Page 134 - 《精细化工》2021年第10期

第 38 卷第 10 期 精 细 化 工 Vol.38, No.10
2021 年 10 月 FINE CHEMICALS Oct. 2021

中药现代化技术
冬凌草硒多糖的制备及其抗氧化活性分析

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程 爽 ，贺 斐 ，付龙洋 ，章亚东 1,2*
（1. 郑州大学 化工学院，河南 郑州 450001；2. 郑州大学 济源研究院，河南 济源 459000）

摘要：以济源冬凌草为原料，水提得到冬凌草多糖，对多糖进行结构修饰制得冬凌草硒多糖。采用 UV、FTIR、
SEM、TGA、HPLC 对冬凌草多糖以及硒多糖结构进行表征；通过 1,1-二苯基-2-三硝基苯肼（DPPH）自由基法、
2,2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）自由基法、羟基自由基法以及还原能力测定 4 种方法
对冬凌草多糖及其硒多糖的抗氧化活性进行探究。结果表明，冬凌草硒多糖中硒含量为 1.35 mg/g。硒化修饰后，
冬凌草多糖的基本骨架得到保留，单糖种类未发生改变，但分解温度降低、稳定性下降，多糖形貌也发生明显
变化，球状与条状形貌增多，片状形貌减少。在体外抗氧化性实验中，当冬凌草硒多糖质量浓度为 1.6 g/L 时，
对 DPPH 自由基、ABTS 自由基、羟基自由基清除率分别为 68.69%、86.90%、45.12%，均强于冬凌草多糖。
关键词：冬凌草；多糖；硒；表征；抗氧化活性；中药现代化技术
中图分类号：O629.12；O613.52；R284 文献标识码：A 文章编号：1003-5214 (2021) 10-2064-08

Preparation of selenium polysaccharide from Rabdosia rubescens
and analysis of its antioxidant activity

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CHENG Shuang , HE Fei , FU Longyang , ZHANG Yadong 1,2*
（1. School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, Henan, China; 2. Jiyuan Research
Institute, Zhengzhou University, Jiyuan 459000, Henan, China）

Abstract: Polysaccharides were obtained by hot water extraction from Jiyuan Rabdosia rubescens and then
selenium polysaccharides were prepared. The structures of polysaccharides from Rabdosia rubescens and
selenium polysaccharides were analyzed by UV, FTIR, SEM, TGA and HPLC. The antioxidant activities of
polysaccharides from Rabdosia rubescens and selenium polysaccharides were evaluated by 1,1-diphenyl-2-
trinitrophenylhydrazine (DPPH) free radical assay, 2,2-diazide-bis (3-ethyl-benzothiazole-6-sulfonic acid)
diammonium salt (ABTS) free radical assay, hydroxyl free radical assay and reduction ability assay. The
results showed that the selenium content in selenium polysaccharides was 1.35 mg/g. After selenization, the
basic skeleton of polysaccharides from Rabdosia rubescens was retained, and monosaccharide species did
not change, but the stability and decomposition temperature of polysaccharides decreased. The morphology
of polysaccharides also changed significantly, with more spherical and strip morphologies and less flake
morphologies. In vitro antioxidation experiments indicated that the scavenging rates of DPPH free radical,
ABTS free radical and hydroxyl free radical by selenium polysaccharides with a mass concentration of 1.6
g/L were 68.69%, 86.90% and 45.12%, respectively, which were all better than those of polysaccharides
from Rabdosia rubescens.
Key words: Rabdosia rubescens; polysaccharides; selenium; characterization; antioxidant activities;
modernization technology of traditional Chinese medicines

冬凌草〔Rabdosia rubescens (Hemsl.) Hara〕也 燥地上部分。研究表明，冬凌草具有清热解毒、减
被称为冰凌草，为唇形科香茶菜属植物碎米桠的干 少炎症、促进血液循环、消肿、抗肿瘤等功效。冬

收稿日期：2021-03-25; 定用日期：2021-06-23; DOI: 10.13550/j.jxhg.20210320
基金项目：河南省财政厅 2018 年中央引导地方科技发展专项（2060599）
作者简介：程 爽（1995—），女，硕士生，E-mail：2531278010@qq.com。联系人：章亚东（1965—），男，教授，博士，E-mail：
Zhangyadong2016@163.com。