Page 165 - 《精细化工》2020年第7期

第 37 卷第 7 期 精 细 化 工 Vol.37, No.7
202 0 年 7 月 FINE CHEMICALS July 2020

医药与日化原料
香根草精油微胶囊的制备及其体外抗炎作用

王一彤 1,2 ，赵新玲 1,2 ，姚佳彤 1,2 ，公衍玲 1,2*

（1. 青岛科技大学 化工学院 药学院，山东 青岛 266042；2. 青岛科技大学 代谢性疾病药物研究重点
实验室，山东 青岛 266042）

摘要：以明胶和壳聚糖为壁层材料，以香根草精油为囊芯，采用复凝聚法制备香根草精油微胶囊。通过单因素
和正交实验筛选微胶囊最佳制备工艺，采用 SEM、粒度仪、Zeta 电位仪和 FTIR 对样品进行了表征。测定微胶
囊于 pH 1.5 和 7.4 时香根草精油的释放率，观察微胶囊对小鼠 RAW 264.7 细胞的体外抗炎作用。结果表明，香
根草精油用量 0.3 g，壁材用量 1.0 g（其中明胶与壳聚糖的质量比为 30∶2）时，封装率可达 90.96%；制备的微
胶囊表面呈球形，微胶囊粒径为 2.303 μm，电位–33.8 mV，具有良好的稳定性，封装效果好；该微胶囊于 pH 7.4
下释放度较佳，72 h 内累积释放达 87.49%；细胞实验表明，该微胶囊（质量浓度为 60、90、120 mg/L）安全无
毒，能有效抑制 RAW 264.7 细胞白介素-1β（IL-1β）、白介素-6（IL-6）和肿瘤坏死因子 α（TNF-α）的分泌。香
根草精油微胶囊处方工艺简单，质量稳定，抗炎活性良好。
关键词：香根草精油；微胶囊；正交实验；RAW 246.7 细胞；炎症因子；医药与日化原料
中图分类号：R945；TQ464 文献标识码：A 文章编号：1003-5214 (2020) 07-1447-07

Preparation of vetiver essential oil microcapsules and
its anti-inflammatory effect in vitro

1,2
1,2
1,2
WANG Yitong , ZHAO Xinling , YAO Jiatong , GONG Yanling 1,2*
（1. Department of Pharmacy, College of Chemical Engineering, Qingdao University of Science and Technology,
Qingdao 266042, Shandong, China; 2. Key Laboratory of Pharmaceutical Research for Metabolic Diseases, Qingdao
University of Science and Technology, Qingdao 266042, Shandong, China）
Abstract: Vetiver essential oil microcapsules were prepared using gelatin and chitosan as the shell material
and vetiver essential oil as the core. The best preparation process of microcapsules was optimaized by
single factor and orthogonal experiments. The samples were characterized by SEM, particle size analyzer,
Zeta potential instrument and FTIR. The release of vetiver essential oil in microcapsules at pH 1.5 and 7.4
was measured, and the anti-inflammatory effect of microcapsules in vitro was studied by mouse RAW
246.7 cells. The results showed that the encapsulation rate reached 90.96% under the conditions of vetiver
essential oil dosage 0.3 g, wall material dosage 1.0 g〔m(gelatin)∶m(chitosan)〕=30∶2. The surface of the
microcapsules was continuous and spherical. The particle size of the microcapsules was 2.303 μm and the
Zeta potential was –33.8 mV. The prepared microcapsules had good stability and excellent packaging effect.
The microcapsules showed a good release character at pH 7.4 with a cumulative release of 87.49% within
72 h. Cell experiments revealed that the microcapsules (60, 90, 120 mg/L) were safe and non-toxic, which
couldd effectively inhibit the interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor alpha
(TNF-α) secretion. The vetiver essential oil microcapsules are simple to prepare and stable in quality with
good anti-inflammatory activity.
Key words: essential oil of vetiver; microcapsules; orthogonal experiment; RAW 246.7 cells; inflammatory
factor; drug and cosmetic materials

收稿日期：2019-12-13; 定用日期：2020-04-09; DOI: 10.13550/j.jxhg.20191168
基金项目：山东省重点研发项目（2018GSF119005）
作者简介：王一彤（1995—），女，硕士生，E-mail：2268526530@qq.com。联系人：公衍玲（1975—），女，副教授，E-mail：
hanyu_ma@126.com。