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·2070·                            精细化工   FINE CHEMICALS                                 第 38 卷

                 关于冬凌草多糖抗氧化活性的报道很少,但使                          [6]   HU H B, LIANG H P, LI H M,  et al. Isolation,  purification,
                                                                   characterization and antioxidant activity of polysaccharides from the
            用其他材料进行的类似报道较多。商龙臣等                     [36] 研究        stem barks of Acanthopanax leucorrhizus[J]. Carbohydrate Polymers,
            了南瓜硒多糖的活性,分析发现,南瓜硒多糖对超                                 2018, 196: 359-367.
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            氧阴离子自由基、OH 自由基的清除作用显著强于                                on preparation and antioxidant activity of the seleno-polysaccharides
            南瓜多糖,与样品量呈正相关;侯巍等                   [37] 对玉米须          from Tussilago farfara[J]. Science and Technology of Food Industry
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            硒多糖的抗氧化活性进行研究发现,玉米须硒多糖                             [8]   WANG J, ZHAO B, WANG X, et al. Synthesis of selenium-containing
            对 OH 自由基的清除能力稍强于玉米须多糖,对超                               polysaccharides and evaluation of antioxidant activity  in vitro[J].
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            氧阴离子自由基的清除能力明显强于玉米须多糖,                                 987-991.
            当玉米须硒多糖的质量浓度为 1.0 g/L 时,对 DPPH                     [9]   ZHANG Z H (张志红), LIU C (刘臣). Determination of selenium in
                                                                   the enriched rice  with  O-phenylenediamine UV spectrophotometry
            自由基的清除率为 94.9%,与抗坏血酸相近;REN                             [J]. Journal of Beijing Institute of Petro-chemical Technology (北京
            等 [38] 发现,香菇多糖及硒多糖的还原能力随其浓度                            石油化工学院学报), 2015, 23(3): 11-14.
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            的增加而逐渐增加,且硒多糖还原能力明显高于多                                 polysaccharides of grifola frondosa fruit body[D]. Zhenjiang: Jiangsu
            糖,与本实验结果较为一致。但是其他多糖(青蒿                                 University (江苏大学), 2014.
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            种子多糖     [39] 、铁线莲多糖   [40] 、西兰花多糖    [41] )与冬         pyrazolone-derivatized neutral, acidic and basic aldoses[J]. Journal of
            凌草多糖、其他硒多糖(香菇多糖                [28] 、枸杞多糖  [24] 、      Chromatography A, 1994, 678(1): 17-23.
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            牡蛎多糖     [42] )与冬凌草硒多糖之间抗氧化活性能力                        of polysaccharides from dunaliella salina by modified  RP-HPLC
            存在较大差异,原因是不同植物的多糖类型不同,                                 method of precolumn derivatization with 1-phenyl-3-methyl-5-
                                                                   pyrazolone[J]. Carbohydrate Polymers, 2010, 82(3): 629-635.
            具有不同的理化性质和结构特征,因此抗氧化活性                             [13]  ZHANG J J, ZHANG Q  B, WANG J,  et al. Analysis of the
            也有一定差异。                                                monosaccharide composition of fucoidan by precolumn  derivation
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            3   结论                                             [14]  CHEN J M (陈晋明), FENG C P (冯翠萍). Antioxidation activty of
                                                                   pleurotus nebrodensis polysaccharide[J]. Food Science and Technology
                                                                   (食品科技), 2015, 40(2): 239-242.
                 以冬凌草多糖为原料,Na 2 SeO 3 为硒化试剂,进                  [15]  ZHANG D Y, LUO M, WANG W, et al. Variation of active constituents
            行冬凌草硒多糖的合成。硒多糖在 334 nm 处有明                             and antioxidant activity in pyrola [P. incarnata Fisch.] from different
                                                                   sites in northeast China[J]. Food Chemistry, 2013, 141(3): 2213-2219.
            显紫外吸收峰;红外光谱分析发现,硒多糖在 1049、                         [16]  LI S, SHAH N P. Characterization, antioxidative and bifidogenic
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            880 cm 处出现了新的吸收峰,证明产物结构中含                              effects of polysaccharides from Pleurotus eryngii after heat treatments[J].
                                                                   Food Chemistry, 2016, 197(A): 240-249.
            有 O—Se—O、Se==O 键,均表明冬凌草多糖已被成                       [17]  SONG J M (宋佳敏), WANG H F (王鸿飞), SUN M (孙朦), et al.
            功硒化修饰。热重分析发现,亚硒酸根的引入使得                                 Optimization  of extraction and antioxidant activity  of  polysaccharides
                                                                   from cordyceps  cicadae by response surface  methodology[J]. Food
            多糖稳定性下降,分解温度降低。体外抗氧化实验                                 Science (食品科学), 2018, 39(4): 275-281.
            表明,冬凌草硒多糖对 DPPH 自由基、ABTS 自由                        [18] ZHU L (朱良), ZHANG J P (张杰平), WANG Y F (王一飞). Study
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            基和 OH 自由基的清除能力及还原能力均强于冬凌                               Science (食品科学), 2008, 29(3): 453-456.
            草多糖。以上结果为冬凌草硒多糖的研究提供了一                             [19]  WEI D, CHEN T, YAN M, et al. Synthesis, characterization, antioxidant
                                                                   activity and neuroprotective effects of selenium polysaccharide from
            定的基础,并为其作为新型保健功能的补硒试剂提                                 Radix hedysari[J]. Carbohydrate Polymers, 2015, 125: 161-168.
            供了一定的技术参考。                                         [20]  WANG Z Y (王占一), LI Z W (李卓瓦), BI H D (毕海丹),  et al.
                                                                   Optimization of synthesis technology of  selenium polysaccharide
                                                                   ester from pomegranate seeds and its anti-lipid oxidation ability[J].
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