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第 35 卷第 7 期 精 细 化 工 Vol.35, No.7
201 8 年 7 月 FINE CHEMICALS July 2018

中药现代化技术
木犀草素-Zn(Ⅱ)配位分子印迹聚合物的制备及应用

乐 薇，吉瑞冬，吴士筠

（武汉工商学院 环境与生物工程学院，湖北 武汉 430065）

摘要：以箬叶黄酮碳苷母核木犀草素为虚拟模板分子，结合 Zn(Ⅱ)的配位作用，丙烯酰胺为功能单体，N，N-
亚甲基双丙烯酰胺（MBA）为交联剂，乙醇为溶剂制备了木犀草素-Zn(Ⅱ)配位印迹聚合物，并将此聚合物与固
相萃取相结合，分离了醇水体系中的箬叶黄酮碳苷。结果表明：当 n（模板分子）∶n（功能单体）∶n（交联
剂）=1∶5∶30 时，配位印迹聚合物具有最好的吸附性能，对醇相中木犀草素的吸附容量可达 103.40 mg/g，是
未配位印迹聚合物吸附容量（60.61 mg/g）的 1.71 倍，空白印迹聚合物（7.33 mg/g）的 14.11 倍。采用木犀草素
-Zn(Ⅱ)配位印迹聚合物分离醇水相中箬叶黄酮碳苷，经一次分离后，荭草苷、异荭草苷、牡荆苷和异牡荆苷的
回收率分别为 91.95%、80.87%、60.43%和 63.63%；质量分数分别是粗提物的 55.36、48.89、36.64 和 38.35 倍，
4 种黄酮碳苷总质量百分数由提取前的 0.70%提高到 32.68%。
关键词：木犀草素；配位分子印迹聚合物；箬叶；黄酮碳苷；中药现代化技术
中图分类号：TQ041.8 R284.2 文献标识码：A 文章编号：1003-5214 (2018) 07-1156-07

Preparation and Application of Luteolin-Zn(Ⅱ)

Complex Molecularly Imprinted Polymers

YUE Wei, JI Rui-dong, WU Shi-jun
（College of Environmental and Biological Engineering, Wuhan Technology and Business University, Wuhan 430065,
Hubei, China）

Abstract: A series of luteolin-Zn(Ⅱ) complex molecularly imprinted polymers (MIPs) were synthesized in
ethanol, using luteolin (the mother nucleus of C-glycosyflavones from Indicalamus leaf) as dummy
template, acrylamide (AM) as monomer and N, N- methylene diacrylamide (MBA) as cross-linker. And
then C-glycosyflavones from Indicalamus leaf in alcohol-water system were separated by solid phase
extraction using MIPs. The results showed that when n(luteolin-Zn)∶n(AM)∶n(MBA) was 1∶5∶30, the
obtained complex molecularly imprinted polymer possessed the highest adsorption properties, and the
–1
adsorption capacity for luteolin from ethanol phase reached 103.40 mg·g , which was 1.71 times that of
uncoordinated molecularly imprinted polymer (60.61 mg/g), 14.11 times that of blank polymer (7.33 mg/g).
After a separation, the recovery rates of orientin, isoorientin, vitexin and isovitexin were 91.95%, 80.87%,
60.43% and 63.63%, compared with crude extract, the corresponding mass percents were increased by
55.36, 48.89, 36.64 and 38.35 times, respectively. In addition, the total mass percent of four C-glycosyflavones
was increased to 32.68% from 0.70% before extraction.
Key words: luteolin; complex molecularly imprinted polymer; Indicalamus leaf; C-glycosyflavones;
modernization technology of traditional Chinese medicines
Foundation items: Science Research Project of Hubei Provincial Department of Education (B2017321);
Academic Team Funding Project of Wuhan Technology and Business University (SXTD2015003)

箬叶是禾本科、箬竹属植物叶子的总称，主要 作食品包装物。箬叶来源丰富，具有抗菌、防腐、
分布于中国长江以南流域,又称为粽叶，目前主要用 抗癌等多种功效 [1~3] ，在开发功能性食品及药物方面

收稿日期：2017-08-02; 定用日期：2017-11-13; DOI: 10.13550/j.jxhg.20170629
基金项目：湖北省教育厅科学研究计划项目（B2017321），武汉工商学院校学术团队资助项目（SXTD2015003）
作者简介：乐 薇（1979—），女，副教授，E-mail：yuewei11@126.com