Page 116 - 《精细化工》2022年第4期

第 39 卷第 4 期 精 细 化 工 Vol.39, No.4
2022 年 4 月 FINE CHEMICALS Apr. 2022

医药与日化原料
乳酸菌发酵提取物保护皮肤氧化损伤的作用与机理

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赵 丹，刘平平，李 萌 ，王昌涛，张佳婵，王冬冬
（北京工商大学 化学与材料工程学院 北京市植物资源研究开发重点实验室，北京 100048）

摘要：通过发酵培养获得了 5 种乳酸菌发酵提取物：植物乳杆菌发酵提取物（FE1）、类干酪乳杆菌发酵提取物
（FE2）、嗜酸乳杆菌发酵提取物（FE3）、高加索酸奶乳杆菌发酵提取物（FE4）、瑞士乳杆菌发酵提取物（FE5）。
从生化、细胞、分子 3 种水平对所得提取物的抗氧化功效和保护细胞氧化应激损伤的效果和机制进行检测。质
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量浓度为 10 g/L FE1 清除自由基的能力最强，可清除 49.14%的•OH 和 73.99%的•O 2 。质量浓度为 10 g/L 的 FE1、
FE2 和 FE3 能够显著提高氧化应激损伤条件下人皮肤成纤维细胞的存活率与谷胱甘肽过氧化物酶活性，降低细
胞内活性氧含量。FE1 保护下胞内过氧化氢酶、超氧化物歧化酶活性及总抗氧化能力显著提升。FE1 通过促进
Sirt1 以及 Wnt/β-catenin 通路中 β-catenin、SMP30、FoxO3a、Tbx3 的表达以提升抗氧化能力。结果表明，FE1
良好的抗氧化功效能够减缓 H 2 O 2 刺激引起皮肤细胞的氧化损伤。
关键词：乳酸菌；发酵提取物；保护；氧化；损伤；医药与日化原料
中图分类号：TQ658；TQ920.1 文献标识码：A 文章编号：1003-5214 (2022) 04-0752-09

Effect and mechanism of lactic acid bacteria fermentation
extracts to protect skin from oxidative stress damage

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ZHAO Dan, LIU Pingping, LI Meng , WANG Changtao,
ZHANG Jiachan, WANG Dongdong
(Beijing Key Laboratory of Plant Resources Research and Development, College of Chemistry and Materials
Engineering, Beijing Technology and Business University, Beijing 100048, China)

Abstract: Five kinds of lactic acid bacteria fermentation extracts, Lactobacillus plantarum fermentation
extract (FE1), Lactobacillus paracasei fermentation extract (FE2), Lactobacillus acidophilus fermentation
extract (FE3), Lactobacillus kefiri fermentation extract (FE4) and Lactobacillus helveticus fermentation
extract (FE5) were obtained by fermentation culture. The antioxidant effect and mechanism of protecting
cells from oxidative stress damage of the obtained extracts were tested at biochemical, cellular, and
molecular levels. Mass concentration of 10 g/L FE1 had the strongest ability to scavenge free radicals,
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which could scavenge 49.14% of •OH and 73.99% of •O 2 . Mass concentration of 10 g/L FE1, FE2 and FE3
could significantly improve the survival rate and glutathione peroxidase activity of human skin fibroblasts
and reduce the content of reactive oxygen species in cells under oxidative stress damage conditions. Under
the protection of FE1, the activities of intracellular catalase, superoxide dismutase and total antioxidant
capacity were significantly increased. FE1 enhanced the antioxidant capacity by upregulating the expression
of Sirt1 and genes in Wnt/β-catenin pathways including β-catenin, SMP30, FoxO3a, and Tbx3. The above
results indicated that the good antioxidant effect of FE1 could slow down the oxidative stress damage of
skin cells caused by H 2O 2.
Key words: lactic acid bacteria; fermentation extract; protection; oxidaton; damage; drug and cosmetic
materials

收稿日期：2021-09-08; 定用日期：2021-12-08; DOI: 10.13550/j.jxhg.20210907
基金项目：国家自然科学基金（31971382）
作者简介：赵 丹（1988—），女，实验师，E-mail：zhaodanustb@126.com。联系人：李 萌（1981—），女，副教授，E-mail：
mengmengli@163.com。