Page 96 - 《精细化工》2022年第5期

第 39 卷第 5 期 精 细 化 工 Vol.39, No.5
2022 年 5 月 FINE CHEMICALS May 2022

医药与日化原料
硒化二氢杨梅素的制备及对肿瘤细胞

增殖/转移的抑制性

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毛 敏 ，李香香 ，辛国鹏 ，杨晶莹 ，杨 薇 ，刘信平
（1. 湖北民族大学 化学与环境工程学院，湖北 恩施 445000；2. 湖北民族大学 生物科学与技术学院，
湖北 恩施 445000）
摘要：以二氢杨梅素（DMY）为底物、Na 2 SeO 3 为硒化剂制备了硒化 DMY。采用 UV-Vis、FTIR、NMR、XRD、
TG、原子荧光光谱表征其结构和性能。用 CCK-8 法检测了硒化 DMY 对人口腔鳞癌细胞 HSC-3 细胞增殖的影
响，通过划痕实验研究了硒化 DMY 对 HSC-3 细胞迁移的影响。结果表明，硒化 DMY 中仍存在黄酮基本母核，
并新形成 C—Se 键，其中硒含量为 6.54%±0.22%；DMY 和硒化 DMY 均对 HSC-3 细胞的增殖和迁移有良好的
抑制作用，抑制效果与其质量浓度呈正相关性，且 DMY 和硒化 DMY 对 HSC-3 细胞的半数抑制质量浓度（IC 50 ）
分别为 25.27、21.27 µg/mL，DMY 的硒化有效提高了其对 HSC-3 细胞增殖和迁移的抑制能力。
关键词：DMY；Na 2 SeO 3 ；硒化；抗肿瘤活性；医药原料
中图分类号：TQ460.1 文献标识码：A 文章编号：1003-5214 (2022) 05-0950-06

Preparation of selenizing DMY and its inhibition on
proliferation/metastasis of tumor cells

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MAO Min , LI Xiangxiang , XIN Guopeng , YANG Jingying , YANG Wei , LIU Xinping
（1. College of Chemistry and Environment Engineering, Hubei Minzu University, Enshi 445000, Hubei, China; 2. College
of Biological Science and Technology, Hubei Minzu University, Enshi 445000, Hubei, China）
Abstract: Selenizing dihydromyricetin (DMY) was prepared with DMY as substrate and Na 2SeO 3 as
selenizing agent. Its structure and performance were characterized by UV-Vis, FTIR, NMR, XRD, TG and
atomic fluorescence spectrometry. The effects of selenizing DMY on human oral squamous cell carcinoma
(HSC-3 cell) proliferation and human oral squamous cell carcinoma (HSC-3 cell) migration were tested by
CCK-8 method and scratch experiment, respectively. The results showed that there was still a basic nucleus
of flavonoids in selenizing DMY and new C—Se bonds were formed, and the selenium content was 6.54%±
0.22%. Both DMY and selenizing DMY had a good inhibitory effect on the proliferation and migration of
HSC-3 cells, and the inhibitory effect was positively correlated with the concentration. The half inhibitory
mass concentration (IC 50) of DMY and selenizing DMY on HSC-3 cells were 25.27 and 21.27 µg/mL,
respectively. The selenization of DMY effectively improved the ability to inhibit the proliferation and
migration of HSC-3 cells.
Key words: DMY; Na 2SeO 3; selenization; antitumor activity; drug materials

近年来恶性肿瘤发生率显著增加，且 90%恶性 良好，但患者常伴有脱发、恶心呕吐、血液中毒等严
[1]
肿瘤患者死亡是由于癌细胞转移 。临床化疗所用 重副作用 [2-3] 。研究发现，天然产物中的黄酮、生物
[4]
多种药物（如替莫唑胺、环磷酰胺、巯嘌呤）疗效 碱、二萜及皂苷等物质可抑制癌细胞增殖和迁移 ，

收稿日期：2021-12-02; 定用日期：2022-01-17; DOI: 10.13550/j.jxhg.20211239
基金项目：国家自然科学基金（21461009，21565013）
作者简介：毛 敏（1995—），女，硕士生，E-mail：min05eng@163.com。联系人：刘信平（1968—），女，教授，E-mail：363747611@
qq.com。