Page 141 - 《精细化工》2021年第7期

第 38 卷第 7 期 精 细 化 工 Vol.38, No.7
202 1 年 7 月 FINE CHEMICALS July 2021

催化与分离提纯技术
响应面优化马桑皮没食子酸的酶法提取技术

3*
1*
1
郭林新 1,2 ，强涛涛 ，马养民 ，任龙芳
（1. 陕西科技大学 轻工科学与工程学院，陕西 西安 710021；2. 安康学院 化学化工学院，陕西 安康
725000；3. 中国轻工业轻化工助剂重点实验室，陕西 西安 710021）

摘要：采用单宁酶法从马桑皮中提取没食子酸，以单因素-响应面实验优化了提取工艺，对得到的没食子酸进行
了纯化和结构鉴定，考察了纯化后没食子酸的抗氧化活性，并与传统的酸提取法相对比。结果表明，在马桑皮
质量 2.00 g、固液比 1∶40（g∶mL）、单宁酶添加量为 20 U/g（以马桑皮的质量为基准）、体系 pH 5.0、提取温
度 48 ℃、提取时间 4 h 的条件下，没食子酸提取率可达 10.79%±0.65%。经 NKA-9 大孔吸附树脂纯化后，酶法
制备的没食子酸的纯度为 96.0%，高于酸提取法制备的没食子酸纯度（81.5%）。与酸提取法相比，酶法制备的
没食子酸具有更强的抗氧化活性，在质量浓度为 256 mg/L 时，其对 1,1-二苯基-2-三硝基苯肼自由基（DPPH•）
3+
和羟基自由基（•OH）的清除率分别为 78.81%和 61.99%，对 Fe 还原能力为 0.427。
关键词：响应面；单宁酶；马桑皮；没食子酸；提取技术
中图分类号：TQ28 文献标识码：A 文章编号：1003-5214 (2021) 07-1423-07

Enzymatic extraction of gallic acid from Coriaria nepalensis bark
by response surface optimization

1
3*
1,2
1*
GUO Linxin , QIANG Taotao , MA Yangmin , REN Longfang
（1. College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science & Technology, Xi'an
710021, Shaanxi, China; 2. College of Chemistry and Chemical Engineering, Ankang University, Ankang 725000, Shaanxi,
China; 3. Key Laboratory of Chemical Additives for China National Light Industry, Xi'an 710021, Shaanxi, China）
Abstract: Tannase was used to extract gallic acid from Coriaria nepalensis bark, and the extraction process
was optimized by single factor and response surface experiments. The obtained gallic acid was purified and
identified. The antioxidant activity of the purified gallic acid was investigated and compared with that of the
sample extracted by traditional acid extraction method. The results showed that when Coriaria nepalensis
bark mass was 2.00 g, solid liquid ratio was 1∶40 (g∶mL), tannase addition amount was 20 U/g (based on
the mass of Coriaria nepalensis bark), pH of the system was 5.0, extraction temperature and extraction time
was 48 ℃ and 4 h, the extraction yield of gallic acid could reach 10.79%±0.65%. After purification by
NKA-9 macroporous adsorption resin, the purity of gallic acid prepared by enzymatic method was 96.0%,
which was higher than that of gallic acid (81.5%) prepared by acid extraction method. Compared with that
prepared by acid extraction method, gallic acid prepared by enzymatic method had stronger antioxidant activity.
The scavenging rate of 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH•) and hydroxyl radicals (•OH) by
gallic acid with a mass concentration of 256 mg/L were 78.81% and 61.99%, respectively, and the reduction
3+
ability of Fe was 0.427.
Key words: response surface; tannase; Coriaria nepalensis bark; gallic acid; extraction technology

没食子酸（GA）是一种天然的多酚类物质，常 重要的精细化学品，没食子酸化学性质活泼并具有
[3]
[4]
[2]
[1]
存在于五倍子 、石榴 、葡萄等植物中 。作为一种 多样的生物活性，广泛地用于医药 、食品 [5-6] 、化工

收稿日期：2020-12-30; 定用日期：2021-03-11; DOI: 10.13550/j.jxhg.20201256
基金项目：陕西省重点科技创新团队（2020TD-009）；陕西省教育厅重点科学研究计划（协同创新中心项目）（20JY003）
作者简介：郭林新（1990—），男，博士生，E-mail：glx9933@foxmail.com。联系人：强涛涛（1980—），男，教授，E-mail：qiangtt515@163.com；
马养民（1963—），男，教授，E-mail：mym63@sina.com。