Page 109 - 《精细化工》2023年第5期

第 40 卷第 5 期 精 细 化 工 Vol.40, No.5
20 23 年 5 月 FINE CHEMICALS May 2023

功能材料
基于 β-CD@AuNPs-RB 的荧光探针

检测化妆品中的性激素

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董萌萌 ，王 婵 ，冉国侠 ，张 咪 ，宋启军
（1. 江南大学 化学与材料工程学院，江苏 无锡 214122；2. 江苏雪豹日化股份有限公司，江苏 无锡
214406）
摘要：以 β-环糊精（β-CD）和氯金酸为原料，制备了 β-环糊精包被的金纳米粒子（β-CD@AuNPs），通过 TEM、
FTIR、UV-Vis 和荧光光谱表征了 β-CD@AuNPs 的结构及光学特征。基于 β-CD@AuNPs 对罗丹明 B（RB）的
荧光猝灭、性激素（孕酮、雌二醇、睾酮）对 β-CD@AuNPs-RB 体系荧光的恢复现象，构建了高选择性、高灵
敏度的 β-CD@AuNPs-RB 荧光探针用于检测性激素。结果表明，0~7.5 μg/g 的孕酮与 β-CD@AuNPs-RB 的荧光
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恢复率呈良好的线性关系，线性相关系数（R ）为 0.992，检测限为 0.047 μg/g。另将此 β-CD@AuNPs-RB 用于
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检测雌二醇和睾酮，β-CD@AuNPs-RB 的荧光恢复率与 0~15 μg/g 的性激素呈良好的线性关系（R >0.99），检测
限分别为 0.15 和 0.37 μg/g。将 β-CD@AuNPs-RB 荧光探针用于爽肤水中性激素的测定，性激素回收率为
95.00%~104.67%，相对标准偏差≤4.7%。
关键词：性激素；孕酮；金纳米粒子；β-环糊精；罗丹明 B；功能材料
中图分类号：TQ617.3 文献标识码：A 文章编号：1003-5214 (2023) 05-1029-06

Fluorescent probe based on β-CD@AuNPs-RB for detection of

sex hormones in cosmetics

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DONG Mengmeng , WANG Chan , RAN Guoxia , ZHANG Mi , SONG Qijun
（1. School of Chemistry and Materials Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China; 2. Jiangsu
Snow Leopard Daily Chemical Co., Ltd., Wuxi 214406, Jiangsu, China）
Abstract: Gold nanoparticles coated with β-CD(β-CD@AuNPs) were synthesized from β-cyclodextrin
(β-CD) and chlorauric acid, and characterized by TEM, FTIR, UV-Vis and fluorescence spectra for
structural and optical property analysis. A fluorescence probe (β-CD@AuNPs-RB) with high selectivity and
sensitivity was then constructed for sex hormone detection based on the quenching of Rhodamine B (RB)
fluorescence by β-CD@AuNPs and the fluorescence recovery of β-CD@AuNPs-RB with sex hormone
(progesterone, estradiol, testosterone). The results showed that the correlation between fluorescence
recovery rate of β-CD@AuNPs-RB and progesterone in the range of 0~7.5 μg/g was linear with the linear
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correlation coefficient (R ) of 0.992 and the detection limit to 0.047 μg/g. Meanwhile, it was also found that
there was a good linear correlation between fluorescence recovery rate of β-CD@AuNPs-RB and estradiol
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or testosterone from 0 to 15 μg/g with R >0.99 and the detection limit of 0.15 and 0.37 μg/g,
respectively. Furthermore, when the β-CD@AuNPs-RB fluorescence probe was used for the determination
of sex hormone content in skin care toner, the recovery rate of sex hormone was 95.00%~104.67% and
relative standard deviation was≤4.7%.
Key words: sex hormones; progesterone; gold nanoparticles; β-cyclodextrin; Rhodamine B; functional
materials

收稿日期：2022-08-20; 定用日期：2022-11-23; DOI: 10.13550/j.jxhg.20220773
基金项目：国家自然科学基金（51973083）
作者简介：董萌萌（1997—），女，硕士生，E-mail：1320988516@qq.com。联系人：宋启军（1965—），男，教授，E-mail：qsong@
jiangnan.edu.cn。