Page 221 - 《精细化工》2022年第3期

第 3 期 张弘弛，等: FgaPT2 酶催化合成 C-4 异戊烯基化吲哚二酮哌嗪和定向诱变增强收率 ·643·

瘤、抗细菌、抗真菌和抗氧化活性。其中，环-L-4- an Aspergillus terreus prenyltransferase[J]. Applied Microbiology
and Biotechnology, 2015, 99: 1719-1730.
二甲基烯丙基-色氨酸-L-色氨酸表现出最高的综合 [16] LIU R, ZHANG H C, WU W Q, et al. C7-prenylation of tryptophan-
生物学活性。基于分子模型和关键残基，确定 containing cyclic dipeptides by 7-dimethylallyl tryptophan synthase
significantly increases the anticancer and antimicrobial activities[J].
Arg-244 为 FgaPT2 的异戊烯基化活性的饱和诱变位 Molecules, 2020, 25: 3676.
点。诱变结果显示，52.6%的 FgaPT2_R244X 突变体 [17] ZHOU K, ZHAO W, LIU X Q, et al. Saturation mutagenesis on
Tyr205 of the cyclic dipeptide C2-prenyltransferase FtmPT1 results
可以增强对环-L-色氨酸-L-色氨酸的催化能力，而 in mutants with strongly increased C3-prenylating activity[J]. Applied
21.1%的突变体具有与 FgaPT2 相似的催化能力。环- Microbiology and Biotechnology, 2016, 100: 9943-9953.
[18] FAN A L, ZOCHER G, STEC E, et al. Site-directed mutagenesis
L-色氨酸-L-色氨酸和突变的氨基酸之间的动力学 switching a dimethylallyl tryptophan synthase to a specific tyrosine
参数分析也支持这一结论。这意味着所获得的具有 C3-prenylating enzyme[J]. Journal of Biological Chemistry, 2015,
290: 1364-1373.
较高催化效率的突变体可以作为生产 C-4 异戊烯基 [19] FAN A L, LI S M. Saturation mutagenesis on Arg-244 of the
化吲哚二酮哌嗪的有效催化剂。 tryptophan C4-prenyltransferase FgaPT2 leads to enhanced catalytic
ability and different preferences for tryptophan-containing cyclic
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