Page 126 - 《精细化工》2022年第2期

第 39 卷第 2 期 精 细 化 工 Vol.39, No.2
2022 年 2 月 FINE CHEMICALS Feb. 2022

中药现代化技术
红脚艾和汤阴北艾精油的化学成分及抗菌活性

李 杰 1,2 ，朱丹霞 1,2 ，余 平 1,2 ，黄建香 1,2 ，林泽斌 1,2*

（1. 广东暨晴生物医药科技有限公司，广东 惠州 516081；2. 国家中药现代化工程技术研究中心 康养
资源分中心，广东 惠州 516081）

摘要：通过水蒸气蒸馏（SD）法和超临界流体萃取-分子蒸馏（SFE-MD）法从红脚艾和汤阴北艾中提取精油，
采用 GC-MS 检测精油化学成分，并测定其抗菌活性。结果表明，SD 法提取精油收率比 SFE-MD 法高 1.2~2.7
倍。红脚艾精油主成分为 β-石竹烯、龙脑、氧化石竹烯和大根香叶烯 D 等；北艾精油主成分为桉油精、松油
烯-4-醇和十氢二甲基甲乙烯基萘酚等；北艾精油以单萜类化合物为主，而红脚艾精油则含有更丰富的倍半萜类
化合物；SFE-MD 会导致部分单萜类化合物的损失，如桉油精。艾叶精油对大肠杆菌、金黄色葡萄球菌、白色
念珠菌有抑制及杀灭效果，且抗菌效果北艾精油优于红脚艾精油。
关键词：艾叶精油；水蒸气蒸馏；超临界萃取；化学成分；抗菌活性；中药现代化技术
中图分类号：TQ654+.2 文献标识码：A 文章编号：1003-5214 (2022) 02-0330-07

Chemical components and antibacterial activities of essential oils of

Artemisia Verlotorum and Artemisia Argyi from Tangyin county

1,2
1,2
1,2
1,2
LI Jie , ZHU Danxia , YU Ping , HUANG Jianxiang , LIN Zebin 1,2*
（1. Guangdong Geeching Biomedical Technology Company Ltd., Huizhou 516081, Guangdong, China; 2. Healthcare
Resource Branch, National Engineering Research Center for Modernization of Traditional Chinese Medicine, Huizhou
516081, Guangdong, China）
Abstract: Artemisia verlotorum and Artemisia argyi from Tangyin county were extracted by steam
distillation (SD) method and supercritical fluid extraction combined molecular distillation (SFE-MD)
method, and then the chemical components and antibacterial activities of the obtained essential oils were
analyzed and determined by GC-MS. The results showed that the yield of essential oil extracted by SD
method was 1.2~2.7 times higher than that by SFE-MD method. The main components of essential oil of
Artemisia verlotorum were β-caryophyllene, endo-borneol, caryophyllene oxide and germacrene D, etc.
While thoses of essential oil of Artemisia argyi were eucalyptol, terpinene-4-ol and neointermedeol, etc.
The essential oil of Artemisia argyi was mainly composed of monoterpenoids, while that of Artemisia
verlotorum contained more sesquiterpenoids. SFE- MD could resulted in the loss of some monoterpenoids,
such as eucalyptol. The essential oil of wormwood leaf had an excellent bacteriostatic and bactericidal
effects on Escherichia coli, Staphylococcus aureus and Candida albicans. The antibacterial effect of
essential oil of Artemisia argyi was better than that of essential oil of Artemisia verlotorum.
Key words: essential oil of wormwood leaf; steam distillation; supercritical fluid extraction; chemical
components; antibacterial activity; modernization technology of traditional Chinese medicine

[2]
艾叶为菊科蒿属植物艾（Artemisia argyi Lévl. 材，但在宋代，汤阴北艾曾为艾叶道地药材 。近
et Vant.）的干燥叶，具有温经止血、散寒止痛等功 年来，汤阴北艾成为汤阴政府重点支持发展的绿色
[1]
效 。目前，湖北蕲春的蕲艾被认为是艾叶道地药 健康产业，种植面积不断扩大，但关于汤阴北艾尤

收稿日期：2021-09-09; 定用日期：2021-10-20; DOI: 10.13550/j.jxhg.20210911
作者简介：李 杰（1987—），男，硕士，E-mail：lij@kxbtd.com。联系人：林泽斌（1990—），男，硕士，E-mail：linzb@kxbtd.com。