Page 133 - 《精细化工》2023年第11期

第 40 卷第 11 期 精 细 化 工 Vol.40, No.11
2 023 年 11 月 FINE CHEMICALS Nov. 2023

生物工程
芽孢杆菌 ZYCHH-01 发酵液提取物的

抗菌抗氧化性能评价及应用

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高亚惠，吴夏泠，杨丹敏，刘 群 ，翁武银，张玉苍
（集美大学 海洋食品与生物工程学院，福建 厦门 363100）

摘要：芽孢杆菌及其细胞外代谢物具有抗氧化、抗菌等性能，已被用于护肤品中。通过盐酸沉淀法从芽孢杆菌
ZYCHH-01 发酵产物中提取出活性物质，以自由基清除率为指标评价提取物的体外抗氧化活性，通过牛津杯扩
散实验评估提取物的抗菌能力，通过急性皮肤刺激和人体涂抹实验检测了添加提取物面霜的生物安全性。结果
表明，该提取物对 2,2-联苯基-1-苦基肼基自由基和 2,2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基具有一定
的清除活性，其半抑制浓度（IC 50 ）分别为 0.732 和 0.267 g/L；对革兰氏阳性菌和阴性菌均有抑制作用，可使细
菌形貌严重受损。在中国化妆品行业，单一的尼泊金酯最高质量分数为 0.4%，混合酯的最高质量分数为 0.8%，
当提取物质量浓度为 8 g/L（质量分数约为 0.8%）时，能有效抑制 6 种常见的致病菌生长，具有替代市售防腐
剂的可行性；且所制备的面霜具有安全性、无皮肤刺激性。
关键词：发酵提取物；芽孢杆菌 ZYCHH-01；抗氧化性；抗菌性；面霜；生物工程
中图分类号：TQ658.2 文献标识码：A 文章编号：1003-5214 (2023) 11-2445-09

Evaluation of antibacterial and antioxidant properties of Bacillus
ZYCHH-01 fermentation extract and its application on facial cream

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GAO Yahui, WU Xialing, YANG Danmin, LIU Qun , WENG Wuyin, ZHANG Yucang
（College of Ocean Food and Biological Engineering, Jimei University, Xiamen 363100, Fujian, China）

Abstract: Bacillus and its extracellular metabolites have been used in skin care products due to their
antioxidant and antibacterial properties. Herein, active substances from the fermentation product of Bacillus
ZYCHH-01 were extracted by hydrochloric acid precipitation method, with their in vitro antioxidant
activity evaluated using free radical scavenging rate as index, antibacterial performance assessed by Oxford
cup diffusion test, and the biosafety of the extract-added cream examined by acute skin irritation and human
application tests. The results showed that the extract exhibited certain scavenging activity against
2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radicals with
half maximal inhibitory concentration (IC 50) of 0.732 and 0.267 g/L, respectively. Meanwhile, the extract
inhibited the growth of both gram-positive and negative bacteria and severely destroyed the bacterial
morphology. In the Chinese cosmetic industry, the maximum amount (mass fraction) of single nipagin ester
and mixed ester used was 0.4% and 0.8%, respectively. The extract obtained with a mass concentration of
8 g/L (mass fraction about 0.8%) could effectively inhibit the growth of six common pathogenic bacteria,
indicating their strong potential to replace commercially available preservatives. The facial cream added
with the extract displayed biosafety and no skin irritation.
Key words: fermented extract; Bacillus ZYCHH-01; anti-oxidation; antimicrobial properties; facial cream;
biological engineering

收稿日期：2023-02-09; 定用日期：2023-05-09; DOI: 10.13550/j.jxhg.20230081
基金项目：国家自然科学基金项目（21978059）；福建省科技计划高校产学研合作项目（2021N5013）；科技部重点研发计划项目
（2021YFD210020202/2021YFD210020404）
作者简介：高亚惠（1996—），女，硕士生，E-mail：202011710035@jmu.edu.cn。联系人：刘 群（1990—），女，讲师，E-mail：
qunliu@jmu.edu.cn；张玉苍（1964—），男，教授，E-mail：yczhang@jmu.edu.cn。