  (RSD)  by  three  sample  preparation  methods  were  72.5%~
                 108.8%  and  2.1%~8.6%,  respectively.  All met  the  methodological requirements.  The purifying  effect  of
                 MCX solid-phase extraction was better than the other sample preparation methods. The order of overall
                 recovery was from high to low: MCX solid-phase extraction, QuECHERS, and direct extraction by tert-
                 butyl methyl ether. The recoveries for pork with high fat content by three preparation methods were lower
                 than those of liver, chicken and fish.
                 Key words: chlorpromazine; LC; MS; sample preparation; solid-phase extraction; QuECHERS; tert-butyl
                 methyl ether; feedstuff chemicals


                 氯丙嗪(Chlorpromazine,CPZ),又称冬眠灵、                动物在过量食用药物后会造成体内药物残留                    [4- 5] 。目
            氯普马嗪等,是第一个吩噻嗪类抗精神病药                     [1-2] 。同   前,许多国家和组织已针对各类兽药残留制定了相
            时,氯丙嗪又是一类动物生长促进剂,可以起到使                             应标准   [6-7] 。中国《动物性食品中兽药最高残留限量》
                                                  [3]
            其嗜睡少动、加快生长和改变肉质的作用 。但是,                            (农业部 2002 年第 235 号公告)明确指出:氯丙嗪

                 收稿日期:2019-03-21;  定用日期:2019-05-08; DOI: 10.13550/j.jxhg.20190220
                 基金项目:四川省科技厅应用基础研究计划项目(2016JY0110);农业部农产品质量安全重点实验室开放课题(2018-KF-04);西南科
                 技大学龙山学术人才科研支持计划(17LZX546)
                 作者简介:张   文(1988—),男,博士,讲师,硕士生导师,E-mail:zhangwayne1988@163.com。联系人:黄业传(1975—),男,
                 博士,教授,硕士生导师,E-mail:hyc2005@ sina.com。
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