Page 152 - 《精细化工》2020年第5期

第 37 卷第 5 期 精 细 化 工 Vol.37, No.5
202 0 年 5 月 FINE CHEMICALS May 2020

医药与日化原料
新型功能性植物盐美白功效的体外评价

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严雅丽 ，周沫希 ，姜春鹏 ，赵奕竹 ，张 英
（1. 浙江大学 生物系统工程与食品科学学院 食品科学与营养系，浙江 杭州 310058；2. 上海绿瑞生
物科技有限公司，上海 201203）
摘要：对松盐、梅盐与竹盐的美白功效进行了体外评价，分别研究其对体外酪氨酸酶的单酚酶和二酚酶活性的
抑制作用，并以熊果苷为阳性对照，采用体外培养的 B16 黑素瘤细胞模型探究其对胞内酪氨酸酶活性、细胞增
殖及迁移的影响。结果表明，植物盐元素组成丰富，且水溶液呈碱性，松盐、竹盐、梅盐对酪氨酸酶活性表现
出极显著的浓度依赖的抑制作用（p<0.01），当这 3 种植物盐水溶液的质量浓度为 50.0 g/L 时，它们对酪氨酸酶
单酚酶抑制率分别为 97.1%、94.8%、48.2%，对二酚酶的抑制率均高于 92.0%；与原盐相比，5.0 g/L 的植物盐
对 B16 细胞生长抑制作用不明显，但会抑制其横向迁移；植物盐对 B16 细胞胞内酪氨酸酶活性抑制作用优于熊
果苷，5.0 g/L 的梅盐组的细胞内酪氨酸酶活性仅有 8.76%，抑制效果好于松盐、竹盐。所研究的植物盐可以抑
制酪氨酸酶的活性，且对 B16 细胞无附加毒性，显示出作为一种无机酪氨酸酶抑制剂的潜力，在人体美白方面
有较好的潜在应用价值。
关键词：功能性植物盐；美白作用；酪氨酸酶；B16 细胞；抑制剂；日化原料
中图分类号：TS974.1；TS36 文献标识码：A 文章编号：1003-5214 (2020) 05-1002-08

In vitro evaluation of whitening effects of new functional plant salts

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YAN Yali , ZHOU Moxi , JIANG Chunpeng , ZHAO Yizhu , ZHANG Ying
（1. Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University,
Hangzhou 310058, Zhejiang, China; 2. Shanghai Emerald Biotech Co., Ltd., Shanghai 201203, China）
Abstract: The whitening effects of pine salt, plum salt and bamboo salt were evaluated in vitro, and their
inhibitory effects on the activities of tyrosinase monophenolase and diphenolase were studied in vitro,
respectively. The model of B16 melanoma cells was used to explore the effects of salts on the activity of
intracellular tyrosinase, cell proliferation and migration, and arbutin was used as a positive control. The
results showed that these plant salts were rich in elemental composition and alkaline in aqueous solution.
And pine salt, bamboo salt and plum salt showed extremely significant concentration-dependent inhibition
of tyrosinase activity (p<0.01). When the mass concentration of the three kinds of plant salt solution was
50.0 g/L, their corresponding inhibition rates of tyrosinase monophenolase were 97.1%, 94.8% and 48.2%,
respectively, and those of diphenolase were higher than 92.0%. Compared with the original salt, 5.0 g/L
plant salts had no obvious inhibitory effect on the growth of B16 cells, but they inhibited the lateral
migration. The inhibitory effects of these plant salts on the intracellular tyrosinase activity of B16 cells were
better than that of arbutin, and the intracellular tyrosinase of 5.0 g/L plum salt group was only 8.76%, and
the inhibition effect was better than those of pine salt and bamboo salt. These plant salts can inhibit the
activity of tyrosinase, and have no additional toxicity to B16 cells, showing the potential as an inorganic
tyrosinase inhibitor, and possess very good potential application value in human whitening.
Key words: functional plant salts; whitening effect; tyrosinase; B16 cells; inhibitor; cosmetic materials

收稿日期：2019-11-13; 定用日期：2020-01-17; DOI: 10.13550/j.jxhg.20191075
基金项目：广东省重点研发项目（2019B020212001）
作者简介：严雅丽（1996—），女，硕士生。联系人：张 英（1961—），女，教授，E-mail：yzhang@zju.edu.cn。