Page 154 - 《精细化工》2022年第11期

·2304· 精细化工 FINE CHEMICALS 第 39 卷

3 结论 [12] SONG Y R, HAN A R, PARK S G, et al. Effect of enzyme-assisted
extraction on the physicochemical properties and bioactive potential
of lotus leaf polysaccharides[J]. International Journal of Biological
（1）采用纤维素酶辅助和微波辅助提取法提取 Macromolecules, 2020, 153: 169-179.
[13] DUBOIS M, GILLES K A, HAMILTON J K, et al. Colorimetric
的琉璃苣叶多糖 BLP-1 和 BLP-2 理化性质不同，其
method for determination of sugars and related substances[J].
对应的相对分子质量分别为 20103 和 22652。BLP-1 Analytical Chemistry, 1956, 28(3): 350-356.
[14] BRADFORD M M. A rapid and sensitive method for the quantitation
和 BLP-2 主要由 Ara、Gal、Glc、Xyl 组成，但其摩 of microgram quantities of protein utilizing the principle of
尔分数不同；其最小降解温度分别为 247 和 269 ℃ protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1/2): 248-
254.
（2）BLP-2 对 α-葡萄糖苷酶和 α-淀粉酶的抑制 [15] LIU X, ZHU L C, TAN J, et al. Glucosidase inhibitory activity and
作用强 于 BLP-1 ；低质 量浓度 的 BLP-2 （ 300~ antioxidant activity of flavonoid compound and triterpenoid
compound from Agrimonia pilosa Ledeb[J]. BMC Complementary
900 mg/L）对 A549、MX-1 和 HepG-2 细胞的抑制 and Alternative Medicine, 2014, 14(1): 1-10.
率高于 BLP-1；BLP-1 和 BLP-2 可通过增强免疫细 [16] AKHTAR H M S, ABDIN M, HAMED Y S, et al. Physicochemical,
functional, structural, thermal characterization and α-amylase inhibition
胞吞噬大分子、分泌 NO 及 IL-6 细胞因子的能力来 of polysaccharides from chickpea (Cicer arietinum L.) hulls[J].
刺激免疫调节活性。 LWT-Food Science and Technology, 2019, 113: 108265.
[17] FELICE D L, SUN J, LIU R H. A modified methylene blue assay for
（3）有关 BLP-1 和 BLP-2 结构表征、BLP 的体 accurate cell counting[J]. Journal of Functional Foods, 2009, 1(1):
109-118.
内降糖活性和抗癌机制有待进一步研究，开发天然 [18] SHI Z J, XIAO L P, DENG J, et al. Isolation and characterization of
低价的降血糖药物，有利于琉璃苣叶资源的综合开 soluble polysaccharides of Dendrocalamus brandisii[J]. BioResources,
2011, 6(4): 5151-5166.
发利用。 [19] GIAVASIS I. Bioactive fungal polysaccharides as potential functional
两种多糖可作为新功能食品和医药产品中的生 ingredients in food and nutraceuticals[J]. Current Opinion in
Biotechnology, 2014, 26: 162-173.
物活性成分，为进一步研究两种多糖的构效关系提 [20] TANG S M. Astragalus polysaccharide improves type 2 diabetes
供了理论依据。 mellitus in rats by protecting islet β cells[J]. Academic Journal of
Second Military Medical University, 2017, 38(4): 482-487.
[21] LIU Y T, CHEN D, YOU Y X, et al. Structural characterization and
参考文献： antidiabetic activity of a glucopyranose-rich heteropolysaccharide
[1] REN F (任飞), HAN F (韩发), SHI L N (石丽娜), et al. Supercritical from Catathelasma ventricosum[J]. Carbohydrate Polymers, 2016,
CO 2 fluid extraction of borage seed oil and its fatty acid composition 149: 399-407.
analysis[J]. China Oils and Fats (中国油脂), 2010, 35(8): 15-18. [22] CHENG S (程爽), HE F (贺斐), ZHANG Y D (章亚东), et al.
[2] LI T (李童). Study on the extraction process and the antioxidant Preparation of selenium polysaccharide from Rabdosia rubescens
activity of borage seed oil and its product development[D]. and analysis of its antioxidant activity[J]. Fine Chemicals (精细化
Yangzhou: Yangzhou University (扬州大学), 2017. 工), 2021, 38(10): 2064-2071.
[3] ZHU K X, ZHANG Y J, NIE S P, et al. Physicochemical properties [23] XU H Y, LIU L Y, MIAO D, et al. Effect of Ganoderma applanatum
polysaccharides on MAPK/ERK pathway affecting autophagy in
and in vitro antioxidant activities of polysaccharide from Artocarpus breast cancer MCF-7 cells[J]. International Journal of Biological
heterophyllus Lam. pulp[J]. Carbohydrate Polymers, 2017, 155: 354- Macromolecules, 2020, 146: 353-362.
361.
[4] HU J L, NIE S P, XIE M Y. Antidiabetic mechanism of dietary [24] ZHANG Y, ZHANG Y N, GAO W H, et al. A novel antitumor
protein from the mushroom Pholiota nameko induces apoptosis of
polysaccharides based on their gastrointestinal functions[J]. Journal human breast adenocarcinoma MCF-7 cells in vivo and modulates
of Agricultural and Food Chemistry, 2018, 66(19): 4781-4786. cytokine secretion in mice bearing MCF-7 xenografts[J]. International
[5] LI C, LI X S YOU L J, et al. Fractionation, preliminary structural Journal of Biological Macromolecules, 2020, 164: 3171-3178.
characterization and bioactivities of polysaccharides from Sargassum [25] MA Q, JIANG J G, YUAN X H, et al. Comparative antitumor and
pallidum[J]. Carbohydrate Polymers, 2017, 155: 261-270. anti-inflammatory effects of flavonoids, saponins, polysaccharides,
[6] LIU Y L, YIN R Q, LIANG S S, et al. Effect of dietary Lycium essential oil, coumarin and alkaloids from Cirsium japonicum DC[J].
barbarum polysaccharide on growth performance and immune Food and Chemical Toxicology, 2019, 125: 422-429.
function of broilers[J]. Journal of Applied Poultry Research, 2017, [26] KHAN T, DATE A, CHAWDA H, et al. Polysaccharides as potential
26(2): 200-208. anticancer agents—A review of their progress[J]. Carbohydrate
[7] CHEN B C, MCCLEMENTS D J, DECKER E A. Role of continuous Polymers, 2019, 210: 412-428.
phase anionic polysaccharides on the oxidative stability of menhaden [27] SCHEPETKIN I A, FAULKNER C L, NELSON-OVERTON L K,
oil-in-water emulsions[J]. Journal of Agricultural and Food Chemistry, et al. Macrophage immunomodulatory activity of polysaccharides
2010, 58(6): 3779-3784. isolated from Juniperus scopolorum[J]. International
[8] CHEN L, GE M D, ZHU Y J, et al. Structure, bioactivity and Immunopharmacology, 2005, 5(13/14): 1783-1799.
applications of natural hyperbranched polysaccharides[J]. Carbohydrate [28] CHEN Y H, WAN X Z, WU D S, et al. Characterization of the
Polymers, 2019, 223: 115076. structure and analysis of the anti-oxidant effect of microalga
[9] WU G H, HU T, HUANG Z L, et al. Characterization of water and Spirulina platensis polysaccharide on Caenorhabditis elegans mediated
alkali-soluble polysaccharides from Pleurotus tuber-regium sclerotia[J]. by modulating microRNAs and gut microbiota[J]. International
Carbohydrate Polymers, 2013, 96(1): 284-290. Journal of Biological Macromolecules, 2020, 163: 2295-2305.
[10] SOVOVÁ H, STATEVA R P, GALUSHKO A A. Solubility of [29] LIU W J, LI W Y, SUI Y, et al. Structure characterization and anti-
β-carotene in supercritical CO 2 and the effect of entrainers[J]. The leukemia activity of a novel polysaccharide from Angelica sinensis
Journal of Supercritical Fluids, 2001, 21(3): 195-203. (Oliv.) diels[J]. International Journal of Biological Macromolecules,
[11] HU Z Y, ZHOU H L, ZHAO J L, et al. Microwave-assisted extraction, 2019, 121: 161-172.
characterization and immunomodulatory activity on RAW264.7 cells [30] USOLTSEVA R V, ANASTYUK S D, SHEVCHENKO N M, et al.
of polysaccharides from Trichosanthes kirilowii Maxim seeds[J]. Polysaccharides from brown algae Sargassum duplicatum: The
International Journal of Biological Macromolecules, 2020, 164: structure and anticancer activity in vitro[J]. Carbohydrate Polymers,
2861-2872. 2017, 175: 547-556.