Page 153 - 精细化工2019年第9期

第 36 卷第 9 期 精 细 化 工 Vol.36, No.9
201 9 年 9 月 FINE CHEMICALS Sept. 2019

医药与日化原料
蓝莓果渣花色苷提取工艺优化及其

提取物的抗肿瘤活性

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薛宏坤 ，谭佳琪 ，刘 钗 ，刘成海
（1. 东北农业大学 工程学院，黑龙江 哈尔滨 150030；2. 东北农业大学 农学院，黑龙江 哈尔滨
150030）
摘要：在单因素实验基础上，通过正交实验优化蓝莓果渣花色苷在浸提（OSE）、微波辅助（MAE）和超声辅助
（UAE）提取 3 种提取方式下的工艺条件，并对比花色苷组分及其提取物抗肿瘤活性。结果表明：微波辅助提
取法获得花色苷含量优于浸提法和超声辅助提取法，花色苷提取条件为：微波功率 300 W、微波时间 60 s、料
液比为 1∶40 g/mL，在此条件下，花色苷含量 46.880.63 mg C3G/g，3 种提取法均获得 6 种花色苷组分，分别
为飞燕草素-3-葡萄糖苷、矢车菊素-3-葡萄糖苷、矢车菊素-3-芸香糖苷、牵牛花素-3-葡萄糖苷、锦葵素-3-半乳
糖苷和芍药素-3，5-二己糖苷。3 种提取方式获得的花色苷提取物对 HepG2 肝癌细胞和 A549 肺癌细胞生长和侵
染均有抑制作用，且对 A549 肺癌细胞抑制效应更强。
关键词：蓝莓果渣；花色苷；工艺；组分；抗肿瘤；医药原料
中图分类号：TS209 文献标识码：A 文章编号：1003-5214 (2019) 09-1881-10

Optimization of Extraction Process of Anthocyanins from
Blueberry Pomace and Its Antitumor Activity

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XUE Hong-kun , TAN Jia-qi , LIU Chai , LIU Cheng-hai
（1. College of Engineering, Northeast Agricultural University, Harbin 150030, Heilongjiang, China; 2. College of
Agricultural, Northeast Agricultural University, Harbin 150030, Heilongjiang, China）
Abstract: Three extraction methods of organic solvent extraction (OSE), microwave-assisted extraction
(MAE) and ultrasonic-assisted extraction (UAE) of anthocyanins from blueberry pomace (BP) were
optimized by single factor test and orthogonal test, and anthocyanins components and their anti-tumor
activity were comparative analyzed. The results showed that MAE was superior to OSE and UAE in
obtaining anthocyanins content. The optimum extracting parameters for anthocyanins from BP were
obtained under microwave power 300 W, extraction time 60 s and solid-to-liquid ratio 1∶40 g/mL. Under
these conditions, anthocyanins contents of 46.88±0.63 mg C3G/g were achieved. The three extraction
methods all obtained six anthocyanins components, which were delphinidin-3-glucoside, cyanidin-3-glucoside,
cyanidin-3-rutinoside, petunidin-3-glucoside, malvidin-3-galactoside and peonidin-3,5-dihexoside. Anthocyanins
obtained by three extraction methods inhibited the growth and invasion ability of HepG2 hepatoma cells
and A549 lung cancer cells, and had stronger inhibitory effect on A549 lung cancer cells.
Key words: blueberry pomace; anthocyanins; process; component; antitumor; drug materials

[2]
目前，花色苷的研究集中在提取、纯化和生理 assisted extraction，MAE） 、超声辅助提取法（Ultrasonic-
[3]
活性等方面。提取方法主要有浸提法（Organic solvent assisted extraction，UAE） 、超高压辅助提取法 [4]
[5]
[1]
extraction，OSE） 、微波辅助提取法（Microwave- 以及酶法辅助提取法 等。结果表明，提取方法不

收稿日期：2019-02-17; 定用日期：2019-04-19; DOI: 10.13550/j.jxhg.20190123
基金项目：东北农业大学“青年才俊”项目（518076）
作者简介：薛宏坤（1988—），男，博士生。联系人：刘成海（1982—），男，副教授，E-mail：liuchenghai@neau.edu.cn。