Page 171 - 《精细化工》2020年第7期

第 7 期 王一彤，等: 香根草精油微胶囊的制备及其体外抗炎作用 ·1453·

实验得到制备香根草精油微胶囊的最佳工艺条件 triple-helix content and mechanical properties of gelatin films[J].
Biomaterials, 2004, 25(25): 5675-5680.
为：明胶与壳聚糖的质量比为 30∶2，香根草精油
[14] GUO R (郭睿), LIU A Y (刘爱玉), GUO Y (郭煜), et al. Process
用量 0.3 g，壁材用量 1.0 g，在此条件下制备出的微 optimization for preparation of carboxymethyl chitosan by response
胶囊封装率可到达到 90.96%。通过扫描电子显微镜 surface methodology[J]. Fine Chemicals (精细化工), 2016, 33(8):
872-879.
的观察，从微观结构上分析微胶囊的表面特征，在 [15] WU Z S (吴宗山), LI L (李莉). Green synthesis of small size silver
最佳制备条件下得到的微胶囊表面呈球形，更符合 nanoparticles by nature product and their antimicrobial properties[J].
Fine Chemicals (精细化工), 2014, 31(8): 964-968.
微胶囊的制备要求。体外释放实验表明，在血液系
[16] LIU J Y, LIU C H, LIU Y J, et al. Study on the grafting of
统（pH 7.4）下 72 h 累积释放达到 87.49%，更适于 chitosan-gelatin microcapsules onto cotton fabrics and its
体内用药。根据细胞实验发现，制备的香根草精油 antibacterial effect[J]. Colloids and Surfaces B-Biointerfaces, 2013,
109(9): 103-108.
微胶囊（质量浓度 60、90、120 mg/L）细胞毒性有 [17] WANG S D (王赛丹), JIANG Z X (江振西), CAO X L (曹秀丽), et
限，用药安全，同时有效地抑制了经 LPS 诱导的 al. Optimization of preparation technology of vetiveroil-β-
cyclodextrin microcapsule[J]. Modern Chemical Industry (现代化
RAW 264.7 细胞炎症因子的产生。香根草精油微胶 工), 2017, 37(1): 142-145, 147.
囊处方工艺简单、质量稳定、抗炎活性良好，为香 [18] ZHOU L G (周利国), WEI F L (魏凤玲). Encapsulation technology
and application of β-cyclodextrin[J]. Medicne Innovation Research
根草精油的开发利用提供了参考。在接下来的研究
(现代保健: 医学创新研究), 2006, 3(3): 31-33.
中，将进一步探讨微胶囊在抗炎药物制备中的应用 [19] ANG L F, DARWIS Y, POR L Y, et al. Microencapsulation
及其抗炎机制。 curcuminoids for effective delivery in pharmaceutical application[J].
Pharmaceutics, 2019, 11(9): 1-22.
[20] CHAO J B, ZHANG B T. Preparation and study on the solid
参考文献：
inclusion complex of cloxacillin sodium with beta-cyclodextrin[J].
[1] LUQMAN S. Investigations on biological activity of Vetiveria Spectrochimica Acta Part A, Molecular and Biomolecular
zizanioides L. Nash, a palingenesis of some important findings in Spectroscopy, 2007, 68(1): 108-112.
miracle grass[J]. Nature Precedings, 2012. DOI: 10.1038/npre.2012.6904. [21] EVAGELIOU V, SALIARI D. Limonene encapsulation in freeze
[2] PAULINE B, ANNE L, MARIE W, et al. Vetiver essential oil in dried gellan systems[J]. Food Chemistry, 2017, 223: 72-75.
cosmetics: What is new?[J]. Medicines, 2017, 4(2): 41-54. [22] KAUSHIK V, ROOS Y H. Limonene encapsulation in freeze-drying
[3] JINDAPUNNAPAT K, REETZ N D, MACDONALD M H, et al. of gum Arabic-sucrose-gelatin systems[J]. LWT-Food Science and
Activity of vetiver extracts and essential oil against meloidogyne Technology, 2007, 40(8): 1381-1391.
incogni ta[J]. Journal of Nematology, 2018, 50(2): 147-162. [23] CHENG C S, ZOU Y, PENG J. Oregano essential oil attenuates Raw
[4] MARTINEZ J, ROSA P T, MENUT C, et al. Valorization of brazilian 264.7 cells from lipopolysaccharide-induced inflammatory response
vetiver (Vetiveria zizanioides (L.) Nash ex small) oil [J]. Journal of through regulating nadph oxidase activation-driven oxidative
Agricultural and Food Chemistry, 2004, 52(21): 6578-6584. stress[J]. Molecules, 2018, 23(8): 1-12.
[5] KRISHNAVENI V, AMPRITHA S. Analysis of phytochemical and [24] KIM M E, JUNG I, LEE J S, et al. Pseudane-Ⅶ isolated from
anti-microbial activity on Vetiveria zizanioides ethanolic extract for Pseudoalteromonas sp. M2 ameliorates LPS-induced inflammatory
healthcare applications[J]. International Journal of Pharma and Bio response in vitro and in vivo[J]. Marine Drugs, 2017, 15(11): 1-12.
Sciences, 2016, 7(2): 758-765. [25] CARMEN R L, BODMEIER R. Effect of formulation and process
[6] WEYERSTAHL P, MARSCHALL H, SPLITTGERBER U, et al. 1, variables on the formation of chitosan-gelatin coacervates[J].
7-Cyclogermacra-1(10), 4-dien-15-al, a sesquiterpene with a novel International Journal of Pharmaceutics, 1996, 135(1/2): 63-72
skeleton, and other sesquiterpenes from Haitian vetiver oil[J]. [26] LI Q, SUN L J, GONG X F, et al. Simultaneous optimization of
Flavour and Fragrance Journal, 2000, 15(2): 61-83. multiple response variables for the gelatin-chitosan microcapsules
[7] FILIiPPI J J, BELHASSEN E, BALDOVINI N, et al. Qualitative and containing angelica essential oil[J]. Iranian Journal of Pharmaceutical
quantitative analysis of vetiver essential oils by comprehensive two-
Research, 2017, 16(1): 50-62.
dimensional gas chromatography and comprehensive two-dimensional [27] YUEN C W M, YIP J, LIU L, et al. Chitosan microcapsules loaded
gas chromatography/mass spectrometry[J]. Journal of Chromatography
A, 2013, 1288: 127-148. with either miconazole nitrate or clotrimazole, prepared via emulsion
[8] LUQMAN S, KUMAR R, KAUSHIK S, et al. Antioxidant potential technique[J]. Carbohydrate Polymers, 2012, 89: 795-801.
of the root of Vetiveria zizanioides (L.) Nash[J]. Indian Journal of [28] NATRAJAN D, SRINIVASAN S, SUNDAR K, et al. Formulation
Biochemistry & Biophysics, 2009, 46(1): 122-125. of essential oil-loaded chitosan-alginate nanocapsules[J]. Journal of
[9] LUQMAN S, SSIVASTAVA S, DAROKAR M P, et al. Detection of Food and Drug Analysis, 2015, 23(3): 560-568.
antibacterial activity in spent roots of two genotypes of aromatic [29] HUSSAIN M R, MAJI T K. Preparation of genipin cross-linked
grass Vetiveria zizanioides[J]. Pharmaceutical Biology, 2008, 43(8): chitosan-gelatin microcapsules for encapsulation of Zanthoxylum
732-736. limonella oil (ZLO) using salting-out method[J]. J Microencapsul,
[10] SAIKIA D, PARVEEN S, GUPTA V K, et al. Anti-tuberculosis 2008, 25(6): 414-420.
activity of indian grass khus (Vetiveria zizanioides L. Nash)[J]. [30] STAROSZCZYk H, SZTUKA K, WOLSKA J, et al. Interactions
Complementary Therapies in Medicine, 2012, 20(6): 434-436. of fish gelatin and chitosan in uncrosslinked and crosslinked with
[11] MA G. Microencapsulation of protein drugs for drug delivery: EDC films: FTIR study[J]. Spectrochimica Acta Part A, Molecular
Strategy, preparation, and applications[J]. Journal of Controlled and Biomolecular Spectroscopy, 2014, 117: 707-712.
Release : Official Journal of the Controlled Release Society, 2014, [31] OISHI Y, MANABE I. Macrophages in inflammation, repair and
193, 324-340. regeneration[J]. International Immunology, 2018, 30(11): 511-528.
[12] SBASTIEN G. Microencapsulation: industrial appraisal of existing [32] GUO L M (郭立敏), LV J L (吕洁丽), ZHANG L B (张来宾).
technologies and trends[J]. Trends in Food Science & Technology, Pesearch progress on anti-inflammatory mechanism of natural
2004, 15(7/8): 330-347. sesquiterpenoids[J]. China Journal of Chinese Materia Medica (中国
[13] BIGI A, PANZAVOLTA S, RUBINI K. Relationship between 中药杂志), 2018, 43(20): 3989-3999.