Page 163 - 《精细化工》2023年第12期

第 40 卷第 12 期 精 细 化 工 Vol.40, No.12
2 023 年 12 月 FINE CHEMICALS Dec. 2023

催化与分离提纯技术
不同超声辅助提取桂花黄酮及其抑菌性

1
1
1*
2
2
邵越楣 ，李婷婷 ，吴彩娥 ，段一凡 ，王贤荣 ，
3
2
1
陈 林 ，李 旭 ，王 磊
（1. 南京林业大学 轻工与食品学院，江苏 南京 210000；2. 南京林业大学 生态与环境学院，江苏
南京 210000；3. 南京国环有机产品认证中心有限公司，江苏 南京 210000）
摘要：为了探究桂花黄酮的最佳提取工艺及其对白色念珠菌的抑菌效果，对超声提取法、超声辅助酶提取法和
超声辅助双水相提取法提取桂花黄酮的提取率进行了比较，获得提取率最高的方法，并通过单因素和响应面实
验优化提取工艺。采用超高效液相色谱-串联质谱（UPLC-MS/MS）对 4 个品种的桂花黄酮提取物进行了单体成
分的鉴定；并测定了 4 个品种桂花黄酮对白色念珠菌的最小抑菌浓度（MIC）及其对菌体细胞膜完整性和生物
膜形成的影响。结果表明，经超声提取桂花黄酮的提取率最高，在温度 50 ℃、液固比 17∶1（mL∶g）、乙醇
体积分数 52%的条件下，金桂、银桂、丹桂、四季桂的提取率分别为 13.88%±0.29%、8.79%±0.56%、13.16%±0.33%
和 10.28%±0.41%；4 种桂花黄酮中，金桂提取的黄酮对白色念珠菌的抑菌效果最好，MIC 为 1.50 g/L，能显著
提高白色念珠菌细胞膜的通透性，导致细胞内容物泄漏，抑制生物膜的形成，丹桂黄酮抑制率最高，可达 46.59%。
关键词：桂花；黄酮；响应面优化；抑菌活性；白色念珠菌；分离技术
中图分类号：R284.2；O657.63；TQ028 文献标识码：A
文章编号：1003-5214 (2023) 12-2705-09 开放科学 (资源服务) 标识码 (OSID)：

Osmanthus fragrans flavonoids extracted by different ultrasound-

assisted processes and its antibacterial activitiy

2
1
2
1*
1
SHAO Yuemei , LI Tingting , WU Cai'e , DUAN Yifan , WANG Xianrong ,
1
3
2
CHEN Lin , LI Xu , WANG Lei
（1. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210000, Jiangsu, China;
2. College of Ecology and Environment, Nanjing Forestry University, Nanjing 210000, Jiangsu, China; 3. Organic Food
Development and Certification Center of China Co., Ltd., Nanjing 210000, Jiangsu, China）
Abstract: In order to determine the optimal extraction process of Osmanthus fragrans flavonoids and their
antibacterial effect on Candida albicans (C. albicans), three methods, ultrasonic extraction, ultrasonic-
assisted enzyme extraction and ultrasonic-assisted double aqueous phase extraction, were compared for
their extraction rates of Osmanthus fragrans flavonoids. The method with the highest extraction rate was
selected for process optimization by single factor and response surface experiments. The components of
four kinds of Osmanthus fragrans flavonoids were identified by liquid chromatography-mass spectrometry
(UPLC-MS/MS). The inhibition effects of the four Osmanthus fragrans flavonoids on C. albicans in terms
of minimum inhibitory concentration (MIC), cell membrane integrity and biofilm formation were analyzed.
The results showed that, under the optimal extraction process of temperature 50 ℃, liquid-solid ratio 17∶1
(mL∶g), and ethanol volume fraction 52%, the extraction rates of Thunbergii group, Latifolius group,
Aurantiacus group, and Semperflorens group were 13.88%±0.29%, 8.79%±0.56%, 13.16%±0.33%, and
10.28%±0.41%, respectively. The four Osmanthus fragrans flavonoids displayed different antibacterial
effect on C. albicans, among which, Osmanthus fragrans var. thunbergii exhibited the best antifungal effect

收稿日期：2023-04-09; 定用日期：2023-06-06; DOI: 10.13550/j.jxhg.20230289
作者简介：邵越楣（1998—），女，硕士生，E-mail：shaoyuem@163.com。联系人：李婷婷（1982—），女，副教授，E-mail：
lttnjfu@njfu.edu.cn。